Intrusion on Spinal Gills of Maccullochella peelii by Ichthyophthirius multifiliis Observed Under Scanning Electron Microscope
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摘要: 应用扫描电镜对患小瓜虫病的澳洲龙纹斑的鳃丝及鳃小片的表面形态变化进行研究。结果表明，小瓜虫分布在澳洲龙纹斑鳃的扁平上皮细胞之下、鳃丝表面及鳃小片之间，感染小瓜虫后澳洲龙纹斑的鳃丝及鳃小片出现一定程度的膨胀变形，鳃丝小骨裸露变形，鳃小片变粗且间距缩小，鳃小片末端膨大变形，顶端充血呈球状或棒状，黏液细胞分泌增多，而且在鳃丝及鳃小片上观察到氯细胞的分泌增多。Abstract: Morphological changes on the filaments and lamella on gills of Maccullochella peelii infected by Ichthyophthirius multifiliis were observed under a scanning electron microscope. The invaded I.multifiliis could be found beneath the flat epithelial cells, on surface of the filaments, as well as in between the lamella of the gills. After being infected with the disease, the filaments and lamella on the gills began to swell and deform, the ossicles on the filaments became exposed and deformed, the lamella thickened, gaps narrowed, apical hyperemia enlarged with blood-filled spherical or rod-shaped tips, and the cellular mucous and chloride cell secretion increased.
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图 1 健康澳洲龙纹斑的鳃的扫描电镜观察
注：A为鳃丝整体观，×120；B为鳃丝及鳃小片，×180；C为鳃小片排列整齐，×450；D为鳃小片边缘，×6000。
Figure 1. Scanning electron microscope observation of gills of healthy Maccullochella peelii
图 2 小瓜虫感染后澳洲龙纹斑鳃的扫描电镜观察
注：A为鳃丝整体观, ×70；B为鳃丝及鳃小片肿胀，鳃小片缺失，×100；C为黏液细胞，×1700；D为鳃丝裸露，鳃小片末端肿胀，×350；E为鳃丝上的增生，×6000；F为鳃丝及鳃小片上的黏液及小瓜虫，×100；G为小瓜虫，×350；H为小瓜虫表面布满黏液及其他物质，×3500；I为鳃丝断裂，×250。
Figure 2. Scanning electron microscope observation of gills of Maccullochella peelii after infection of Ichthyophthirius mtltiflis
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