Effects of Piriformospora indica Treatment on Seed Germination and Seedling Growth of Longan Plants
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摘要: 以‘东壁’龙眼为材料，研究印度梨形孢对龙眼种子萌发和幼苗生长的影响。结果显示印度梨形孢处理极显著提高室温贮藏3 d的种子萌发率（P < 0.01）。播种后2个月统计结果显示印度梨形孢处理极显著提高龙眼幼苗侧根数量和地上部高度（P < 0.01），显著提高幼苗侧根、主根、地上部及幼苗总重（P < 0.05），对主根伸长有一定的抑制作用，但效果并不显著，对叶片数影响不大。说明对短期室温贮藏的龙眼种子而言，印度梨形孢处理能有效提高其萌发率，同时印度梨形孢定殖对龙眼幼苗生长有显著的促进作用。Abstract: Effects of Piriformospora indica treatment on seed germination and seedling growth of Dongbi longan (Dimocarpus longan) were investigated. After a treatment with P. indica, the germination rate of the longan seeds stored at room temperature for three days increased significantly at P < 0.01. Two months after sowing, the root count and height of the seedlings significantly increased as well (P < 0.01), and the weights of branched roots, main roots, above-ground and entire plantlet all significantly increased (P < 0.05). The average length of main roots on the treated seedlings was shorter than that on control, but not significantly different. Nor was there any significant difference on leaf count. It appeared that by treating the seeds with P. indica the germination rate and seedling growth of Dongbi longan could be improved.
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Key words:
- Dimocarpus longan /
- Piriformospora indica /
- seed germination /
- seedling growth
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图 1 印度梨形孢对‘东壁’龙眼种子萌发和幼苗生长的影响促进效果
注：A为对照组；B为印度梨形孢处理组。
Figure 1. Enhancement on seed germination and seedling growth of Dongbi longan by P. indica treatment
表 1 印度梨形孢对龙眼种子萌发和幼苗生长的影响
Table 1. Effects of P. indica treatment on seed germination and seedling growth of Dongbi longan
测定指标 对照组 处理组 种子萌发率/% 54.2±13.0 95.8±3.6** 叶片数 9.50±0.91 10.20±0.63 地上部高度/cm 7.42±1.766 10.10±1.437** 主根长/cm 15.83±2.25 12.83±1.61 侧根数目 10.00±3.46 34.33±4.93** 地上部重量/g 0.86±0.07 1.06±0.10* 侧根重量/g 0.09±0.02 0.14±0.01* 主根重量/g 0.37±0.02 0.41±0.01* 总重量/g 1.32±0.05 0.60±0.10* 注：与对照组比较*P < 0.05(差异显著)，**P < 0.01(差异极显著)。 
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[1] 张春叶, 朱凤林.我国龙眼品种资源, 栽培技术及生理研究的进展[J].福建农业大学学报, 1999, 28(2):157-162. http://www.cnki.com.cn/Article/CJFDTOTAL-YYXB198403004.htm [2] LIN Y, MIN J, LAI R, et al. Genome-wide sequencing of longan (Dimocarpus longan Lour.) provides insights into molecular basis of its polyphenol-rich characteristics[J]. Giga Science, 2017, 6(5):1-14. [3] VERMA S, VARMA A, REXER K H, et al. Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus[J]. Mycologia, 1998:896-903. http://cn.bing.com/academic/profile?id=680788717d8fde585d160dc623143f47&encoded=0&v=paper_preview&mkt=zh-cn [4] 林觅. 印度梨形孢对非洲菊生长、抗病性及Snakin基因表达的影响[D]. 福州: 福建农林大学, 2017. [5] 齐全. 印度梨形孢对香蕉生长、抗病性及Snakin基因表达的影响[D]. 福州: 福建农林大学, 2017. [6] VARMA A, BAKSHI M, LOU B, et al. Piriformospora indica:a novel plant growth-promoting mycorrhizal fungus[J]. Agricultural Research, 2012, 1(2):117-131. doi: 10.1007/s40003-012-0019-5 [7] 吴金丹, 陈乾, 刘晓曦, 等.印度梨形孢对水稻的促生作用及其机理的初探[J].中国水稻科学, 2015, 29(2):200-207. http://www.ricesci.cn/article/2015/1001-7216-29-2-200.html [8] 马杰. 印度梨形孢诱导烟草促生, 抗病, 抗逆作用及其机理的初步研究[D]. 杭州: 浙江大学, 2012. http://cdmd.cnki.com.cn/Article/CDMD-10335-1013187009.htm [9] 王慧俐. 印度梨形孢Piriformospora indica对果蔬生长, 品质及抗病性的影响及其相关机制研究[D]. 杭州: 浙江大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10335-1015399873.htm [10] 孙超. 印度梨形孢诱导小白菜抗病、促生、抗逆的作用及其机理的初步研究[D]. 杭州: 浙江大学, 2010. http://cdmd.cnki.com.cn/Article/CDMD-10335-2010138913.htm [11] 王凤让, 毛克克, 李国钧, 等.印度梨形孢及其近似种Sebacina vermifera促进番茄生长发育及磷吸收[J].浙江大学学报(农业与生命科学版), 2011, 37(1):61-68. http://kns.cnki.net/KCMS/detail/detail.aspx?filename=zjny201101010&dbname=CJFD&dbcode=CJFQ [12] VARMA A, VERMA S, SAHAY N, et al. Piriformospora indica, a cultivable plant-growth-promoting root endophyte[J]. Applied and Environmental Microbiology, 1999, 65(6):2741-2744. http://cn.bing.com/academic/profile?id=e28a8fff0e768695b216e450d86eb00c&encoded=0&v=paper_preview&mkt=zh-cn [13] PEŠKAN-BERGHÖFER T, SHAHOLLARI B, GIONG P H, et al. Association of Piriformospora indica with Arabidopsis thaliana roots represents a novel system to study beneficial plant microbe interactions and involves early plant protein modifications in the endoplasmic reticulum and at the plasma membrane[J]. Physiologia Plantarum, 2004, 122(4):465-477. doi: 10.1111/ppl.2004.122.issue-4 [14] SHENDE S, BHAGWAT K, WADEGAONKAR P, et al. Piriformospora indica as a new and emerging mycofertilizer and biotizer: potentials and prospects in sustainable agriculture[M]. Handbook of microbial biofertilizers, Food products press, New York, 2006: 477-496. [15] WALLER F, ACHATZ B, BALTRUSCHAT H, et al. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(38):13386. doi: 10.1073/pnas.0504423102 [16] FAKHRO A, ANDRADE-LINARES D R, VON BARGEN S, et al. Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens[J]. Mycorrhiza, 2010, 20(3):191-200. doi: 10.1007/s00572-009-0279-5 [17] SCHÄFER P, PFIFFI S, VOLL L M, et al. Manipulation of plant innate immunity and gibberellin as factor of compatibility in the mutualistic association of barley roots with Piriformospora indica[J]. The Plant Journal, 2009, 59(3):461-474. doi: 10.1111/tpj.2009.59.issue-3 [18] YE W, SHEN C H, LIN Y, et al. Growth promotion-related miRNAs in Oncidium orchid roots colonized by the endophytic fungus Piriformospora indica[J]. PLoS One, 2014, 9(1):e84920. doi: 10.1371/journal.pone.0084920 [19] 牛雨晴, 许惠滨, 张雨潇, 等.植物种子休眠分子机制研究进展[J].福建农业学报, 2017, 32(10):1156-1164. http://www.cnki.com.cn/Article/CJFDTOTAL-YOKE200403000.htm [20] 楼兵干, 孙超, 蔡大广.印度梨形孢的多种功能及其应用前景[J].植物保护学报, 2007(6):653-656. http://www.cqvip.com/qk/90215X/20076/26416400.html [21] LIU Z L, LI Y J, HOU H Y, et al. Differences in the arbuscular mycorrhizal fungi-improved rice resistance to low temperature at two N levels:aspects of N and C metabolism on the plant side[J]. Plant Physiology and Biochemistry, 2013, 71:87-95. doi: 10.1016/j.plaphy.2013.07.002 [22] RAI M, VARMA A. Arbuscular mycorrhiza-like biotechnological potential of Piriformospora indica, which promotes the growth of Adhatoda vasica Nees[J]. Electronic Journal of Biotechnology. 2005, 8(1):107-112. http://cn.bing.com/academic/profile?id=3067bf30737bfb50b0025415b71c4056&encoded=0&v=paper_preview&mkt=zh-cn [23] ANSARI M W, TRIVEDI D K, SAHOO R K, et al. A critical review on fungi mediated plant responses with special emphasis to Piriformospora indica on improved production and protection of crops[J]. Plant physiology and biochemistry, 2013, 70:403-410. doi: 10.1016/j.plaphy.2013.06.005 [24] FRANKEN P. The plant strengthening root endophyte Piriformospora indica:potential application and the biology behind[J]. Applied microbiology and biotechnology, 2012, 96(6):1455-1464. doi: 10.1007/s00253-012-4506-1 [25] WALLER F, ACHATZ B, BALTRUSCHAT H, et al. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(38):13386. doi: 10.1073/pnas.0504423102 -
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