Aromatic Volatiles in Lentinula edodes Determined by Gas Chromatography-Ion Mobility Spectrometry
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摘要: 利用气相色谱-离子迁移谱（gas chromatography-ion mobility spectrometry，GC-IMS）技术分析不同香菇干样挥发性组分的指纹图谱。根据离子迁移数据利用热图聚类和主成分分析不同样品间的差异。热图聚类分析可将27份香菇材料分为2大类；PCA分析表明，前2个主坐标的方差贡献率分别为30.2%和26.9%。结果表明气相色谱偶联离子迁移谱技术对构建香菇挥发性物质指纹图谱及挥发性组分数据库具有潜在的应用价值。Abstract: Gas chromatography-ion mobility spectrometry (GC-IMS) was applied to analyze the aromatic components in 27 Lentinula edodes. The measurement data were subsequently subjected to the principal component, clustering and heatmap analyses. Accordingly, the mushrooms were clustered into two groups. Two principal components of the volatiles contributed 30.2% and 26.9% of the total variance among the mushroom samples. The analytical method and the established fingerprints on the chemical components would be useful for future studies on L. edodes.
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Key words:
- Lentinula edodes /
- volatile components /
- ion mobility spectrometry /
- gas chromatography
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图 2 香菇样品挥发性组分热图聚类分析
Figure 2. Heatmap and accompanying cluster analyses on volatiles in L. edodes
表 1 挥发性组分的定性
Table 1. Volatiles identified by GC-IMS
物质名称 CAS号 保留时间
/s保留指数 漂移时间
/ms环己酮 C108941 223.162 885.3 1.1543 顺式-3-己烯-1-醇 C928961 210.023 866.8 1.6604 2-甲基丁酸-甲基丁酯 C868575 142.379 753.5 1.5251 乙酸乙酯 C141786 84.148 583.6 1.3291 甲基丙醛 C78842 81.549 571.4 1.2826 
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[1] LIU Y, LEI X Y, CHEN L F, et al. A novel cysteine desulfurase influencing organosulfur compounds in Lentinula edodes [J]. Sci Rep, 2015(5): 10047. http://cn.bing.com/academic/profile?id=d47d948d59dc1c9049149ce36417c577&encoded=0&v=paper_preview&mkt=zh-cn [2] 温泉, 王锡昌.食用菌风味物质的研究及应用进展[J].长江大学学报(自然科学版), 2006, 3(11): 211-213. http://mall.cnki.net/magazine/Article/ZSYJ201303003.htm [3] ZHAEG Y, HAO M, NAN H, et al. Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies[J]. Pakistan J Pharm Sci, 2015, 28(3): 1069-1074. https://www.researchgate.net/profile/Jeff_Bekomo/publication/277894330_Relationship_of_chemical_composition_and_cytotoxicity_of_water-soluble_polysaccharides_from_Lentinus_edodes_fruiting_bodies/links/55c2bf8208aea2d9bdbfeed5.pdf [4] BISEN P S, BAGHEL R K, SANODIYA B S, et al. Lentinus edodes: A macrofungus with pharmacological activities[J]. Curr Med Chem, 2010, 17(22): 2419-2430. doi: 10.2174/092986710791698495 [5] NAGASHIMA Y, YOSHINO S, YAMAMOTO S, et al. Lentinula edodes mycelia extract plus adjuvant chemotherapy for breast cancer patients: Results of a randomized study on host quality of life and immune function improvement[J]. Mol Clin Oncol, 2017, 7(3): 359-366. doi: 10.3892/mco.2017.1346 [6] 谷镇. 食用菌呈香呈味物质分析及制备工艺研究[D]. 上海: 上海师范大学, 2012. [7] 王文亮, 孙卿, 曹世宁, 等.香菇风味物质形成机理研究进展[J].山东农业科学, 2015, (6): 145-147. http://www.cnki.com.cn/Article/CJFDTotal-AGRI201506035.htm [8] 杨俊超, 曹树亚, 杨柳.气相色谱与离子迁移谱仪联用的研究[J].现代仪器与医疗, 2014, (3): 20-24. http://www.cnki.com.cn/Article/CJFDTOTAL-XDYI201403006.htm [9] GARRIDODELGADO R, DOBAOPRIETO M M, ARCE L, et al. Determination of volatile compounds by GC-IMS to assign the quality of virgin olive oil[J]. Food Chem, 2015, 187:572. doi: 10.1016/j.foodchem.2015.04.082 [10] KRISILOVA E V, LEVINA A M, MAKARENKO V A. Determination of the volatile compounds of vegetable oils using an ion-mobility spectrometer[J]. J Anal Chem, 2014, 69(4):371-376. doi: 10.1134/S1061934814020075 [11] LI Q, LI R, CAO G, et al. Direct differentiation of herbal medicine for volatile components by a multicapillary column with ion mobility spectrometry method[J]. J Sep Sci, 2015, 38(18):3205-3208. doi: 10.1002/jssc.201500402 [12] TAUNO M, JAAK V. Clustvis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap[J]. Nucleic Acids Res, 2015, 43(W1): W566-W570. doi: 10.1093/nar/gkv468 [13] 葛含光, 温华蔚, 宋旭, 等.离子迁移谱法检测蒸馏酒中4种风味成分[J].食品安全质量检测学报, 2016, 7(2): 834-838. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spaqzljcjs201602066 -
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