小菜蛾饲料品系转食寄主植物的适合度及其硫苷代谢相关基因表达

董玉红; 陈玮; 郑玲; 荆晓东; 周立; 张玲玲; 李晓桐; 何玮毅

doi:10.19303/j.issn.1008-0384.2018.01.011

小菜蛾饲料品系转食寄主植物的适合度及其硫苷代谢相关基因表达

doi: 10.19303/j.issn.1008-0384.2018.01.011

董玉红^{1, 2, 3,},
陈玮^{1, 2, 3},
郑玲^{1, 2, 3},
荆晓东^{1, 2, 3},
周立^{1, 2, 3},
张玲玲^{1, 2, 3},
李晓桐^{1, 2, 3},
何玮毅^{1, 2, 3, ,}

1.
闽台作物有害生物生态防控国家重点实验室/福建农林大学应用生态研究所, 福建福州 350002
2.
农业部闽台作物有害生物综合治理重点实验室/福建农林大学, 福建福州 350002
3.
闽台作物有害生物生态防控协同创新中心/福建农林大学, 福建福州 350002

基金项目:

国家重点研发计划 2017YFD0200400

福建省自然科学基金项目 2014J01086

福建农林大学“校杰出青年科研人才”项目 xjq201403

详细信息

作者简介:
董玉红(1990-), 女, 硕士, 主要从事昆虫与寄主互作研究(E-mail:550462218@qq.com)

通讯作者:
何玮毅(1982-), 男, 博士, 副教授, 主要从事昆虫与寄主互作研究(E-mail:wy.he@fafu.edu.cn)

中图分类号: Q966
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出版历程
- 收稿日期: 2017-11-01
- 修回日期: 2017-12-12
- 刊出日期: 2018-01-01

Fitness and Glucosinolate Catabolism-related Gene Expression Upon Feeding on Natural Host Plant of Plutella xylostella Raised from Artificial Diet

DONG Yu-hong^{1, 2, 3
,},
CHEN Wei^{1, 2, 3},
ZHENG Ling^{1, 2, 3},
JING Xiao-dong^{1, 2, 3},
ZHOU Li^{1, 2, 3},
ZHANG Ling-ling^{1, 2, 3},
LI Xiao-tong^{1, 2, 3},
HE Wei-yi^{1, 2, 3
, ,}

1.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology/Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, Fujian 350002, China
3.
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests/Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要

摘要: 硫代葡萄糖苷硫酸酯酶（GSS）及其修饰因子（SUMF1）是小菜蛾代谢十字花科植物化学防御物质硫苷的关键因子，但其在人工饲料品系小菜蛾适应寄主植物方面的功能研究中鲜有报道。本研究检测了硫苷代谢相关基因GSS和SUMF1在2种人工饲料品系小菜蛾（AD和G88）不同发育阶段的表达模式，发现GSS基因在2种饲料品系间的表达模式较为相似，GSS1和GSS2均在3龄和4龄幼虫中大量表达；SUMF1基因的表达模式则差别较大，未呈现明显规律性。AD和G88品系小菜蛾从初孵幼虫起被转移至萝卜子叶上饲养，与取食人工饲料相比幼虫存活率下降、发育历期延长、蛹重降低；幼虫中肠的GSS1和GSS2表达水平显著下降，AD品系的SUMF1a基因在幼虫中肠的表达亦显著下降。本研究通过探究饲料品系小菜蛾转食寄主植物后适合度与GSS和SUMF1基因表达水平的关系，揭示了硫苷代谢相关基因的表达水平很可能受到植物因子调控，并与人工饲料品系小菜蛾对寄主植物的适应性密切相关。
- 小菜蛾 /
- 硫代葡萄糖苷硫酸酯酶 /
- 硫酸酯酶修饰因子 /
- 人工饲料品系 /
- 转寄主
Abstract: Glucosinolate sulfatase (GSS) and sulfatase modifying factor 1 (SUMF1) are crucial for the catabolism of Plutella xylostella on the defensive glucosinolates in the cruciferous host plants. However, little information is available on their roles in the adaptation of the strain of P. xylostella raised from an artificial diet when changed to be fed on its natural host plant. The expression patterns of GSSs and SUMF1s at different developmental stages of two artificial diet strains of P. xylostella (i.e., AD and G88) were determined. It was found that the expressions of GSSs were similar between the two strains, with abundant GSS1 and GSS2 expressions at the 3^rd and 4^th-instar stages, but no apparent patterns observed for SUMF1s. After the newly hatched larvae of AD and G88 were transferred onto the cotyledons of radish plants, the larval survival rates became lower, with longer larval developmental time and lower pupal weight, than their counterparts fed on the original artificial diet. The expression levels of GSS1 and GSS2 in the larval midguts grown on the radish cotyledons decreased significantly; but, that of SUMF1a, only in the midguts of AD strain. It suggested that the expressions of glucosinolate catabolism-related genes in P. xylostella were possibly regulated by the factor(s) in the host plant and closely associated with the adaptability of the insects upon a shifted feeding from a formulated diet to a natural host plant.
- Plutella xylostella /
- glucosinolate sulfatase /
- sulfatase modifying factor 1 /
- artificial diet strain /
- host shift

HTML全文

图 1 GSS1和GSS2在不同发育阶段的表达模式

注：A、B、C、D分别为AD-GSS1、G88-GSS1、AD-GSS2、G88-GSS2的表达模式。EGG为卵，L1~L4为幼虫1~4龄期，P为蛹，FA为雌性成虫，MA为雄性成虫。数据以平均值+标准误的形式呈现于图表，不同小写字母表示数据经单因素方差分析后，利用Tukey多重比较检测在0.05水平下存在显著差异。图 2同。

Figure 1. Stage-specific expression patterns of GSS1 and GSS2

下载: 全尺寸图片幻灯片

图 2 SUMF1a和SUMF1b在不同发育阶段的表达模式

注：A、B、C、D分别为AD-SUMF1a、G88-SUMF1a、AD-SUMF1b、G88-SUMF1b的表达模式。

Figure 2. Stage-specific expression patterns of SUMF1a and SUMF1b

下载: 全尺寸图片幻灯片

图 3 转换寄主对小菜蛾存活率、发育历期和蛹重的影响

注：A、D分别为G88品系和AD品系幼虫存活率，B、E为幼虫发育历期，C、F为蛹重。G88(AD)-Gck为长期取食人工饲料的小菜蛾G88(AD)品系，G88(AD)-Trans-G1、G2为G88(AD)品系小菜蛾初孵幼虫取食萝卜子叶第一代、第二代。A和D采用Log-rank (Mantel-Cox)分析方法进行两两比较，B、E、C、F采用独立样本t检验；*表示P < 0.05，***表示P < 0.001。

Figure 3. Effect of host shift on survival rate, developmental time and pupal weight of artificial diet stain of P. xylostella

下载: 全尺寸图片幻灯片

图 4 饲料品系取食萝卜子叶后GSS1和GSS2基因表达模式

注：A、B为GSS1基因，C、D为GSS2基因。AD(G88)-Gck为长期取食人工饲料的AD(G88)小菜蛾品系4龄幼虫中肠，AD (G88)-Trans-G1、G2为AD(G88)品系小菜蛾初孵幼虫取食萝卜子叶第一代、第二代的4龄幼虫中肠，数据分析方法与图 1相同。*P < 0.05，** P < 0.01。图 5同。

Figure 4. Expression patterns of GSS1 and GSS2 in artificial diet stains after feeding on radish cotyledons

下载: 全尺寸图片幻灯片

图 5 饲料品系取食萝卜子叶后SUMF1a和SUMF1b基因表达模式

注：A、B为SUMF1a基因，C、D为SUMF1b基因。

Figure 5. Expression patterns of SUMF1a and SUMF1b in artificial diet stains after feeding on radish cotyledons

下载: 全尺寸图片幻灯片

表 1 荧光定量PCR引物序列

Table 1. Sequences of primer pairs used for q-PCR

基因	引物对		产物长度/bp
基因	序列	退火温度/℃	产物长度/bp
GSS1	F: AGGACCCTTGTGAGCTTCGT R: ACTTGGGGTCAGCGACGT	58	129
GSS2	F: CGGACCCTTGCGAGCTGCGA R: CCCTGGGGTCAGCGGTGA	58	129
SUMF1a	F: CATAGAAGCGGACAACGAGG R: TCCACGAACTCACTGAAATC	58	102
SUMF1b	F: ACATTTCCCAGCCATAACTC R: ACTCCCAGACATTCCCGACA	58	112
RPL32	F:CAATCAGGCCAATTTACCGC R:CTGGGTTTACGCCAGTTACG	58	109

下载: 导出CSV

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