Immunological Effect on Egg-laying Chickens by Combining Vaccinations Against Newcastle and Infectious Bursal Diseases
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摘要: 为探讨新城疫-传染性法氏囊病二联灭活疫苗（ND-IBD二联灭活苗）对不同日龄商品蛋鸡的免疫效果，选择360只1日龄商品蛋鸡，分别于1、7日龄接种ND-IBD二联灭活苗，对照组接种灭菌生理盐水，分别于21-56日龄，每隔7 d采血，分离血清，检测ND和IBD抗体水平，剖检取脾脏与法氏囊观察病变，荧光定量PCR检测免疫因子CD4+、CD8+、IL-6、IFN-βmRNA表达量，并进行攻毒保护试验。结果表明，1、7日龄商品蛋鸡接种ND-IBD二联灭活苗，其ND和IBD的抗体水平均有效提高，且在免疫后可以有效保护IBDV强毒的感染，但7日龄免疫效果更好。免疫因子的mRNA表达量检测结果表明，7日龄接种疫苗的免疫组免疫因子CD4+、CD8+、IL-6、IFN-β的mRNA表达量明显高于对照组1日龄。综上，ND-IBD二联灭活疫苗7日龄免疫商品蛋鸡免疫效果更好。Abstract: Immunological effect of combined inoculation of the inactivated vaccines for Newcastleand the infectious bursal diseases (ND-IBD) on egg-laying chickens was investigated. For the study, 360 one-day-old commercial chicks were randomly divided into 3 groups. The chicks in one group were immunized immediately with the two vaccines simultaneously, another when they were 7-day-old, and the third group injected with saline solution as control.Levels of ND-IBD antibodies in the serum ofthese chicks were determined, spleen biopsies for disease detection executed, and qPCR on mRNA expressions of CD4+, CD8+, IL-6 and IFN-β performed every 7 days during the period of 21 to 56 d. The results showed that all treated one-or 7-day-old birds had higher antibody levels than control, and could fence off IBD virus infection. The 7-day-old group showed greater immunity against the diseases, and significantly increased mRNA expressions on CD4+, CD8+, IL-6 and IFN-β over control. It was, therefore, concluded that, by combining vaccinations against ND-IBD on 7-day-old egg-laying chicks, a desirable immunological effect on the chickens could be achieved.
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Key words:
- newcastle disease /
- infectious bursal disease /
- inactivated vaccine /
- antibody detection
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图 1 不同处理IBD抗体水平
注：不同小写字母表示同一日龄不同处理间差异显著(P < 0.05)，下图同。
Figure 1. Levels of antibody against IBD in treatment groups
图 3 法氏囊组织中基因CD4、CD8、IL-6、IFN-β表达水平
注：A为商品蛋鸡法氏囊中CD4+基因表达量变化水平；B为商品蛋鸡法氏囊中CD8+基因表达量变化水平；C为商品蛋鸡法氏囊中IL-6基因表达量变化水平；D为商品蛋鸡法氏囊中IFN-β基因表达量变化水平。图 4同。
Figure 3. mRNA expressions of CD4+, CD8+, IL-6 and IFN-β in bursa tissue of chicks
图 4 各日龄鸡脾脏组织中基因CD4、CD8、IL-6、IFN-β表达量变化
Figure 4. mRNA expressions of CD4+, CD8+, IL-6 and IFN-β in spleen of chicks
表 1 引物序列
Table 1. Sequences of primers for RT qPCR
基因 引物序列(5′-3′) 扩增产物大小/bp CD4-F 5-GCAGTGGTGAGCTGTGTGTT-3 106 CD4-R 5-TCACCTCCTTTCCTGCAATC-3 CD8-F 5-CAGGGACAGAGGAACACGAT-3 125 CD8-R 5-CGAATCCAGGAGACACCATT-3 IL-6-F 5-ACCCAGAAATCCCTCCTCAC-3 126 IL-6-R 5-AAATAGCGAACAGCCCTCAC-3 IFN-β-F 5-GCTCTCCTGTTGTGCTTCTCCAC-3 120 IFN-β-R 5-CAATAGTCTCATTCCAGCCAGTGC-3 GAPDH-F 5-GCAGGAACATCCCGAAGAAGC-3 191 GAPDH-R 5-CCCGGAAGTGACCATGAGTAG-3 
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表 2 攻毒保护试验结果
Table 2. Survival rate of chicken artificially infected with IBDV
日龄/d 攻毒保护率/% A组(对照组) B组(1日龄免疫组) C组(7日龄免疫组) 21 25 70 80 28 5 80 90 35 0 100 100 42 0 100 100 49 0 100 100 56 0 100 100
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[1] MULLER H, ISLAM M R, RAUE R. Research on infectious bursal disease the past, the present and the future[J]. Vet Microbiol, 2003, 97(1/2):153-165. https://www.sciencedirect.com/science/article/pii/S037811350300275X [2] 王志全, 丁鹏.新城疫研究进展及综合防治[J].中国畜禽种业, 2014, 10(2):136-137. http://d.wanfangdata.com.cn/Periodical/zhonggnywz-xm201402102 [3] 李成洪, 王建华, 段霖.蜂胶提取物对鸡传染性法氏囊病疫苗免疫作用的影响[J].黑龙江畜牧兽医, 2017, (8):151-154. [4] 梅永杰, 王永山, 欧阳伟, 等.华东地区传染性法氏囊病病毒流行株的分离及其生物学特性分析[J].中国兽医科学, 2016, 46(5):550-557. http://www.cqvip.com/QK/93994X/201605/668851100.html [5] 李潭清, 宋勤叶, 杨润德, 等.鸡传染性法氏囊病的研究进展[J].安徽农业科学, 2008, 36(35):15481-15484. doi: 10.3969/j.issn.0517-6611.2008.35.082 [6] 江兴华, 林晓荣, 星东, 等.鸡新城疫、传染性法氏囊病二联灭活疫苗免疫效果研究[J].黑龙江畜牧兽医, 2017, (3):108-110. http://d.wanfangdata.com.cn/Periodical/dwbj201705039 [7] 于晓雪. 鸡CD4+CD25+细胞在传染性法氏囊病和免疫耐受诱导过程中的作用[D]. 北京: 中国农业大学, 2016. [8] 闫若潜, 谢彩华, 吴志明, 等. 重组鸡白细胞介素-6(rChIL-6)对不同种类疫苗免疫增强作用研究[C]//中国畜牧兽医学会禽病学分会第十五次学术研讨会论文集. 2010. [9] 张忠民. IL-6与NF-κB在人脑胶质瘤中的表达及相关性研究[D]. 佳木斯: 佳木斯大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10227-1015803780.htm [10] 陈建忠, 朱海红, 陈智, 等. IFN-α/β信号传导研究进展[J].国际流行病学传染病学杂志, 2003, 30(5):266-268. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_gwyx-lxbxcrbxfc200305004 [11] 孙恒.鸡新城疫的疫苗接种方式与免疫程序[J].养殖技术顾问, 2012, (3):206-206. http://d.wanfangdata.com.cn/Periodical/yzjsgw201203211 [12] 赵占民, 赵来兵, 李全福, 等.母源抗体对鸡新城疫疫苗免疫的影响[J].中国兽医杂志, 2004, 40(3):6-8. http://d.wanfangdata.com.cn/Periodical/zgsyzz200403002 [13] 章振华, 李林, 景小冬, 等.鸡传染性法氏囊病免疫复合物疫苗对1日龄中等母源抗体水平雏鸡免疫效果的研究[J].中国畜牧兽医, 2015, 42(12):3338-3343. http://d.wanfangdata.com.cn/Periodical/zgxmsy201512033 [14] 简子健, 曹竹亭, 马素贞, 等.单稀释法间接ELISA和终点法中和试验检测犊牛轮状病毒疫苗抗体效价比较[J].新疆农业科学, 2008, 45(4):733-737. http://d.wanfangdata.com.cn/Periodical/xjnykx200804032 [15] 侯继波, 何家惠, 惠艳华, 等.鸡传染性支气管炎病毒血凝抑制试验研究[J].中国家禽, 2007, 29(1):20-22. http://d.wanfangdata.com.cn/Periodical/zgjq200701006 [16] 黄劲, 邓永荣, 戴荣旺, 等.中药制剂对禽流感病毒H9N2、新城疫病毒、传染性支气管炎病毒和鸡毒支原体的攻毒保护试验[J].动物医学进展, 2005, 26(12):83-86. doi: 10.3969/j.issn.1007-5038.2005.12.021 [17] 孙德君, 丁国杰, 藏玉婷, 等.鸡传染性法氏囊病特点、诊断及国内外灭活疫苗免疫效力比较[J].科技与实践, 2012, 50(20):76-78. http://d.wanfangdata.com.cn/Periodical/zgxmzz201420015 [18] 王秀荣, 王笑梅, 吴东来, 等.鸡贫血病灭活疫苗免疫后雏鸡免疫状况研究[J].中国预防兽医学报, 2000, 23(S1):63-65. [19] 季卫国, 方占海, 周月琴.鸡传染性法氏囊病的综合防控[J].养殖与饲料, 2015, (8):52-53. http://d.wanfangdata.com.cn/Periodical/yzysl201508026 [20] RAUTENSCHLEINS, YEH H Y, SHARMA J M. The role of T cells in protection by an inactivated infectious bursal disease virus vaccine[J].Veterinary immunology and immunopathology, 2002, 89(3/4):159-167. [21] 刘蕊娜, 邹年莉, 王红宁, 等.鸡IL-6真核表达载体的构建及其对新城疫LaSota疫苗的免疫增强作用研究[J].畜牧兽医学报, 2009, 40(1):93-97. http://d.wanfangdata.com.cn/Periodical/xmsyxb200901016 [22] BLOCK H, MEYE R-BLOCK K, REBESKID, et al. A field study on the significance of vaccination against infectious bursal disease virus (IBDV) at the optimal time point in broiler flocks with maternally derived IBDV antibodies[J].Avianpathology, 2007, 36(5):401-409. [23] 梁华丽, 华炯钢, 徐辉, 等.几种鸡新城疫油佐剂灭活疫苗免疫效果的比较[J].浙江农业学报, 2007, 19(6):418-422. http://d.wanfangdata.com.cn/Periodical/zjnyxb200706005 -
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