Molecular Epidemiology of Enzootic Nasal Tumor Virus on Goats in Fujian
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摘要: 为明确2014-2017年福建省多地区羊发生的一种以流稀薄鼻液为主要症状疾病的病原，对该病进行流行病学调查、肿瘤组织切片观察，特异性PCR诊断以及核酸序列分析，结果显示该病在流行病学、临床症状、病理变化、组织病理变化上与国内外已报道的病例表现基本一致，RT-PCR鉴定结果为ENTV-2，其中7个分离株与ENTV-2CHN2（分离于中国）处于同一进化树分支，1个分离株与ENTV-SC（分离于中国）、ENTV-Shaanxi（分离于中国）处于同一进化树分支。说明近几年福建省流行的羊肿瘤病的病原为ENTV-2，为福建省加强该病的防治奠定基础。Abstract: The pathogen that caused a disease characterized by thin nasal discharge on goats in Fujian during 2014-2017 was investigated.An epidemiological survey of the disease was conducted with tumor tissue biopsy, specific PCR diagnosis, and nucleic acid sequence analysis. It was found that the disease had basically the sam eepidemiology, clinical symptoms, pathology, and histopathology as the similar cases reported domestically and abroad. RT-PCR identified the pathogen to be ENTV-2. Among the various isolated viruses, 7 were in the same phylogenetic tree branch as ENTV-2 CHN2, and one in the branch as ENTV-SC and ENTV-Shaanxi, which were all isolated in China.The results confirmed ENTV-2 to be the culprit of the disease in goats found in Fujian Province. Hopefully, with the information, a prevention measure and/or cure for the disease could be developed in the near future.
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Key words:
- goat /
- enzootic nasal tumor /
- molecular epidemiology
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图 2 羊头部病理解剖
注：鼻甲骨出血，筛骨萎缩。鼻腔中有腺瘤，呈菜花状、结节状或类圆柱状，周围无包膜，外观呈粉红色、表面不平、质地软脆，额窦内有积液或胶冻样渗出物。
Figure 2. Pathological anatomy of goat head
图 3 羊鼻内肿瘤部分病例PCR结果
注：M为DNA Marker 2000；NC为空白对照；1~5、6~16为部分病例。
Figure 3. PCR detection onenzootic nasal tumor virus of goats in clinic samples
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