Physiological Indices on Top-leaves and Pseudo-bulbs of Oncidium at Different Growth Stages
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摘要: 以3年生文心兰‘金辉’为试验材料，测定其4个不同生育期顶叶和假鳞茎中叶绿素、相对电导率、可溶性糖、还原糖、淀粉、脯氨酸（Pro）和过氧化物酶（POD）等生理指标。结果表明：膨大期顶叶叶绿素含量最低，而相对电导率则最高，两者随后趋于稳定。不同时期假鳞茎中可溶性糖、还原糖及淀粉的含量均高于顶叶；可溶性糖含量均先升后降，在成熟期时最高；顶叶中还原糖含量先降后升，在假鳞茎中呈升降升的变化趋势，分别在膨大期和成熟期时最高；淀粉含量均先升后降，在侧芽期时最高。Pro含量、POD活性均呈先降后升的变化趋势，在成熟期和侧芽期时较低，在膨大期和子茎期时较高。Abstract: Changes on the physiological indices including chlorophyll, relative conductivity, soluble sugars, reducing sugars, starch, proline and POD on the top-leaves and pseudo-bulbs of 3-year-old Jinhui orchid, Oncidium, at different growth stages were monitored. It was found that the chlorophyll content in the top-leaves was the lowest, while the relative conductivity highest, at the expanding stage of the plants. They stabilized subsequently. The contents of soluble sugars, reducing sugars and starch in the pseudo-bulbs were higher than those in the top-leaves. Soluble sugars in both tissue parts increased initially, then decreased, and reached a peak at maturation. The reducing sugars in the top-leaves decreased in the beginning followed with an upward trend. Whereas, in the pseudo-bulbs, the content increased at first, then decreased, and followed by an increase, with two separate peaks occurred at the expanding and maturation stages. Starch in the top-leaves and the pseudo-bulbs increased at first, maximized at the lateral bud stage, then declined. In the beginning of the plant growth, both proline content and POD activity decreased, but increased afterward. The POD activity was low at maturation and lateral bud stages, while high at expanding and pseudo-bulb off-shooting stages.
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Key words:
- Oncidium /
- top-leaf /
- pseudo-bulb /
- physiological indices /
- dynamic change
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图 1 文心兰茎叶不同生育期
注：Ⅰ为膨大期；Ⅱ为成熟期；Ⅲ为侧芽期；Ⅳ为子茎期。
Figure 1. Growth periods of Oncidium pseudo-bulbs and top-leaves
图 2 文心兰茎叶可溶性糖含量的变化
注：图中不同小写字母表示差异达显著水平(P＜0.05)，下同。
Figure 2. Changes on soluble sugar content in Oncidium pseudo-bulbs and top-leaves
图 3 文心兰茎叶还原糖含量的变化
Figure 3. Changes on reducing sugar content in Oncidium pseudo-bulbs and top-leaves
图 4 文心兰茎叶淀粉含量的变化
Figure 4. Changes on starch content in Oncidium pseudo-bulbs and top-leaves
图 5 文心兰茎叶Pro含量的变化
Figure 5. Changes on proline content in Oncidium pseudo-bulbs and top-leaves
图 6 文心兰茎叶POD活性的变化
Figure 6. Changes on POD activity in Oncidium pseudo-bulbs and top-leaves
表 1 文心兰顶叶叶绿素含量的变化
Table 1. Changes on chlorophyll content in Oncidium top-leaves
时期 叶绿素含量/(mg·g-1) 叶绿素a 叶绿素b 叶绿素(a+b) Ⅰ 0.458±0.0125 0.146±0.0038 0.604±0.0162 a Ⅱ 0.674±0.0333 0.203±0.0061 0.877±0.0393 b Ⅲ 0.644±0.0158 0.194±0.0054 0.838±0.0212 b Ⅳ 0.644±0.0452 0.222±0.0153 0.866±0.0604 b 注：不同字母表示差异达显著水平(P＜0.05)，表 2同。 
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表 2 文心兰顶叶相对电导率的变化
Table 2. Changes on relative conductivity in Oncidium top-leaves
时期 相对电导率/% Ⅰ 29.590±1.6047 a Ⅱ 23.678±2.4277 b Ⅲ 22.503±1.7642 b Ⅳ 23.660±2.2415 b
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[1] 吴容仪, 戴廷恩, 庄耿彰, 等.文心兰亚族之介绍及未来展望[J].台湾花卉园艺, 2007, (11):48-53. [2] 王燕君, 张乐萍, 谭志勇, 等. 4种文心兰的光合作用特征研究[J].现代农业科技, 2015, (16):166-167. doi: 10.3969/j.issn.1007-5739.2015.16.098 [3] 勾昕, 胡薇薇, 范亚飞, 等.文心兰切花不同开放阶段花被的生理生化变化[J].热带生物学报, 2016, 7(1):70-75. http://www.cnki.com.cn/Article/CJFDTOTAL-HNNX201601013.htm [4] 田韦韦, 王彩霞, 田敏, 等.文心兰浅绿条纹突变体的生理生化及叶绿素荧光特性研究[J].西北植物学报, 2015, 35(10):2012-2017. http://www.cnki.com.cn/Article/CJFDTOTAL-DNYX201510012.htm [5] 郑妍, 左裕, 白亭玉, 等.氮磷钾对文心兰养分含量及营养生长的影响[J].北方园艺, 2014, (15):85-88. http://www.cnki.com.cn/Article/CJFDTOTAL-BFYY201415028.htm [6] 王学奎.植物生理生化实验原理和技术:第2版[M].北京:高等教育出版社, 2006. [7] 史树德, 孙亚卿, 魏磊.植物生理学实验指导[M].北京:中国林业出版社, 2011. [8] 蔡永萍.植物生理学实验指导[M].北京:中国农业大学出版社, 2014. [9] 潘瑞炽.植物生理学:第四版[M].北京:高等教育出版社, 2001. [10] 王晓立, 韩浩章, 江宇飞.香樟黄化主要生理指标变化规律研究[J].湖北农业科学, 2010, 49(3):620-622. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201003040.htm [11] 林金水, 陆銮眉, 蔡锦玲, 等.龙船花花蕾发育过程中部分生理指标的变化[J].中国农学通报, 2010, 26(24):217-220. http://www.cnki.com.cn/Article/CJFDTOTAL-ZNTB201024046.htm [12] 张艳红, 杨东霞, 孙学东.杜鹃花花芽分化期可溶性糖和叶绿素含量的变化[J], 辽东学院学报, 2007, 14(2):64-66. http://www.cnki.com.cn/Article/CJFDTOTAL-DDFZ200702003.htm [13] 陈爱葵, 韩瑞宏, 李东洋, 等.植物叶片相对电导率测定方法比较研究[J].广东教育学院学报, 2010, 30(5):88-91. http://www.cnki.com.cn/Article/CJFDTOTAL-GJXY201005018.htm [14] 赵欣欣, 贾恩吉, 于运国, 等.玉米杂交种抗旱性鉴定与选择[J].吉林农业大学学报, 2000, 22(2):56-61. http://www.cnki.com.cn/Article/CJFDTOTAL-JLNY200002011.htm [15] 孙映波, 尤毅, 朱根发, 等.干旱胁迫对文心兰抗氧化酶活性和渗透调节物质含量的影响[J].生态环境学报, 2011, 20(11):1675-1680. doi: 10.3969/j.issn.1674-5906.2011.11.015 [16] 赵江涛, 李晓峰, 李航, 等.可溶性糖在高等植物代谢调节中的生理作用[J].安徽农业科学, 2006, 34(24):6423-6425. doi: 10.3969/j.issn.0517-6611.2006.24.008 [17] 梁悦萍, 唐道城.栽培基质对郁金香鳞茎形态发育及还原糖含量的影响[J].北方园艺, 2013(11):59-61. http://www.cnki.com.cn/Article/CJFDTOTAL-BFYY201311024.htm [18] 刘锋, 张晓艳, 刘延忠, 等.马铃薯块茎还原糖含量与钾代谢关系的研究[J].中国马铃薯, 2007, 25(5):257-260. http://www.cnki.com.cn/Article/CJFDTOTAL-MLSZ200705002.htm [19] 王冬雪, 张丽莉, 石瑛.不同熟性马铃薯各生育时期功能叶生理指标变化的研究[J], 中国农学通报, 2014, 30, (3):124-128. doi: 10.11924/j.issn.1000-6850.2013-0474 -
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