Radiation-induced Mutation and Mutant Screening on Calluses of Anthurium andraeanum
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摘要: 利用60Co-γ射线对红掌‘阿拉巴马’的愈伤组织进行辐射，根据再生植株的叶色和花色变化筛选出突变体，并对突变体与亲本进行形态学、细胞学及分子水平的差异分析。结果表明：红掌‘阿拉巴马’愈伤组织的半致死剂量接近30 Gy。当辐射剂量为30 Gy时，再生植株M1代、M2代的性状一致，染色体数目与亲本一致2n=2x=30，仅有RAPD-751引物（序列：CCCACCACAC）条带与亲本有差异，并且能够稳定遗传，从而成功诱变出红掌突变体，这对提高红掌的育种效率具有重要的理论和实践意义。Abstract: Calluses from Anthurium andraeanum (Alabama) were exposed to 60Co-γ rays to induce mutation on the tissue. Based on the color changes on the leaves and flowers, resulting mutants were selected among the regenerated plants. Subsequently, the morphological, cellular and molecular properties of the selected mutants and their parents were analyzed. The half-lethal dose of 60Co γ-ray on the callus was found to be close to 30 Gy. Treated by the dosage, the mutated M1 and M2 generations showed similar traits. They had a chromosome number same as that of the parents. But, they differed from the parents in RAPD on the D-751 primer that had a sequence of CCCACCACAC. Since the mutants could consistently pass on the inherited genes to the regenerated protoplasts, theoretically, successful breeding of mutated plants from A. andraeanum by irradiation treatment could be expected in practice.
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Key words:
- Anthurium alabama /
- callus /
- 60Co γ-ray /
- radiation-induced /
- mutant
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图 1 ‘阿拉巴马’M1代叶色变异
注：A为棕红色叶；B1为嫩绿色叶; B2为深绿色叶；CK为阿拉巴马。
Figure 1. Color variations in leaves of Alabama M1 generation
图 2 ‘阿拉巴马’M1代花色变异
注：A为红色花；B1为粉色花; B2为白色花；CK为阿拉巴马。
Figure 2. Color variations in flowers of Alabama M1 generation
图 3 亲本与M2代突变体开花植株
注：A为亲本阿拉巴马，B为突变体。
Figure 3. Flowering plants of parents and mutated M2 generation
图 4 亲本与突变体根尖染色体数
注：A为阿拉巴马根尖染色体(2n=30)，B为突变体根尖染色体(2n=30)。
Figure 4. Chromosomal number on root-tip of Alabama and mutants
图 5 亲本与突变体基因组DNA的RAPD扩增结果
注：M为DLmarker 2000，1为突变体，2为阿拉巴马。
Figure 5. Differences in genomic DNA RAPD PCR between Alabama and mutants
表 1 不同辐射剂量下红掌愈伤组织的分化率
Table 1. Differentiation rate of A. andraeanum calluses treated by varied radiation dosages
辐射剂量/Gy 愈伤个数/个 分化数/个 分化率/% 0 100 97 97 20 100 85 85 30 100 26 38 40 100 0 0 
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表 2 植物学性状调查
Table 2. Botanical character questionnaires
性状 突变体 阿拉巴马 差异性(0.05) 株高/cm 50.55±1.16 52.28±0.45 0.526 冠幅/cm 49.83±2.98 50.91±0.64 0.626 叶柄长/cm 20.12±0.37 20.12±0.37 0.839 叶长/cm 20.45±0.34 21.63±0.34 0.039 叶宽/cm 12.38±0.57 12.44±0.25 0.079 花梗长/cm 24.26±0.57 24.15±0.55 0.587 佛焰苞长/cm 11.51±0.61 11.90±0.48 0.029 佛焰苞宽/cm 8.61±0.12 8.63±0.20 0.597 花序长/cm 5.49±0.13 5.60±0.08 0.284 花序直径/mm 1.01±0.03 1.02±0.18 0.699 花朵数/朵 3.6±0.17 3.62±0.14 0.487 佛焰苞颜色 白色 红色 显著 花梗颜色 浅绿 棕红 显著 叶梗颜色 浅绿 棕红 显著
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[1] ADELHEID R KUEHRILE, FURE-CHYI CHEN, NELLIE SOGⅡ.Somatic embryogensis and plant regeneration in Anthurium andraeanum hybrids[J].Plant Cell Repom, 1992, (11):438-442. http://www.academia.edu/8891324/Somatic_embryogenesis_and_plant_regeneration_in_Anthurium_andraeanum_hybrids [2] 武爱龙.凤梨和红掌的市场前景分析[J].现代园艺, 2009, (7):43-44. http://www.cnki.com.cn/Article/CJFDTOTAL-JXYA200907028.htm [3] BROERTJES C, VAN HARTEN A M.Application of mutation breeding methods[C]//A J Abbott and R R Attkin(Editors):Improving vegetatively propagated crops.London:Academic Press, 1987:335-338. [4] 李雪, 李志英, 丛汉卿, 等.红掌ANR基因克隆及其表达与佛焰苞颜色的相关性分析[J].分子植物育种, 2013, 11(6):825-830. http://www.cnki.com.cn/Article/CJFDTOTAL-FZZW201306032.htm [5] NAGATOMI S, MIYAHIRA E.Induction of flower mutation comparing with chronic and acute gamma irradiation using tissue culture techniques in Chrysanthemum morifolium ramat[J].Acta Hortculture, 2000, 508:69-74. https://www.cabdirect.org/cabdirect/abstract/20001612059 [6] 彭文君, 田丹青, 陈集双. 60Coγ射线对红掌组培材料的辐射效应研究[J].科技通报, 2012, 28(1):95-100. http://www.cnki.com.cn/Article/CJFDTOTAL-KJTB201201020.htm [7] 高明尉.世界上第一个小麦离体诱变新品种一核组8号[J].核物理动态, 1992, 9(4):4-46. http://www.cnki.com.cn/Article/CJFDTOTAL-HWDT199204015.htm [8] 杨保安, 范家霖, 张建伟, 等.辐射与组培复合育成"霞光"等14个菊花新品种[J].河南科学, 1996, 14(1):57-60. http://www.cnki.com.cn/Article/CJFDTOTAL-HNKX601.009.htm [9] 姜长阳, 宁淑香, 杨文新, 等.愈伤组织辐射诱变选育玉兰新品系[J].园艺学报, 2002, 29(5):473-476. http://www.cnki.com.cn/Article/CJFDTOTAL-YYXB200205017.htm [10] 赵月芬.菊花辐射效应及利用组织培养加速突变体稳定的研究[J].河南科学, 1996, 14(1):57-60. http://www.cnki.com.cn/Article/CJFDTOTAL-HNXT199005002.htm [11] 高键, 卢惠萍.花卉辐射诱变育种研究进展[J].安徽农业大学学报:自然科学版, 2000, 27(3):22-24. http://www.cnki.com.cn/Article/CJFDTOTAL-ANHU200003005.htm [12] WILLIAMS J G K, KUBELIK A R, KAVIK K J, et al.DNA polymorphisms amplified by arbitrary primers arr useful as genetic markers[J].Nucl Acid Res, 1990, (18):6531-6535. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC332917/ [13] WELSH J. Fingerprinting genomes using PCR with arbitrary primers[J]. Nucl Acid Res, 1990, 18:7213-7218. doi: 10.1093/nar/18.24.7213 [14] COTIAS-DE-OLIVEIRA A L P, GUEDES M L S, BARRETO E C.Chromosome numbers for Anthurium and Philodendron spp. (Araceae) occurring in Bahia, Brazil.[J]Genetics and Molecular biology, 1999, 22(2):237-242. doi: 10.1590/S1415-47571999000200018 [15] GUILL EMAUT P, DROUARD L M. Isolation of plant DNA:A fast inexpensive, and reliable method[J].Plant Molecular Biology Report, 1992, 10(1):60-65. doi: 10.1007/BF02669265 [16] 张淑红, 陈超, 杜宏薇, 等.红掌组培变异株的特异DNA片段分析[J].北方园艺, 2010, (8):134-136. http://www.cnki.com.cn/Article/CJFDTOTAL-BFYY201008056.htm -
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