Cold Resistance of Potato Varieties for Winter-planting in Guangxi
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摘要: 探究低温胁迫下不同品种马铃薯苗期叶片生理生化特性，比较广西冬种不同马铃薯品种的耐寒性，为广西冬种马铃薯品种选育提供参考。以费乌瑞它、兴佳2号、丽薯6号3个马铃薯品种为试验材料，4℃低温下进行0、3、6、12、24、48 h胁迫，测定叶绿素、相对含水量、可溶性蛋白含量、丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性。结果表明，随低温胁迫时间的延长，3个不同马铃薯品种的相对含水量、叶绿素含量持续下降，在48 h时，费乌瑞它显著低于丽薯6号、兴佳2号。3个品种的可溶性蛋白含量、SOD、CAT活性呈先下降后上升变化趋势，而POD活性呈先上升后下降的变化趋势，在低温胁迫48 h时，丽薯6号、兴佳2号的可溶性蛋白含量、SOD活性、POD活性均显著高于费乌瑞它。各品种的MDA含量在低温胁迫48 h内呈上升变化趋势，在低温胁迫48 h时，费乌瑞它MDA含量显著高于其他品种。3个马铃薯品种的抗寒性由强到弱排列为丽薯6号>兴佳2号>费乌瑞它，与广西主栽品种费乌瑞它相比，丽薯6号和兴佳2号2个新品种具有较强的抗寒性，可在南方冬作区种植与推广应用。Abstract: Effects of low temperature on the physiology and cold stress resistance of potato plants were studied for breeding and cultivation of winter-planting varieties in Guangxi. The physiological and biochemical characteristics of the leaves on 3 selected cultivars in response to low-temperature stress during seedling stage were monitored. Favorita, Xingjia No. 2, and Lishu No. 6 were selected for the study, and the contents of chlorophyll, moisture, soluble protein, and malondialdehyde (MDA), as well as the activities of superoxidedismutase (SOD), catalase (CAT), and peroxidase (POD) in the 5th leaf on a plant were determined after the plant was grown at 4℃ for 0, 3, 6, 12, 24 or 48 h. The results showed that, the leaf relative water and chlorophyll contents of the 3 varieties declined continuously under the stress; and, at 48 h, those of Favorita became significantly lower than those of Xingjia No. 2 or Lishu No. 6. The soluble protein content and the activities of SOD and CAT in the leaves of the 3 varieties decreased initially but increased afterward. In contrast, POD activity rose at first and followed with a downward trend. Under the stress for 48 h, the soluble protein content and activities of SOD and POD in the leaves of Xingjia No. 2 and Lishu No. 6 were significantly higher than those of Favorita, while MDA increased constantly during this period. MDA in Favorita was significantly higher than the other varieties. The cold resistance of the cultivars ranked in the order of Lishu No. 6, Xingjia No.2, and Favorita. Compared to Favorita, which is the major potato variety cultivated in Guangxi, Lishu No. 6 and Xingjia No. 2 appeared to be more suitable for winter-planting to extended the growing season in the region.
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Key words:
- low temperature stress /
- potato /
- physiology and biochemistry /
- Guangxi
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图 1 低温胁迫对马铃薯叶片相对含水量的影响
注：图中不同大字母表示同一品种不同胁迫时间差异显著(P＜0.05)；不同小字母表示同一胁迫时间不同品种间差异显著(P＜0.05)，下图同。
Figure 1. Effect of low temperature stress on relative water content of potato leaves
图 2 低温胁迫对马铃薯叶片叶绿素含量的影响
Figure 2. Effect of low temperature stress on chlorophyll content of potato leaves
图 3 低温胁迫对马铃薯叶片可溶性蛋白含量的影响
Figure 3. Effect of low temperature stress on soluble protein content of potato leaves
图 4 低温胁迫对马铃薯叶片SOD活性的影响
Figure 4. Effect of low temperature stress on SOD activity of potato leaves
图 5 低温胁迫对马铃薯叶片MDA含量的影响
Figure 5. Effect of low temperature stress on MDA content of potato leaves
图 6 低温胁迫对马铃薯叶片CAT活性的影响
Figure 6. Effect of low temperature stress on CAT activity of potato leaves
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[1] 辛翠花, 蔡禄, 肖欢欢, 等.低温胁迫对马铃薯幼苗相关生化指标的影响[J].广东农业科学, 2012, 22(43):19-21. http://www.cnki.com.cn/Article/CJFDTOTAL-GDNY201222010.htm [2] 周真珍, 史端甫, 李力, 等.马铃薯东农303、大西洋在低温胁迫下生理生化及光合特性的比较[J].江西农业学报, 2016, 28(5):24-27. http://www.cnki.com.cn/Article/CJFDTOTAL-JXNY201605005.htm [3] 陈珏. 低温弱光胁迫对马铃薯光合生理特性的影响[D]. 长沙: 湖南农业大学, 2011. http://cdmd.cnki.com.cn/Article/CDMD-10537-1011203573.htm [4] 程李, 程柱, 孙洁.贵州马铃薯低温冻害及减灾措施[J].现代化农业, 2014, (8):67-69. http://www.cnki.com.cn/Article/CJFDTOTAL-XDHY201408035.htm [5] 和红云. 低温胁迫对甜瓜幼苗生长及生理生化的影响[D]. 石河子: 石河子大学, 2008. http://cdmd.cnki.com.cn/Article/CDMD-10759-2008171413.htm [6] 朱政, 蒋家月, 江昌俊, 等.低温胁迫对茶树叶片SOD、可溶性蛋白和可溶性糖含量的影响[J].安徽农业大学学报, 2011, 38(1):24-26. http://www.cnki.com.cn/Article/CJFDTOTAL-ANHU201101006.htm [7] 张宪政.植物叶绿素含量测定——丙酮乙醇混合液法[J].辽宁农业科学, 1986, (3):26-28. http://www.cnki.com.cn/Article/CJFDTOTAL-LNNY198603009.htm [8] 周祖富, 黎兆安.植物生理学实验指导[M].南宁:广西大学出版社, 2005:102-117. [9] 孔祥生, 易现峰.植物生理学实验技术[M].北京:中国农业出版社, 2008:160-162. [10] 张燕, 方力, 姚照兵, 等.PEG对烟草幼苗耐低温胁迫能力的生理效应[J].西北农业学报, 2003, 12(1):63-67. http://www.cnki.com.cn/Article/CJFDTOTAL-XBNX200301016.htm [11] 王娜, 王奎玲, 刘庆华, 等.四种常绿阔叶树种的抗寒性[J].应用生态学报, 2016, 27(10):3114-3122. http://www.cnki.com.cn/Article/CJFDTOTAL-YYSB201610007.htm [12] 邵怡若, 许建新, 薛立, 等.低温胁迫时间对4种幼苗生理生化及光合特性的影响[J].生态学报, 2013, 33(14):4237-4247. http://www.cnki.com.cn/Article/CJFDTOTAL-STXB201314002.htm [13] 许楠, 孙广玉.低温锻炼后桑树幼苗光合作用和抗氧化酶对冷胁迫的响应[J].应用生态学报, 2009, 20(4):761-766. http://www.cnki.com.cn/Article/CJFDTOTAL-YYSB200904004.htm [14] 刘瑞峰, 刘卫东, 舒必超.低温胁迫对假俭草SOD酶、MDA及可溶性糖含量的影响[J].湖南林业科技, 2012, 39(6):8-10. http://www.cnki.com.cn/Article/CJFDTOTAL-HLKJ201206005.htm [15] 李倩, 王月异, 王玉祥.低温胁迫对白三叶叶绿素、可溶性糖和丙二醛等的影响[J].中国农学通报, 2016, 32(19):83-86. doi: 10.11924/j.issn.1000-6850.casb15110105 [16] 史红梅, 张海燕, 杨彬, 等.低温胁迫对高粱幼苗MDA含量、SOD和POD活性的影响[J].中国农学通报, 2015, 31(18):74-79. doi: 10.11924/j.issn.1000-6850.casb14120134 [17] 高冠, 邹雨伽, 杨再强, 等.低温寡照胁迫对设施番茄花期叶片衰老特性的影响[J].北方园艺, 2016, (5):49-55. http://www.cnki.com.cn/Article/CJFDTOTAL-BFYY201605014.htm [18] 武雁军, 刘建辉.低温胁迫对厚皮甜瓜幼苗抗寒性生理生化指标的影响[J].西北农林科技大学学报, 2007, 35(3):139-143. http://www.cnki.com.cn/Article/CJFDTOTAL-XBNY200703028.htm [19] 董爱玲. 驯化对低温胁迫下茄子幼苗生理的影响[D]. 兰州: 甘肃农业大学, 2016. [20] 段会军, 李喜焕, 吴立强, 等.低温胁迫下西瓜幼苗生理特性与冷害的关系[J].中国农学通报, 2007, 23(1):84-87. http://www.cnki.com.cn/Article/CJFDTOTAL-ZNTB200701019.htm [21] 逯明辉, 宋慧, 李晓明, 等.冷害过程中黄瓜叶片SOD、CAT和POD活性的变化[J].西北植物学报, 2005, 25(8):1570-1573. http://www.cnki.com.cn/Article/CJFDTOTAL-DNYX200508012.htm [22] 徐冉, 任旭琴.低温对辣椒叶面积及生理指标的影响[J].安徽农业科学, 2007, 35(31):9886-9887. doi: 10.3969/j.issn.0517-6611.2007.31.043 -
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