基于LC-MS技术高通量筛选产Cardiogenol C的芽胞杆菌

潘志针; 刘波; 陈峥; 朱育菁

doi:10.19303/j.issn.1008-0384.2017.05.016

基于LC-MS技术高通量筛选产Cardiogenol C的芽胞杆菌

doi: 10.19303/j.issn.1008-0384.2017.05.016

福建省农业科学院农业生物资源研究所, 福建福州 350003

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2015R1018-10

福建省科技计划项目——省属公益类科研院所基本科研专项 2015R1018-1

福建省农业科技重大专项 2015NZ0003

详细信息

作者简介:
潘志针(1985-), 男, 硕士, 主要从事生物化学和生物活性物质分析研究

通讯作者:
刘波(1957-), 男, 博士, 研究员, 主要从事微生物生物技术及农业生物药物研究(E-mail:Fzliubo@163.com)

中图分类号: Q93
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出版历程
- 收稿日期: 2016-06-08
- 修回日期: 2016-07-22
- 刊出日期: 2017-05-28

High Throughput Screening of Cardiogenol C-producing Bacillus-like Strains Based on LC-MS

Institute of Agro-biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: Cardiogenol C是目前治疗心脏疾病的一种重要药物，本研究采用LC-MS的技术高通量从51株芽胞杆菌中筛选产Cardiogenol C的菌株。利用MassHunter软件对分子特征进行提取，根据保留时间及精确分子量等信息在Metlin代谢数据库中进行快速的比对，高通量的对代谢产物进行鉴定。筛选鉴定到1株解淀粉芽胞杆菌FJAT-17934 Bacillus amyloliquefaciens产Cardiogenol C，其相对含量为1.02%，匹配度达到了92.08%，保留时间为3.651 7 min。这为微生物源的Cardiogenol C的研发奠定理论基础。
- 解淀粉芽胞杆菌FJAT-17934 /
- LC-MS /
- Cardiogenol C
Abstract: Cardiogenol C is an important medicine for treating cardiovascular diseases. To search for a biological source for the substance, a high throughput method base on LC-MS was carried out to screen the potential Bacillus-like strains. The molecular information on the target metabolites was collected using the MassHunter software. Accordingly, substances with their retention times and molecular weights that matched those on the Metlin database were identified. Bacillus amyloliquefaciens FJAT-17934 was, thus, isolated to show a high production level of cardiogenol C in its culture medium with a relative content of 1.02%. The matching score with the database for the cardiogenol C collected was 92.08%, and the retention time, 3.6517 min.
- Bacillus amyloliquefaciens FJAT-17934 /
- LC-MS /
- cardiogenol C

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图 1 解淀粉芽胞杆菌FJAT-17934 Cardiogenol C离子提取色谱

Figure 1. Extracted ion chromatogram of cardiogenol C in Bacillus amylolique faciens FJAT-17934

下载: 全尺寸图片幻灯片

图 2 解淀粉芽胞杆菌FJAT-17934中Cardiogenol C扫描质谱

Figure 2. Scan spectrum of cardiogenol C in Bacillus amylolique faciens FJAT-17934

下载: 全尺寸图片幻灯片

图 3 解淀粉芽胞杆菌FJAT-17934中Cardiogenol C分子特征提取

Figure 3. Molecular feature extraction spectrum of cardiogenol C in Bacillus amylolique faciens FJAT-17934

下载: 全尺寸图片幻灯片

表 1 供试芽胞杆菌菌株

Table 1. Bacillus-like strains for testing

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