Selenium-enriched Cultivation and Chemical Composition of Hypsizygus marmoreus
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摘要: 为了对富硒真姬菇营养价值进行评估，采用真姬菇‘白玉10号’为试验菌株，菌草24号、本地配方作为富硒培养基进行栽培，对真姬菇产量、转化率、子实体性状、含硒量及子实体成分进行研究。结果表明：硒的添加浓度、添加方式及培养料差异均会对产量、转化率及性状产生影响。在硒浓度设计范围内，本地配方可获得硒含量在0.46~52.29 mg·kg-1，是本地CK含硒量的2.7~307.6倍，24号配方可获得含硒量在1.18~84.92 mg·kg-1，是24CK的6.94~499.53倍，子实体的含硒量与硒浓度呈正相关性。成分方面，子实体含水量、灰分和粗蛋白与硒浓度呈负相关性，而硒对子实体多糖合成有促进作用，但对总糖、脂肪的合成没有明显的促进或抑制作用。Abstract: The nutritional value of mushrooms grown on selenium (Se)-enriched medium was studied. Hypsizigus marmoreus No.10 was cultivated on JUNCAO No.24, and a local formula enriched with Se. The yield, conversion rate, fruiting traits.Se content and composition of fruiting body of the mushrooms were determined. The results showed that the amount and means of Se addition as well as the substrates in the cultivation affected the yield, conversion rate and properties of the mushrooms grown on the medium. In the concentration range tested, the Se content of H. marmoreus cultivated on the local formulation increased to 0.46-52.29 mg·kg-1, 2.7-307.6 times of that of control; whereas, on JUNCAO No. 24, the mushrooms grew to contain Se 1.18-84.92 mg·kg-1, which was 6.94-499.53 times of that of control.The Se content of the fruiting bodies of the mushrooms increased with the increasing Se of the medium. But the contents of water, ash and crude protein of the mushrooms inversely correlated with the Se concentration in the substrate. The Se appeared to promote the polysaccharide formation in the fruiting body, but showed no significant effect on either sugar or fat synthesis.
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Key words:
- Hypsizigus marmoreus /
- component analysis /
- JUNCAO
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图 1 菌草24号中真姬菇菌盖、菌柄含硒量与培养基中硒含量关系
Figure 1. Correlation between Se contents in caps and stipes of H.marmoreus and Se addition in JUNCAO No. 24 medium
图 2 菌草24号配方中真姬菇菌盖、菌柄硒的富集倍数(相对培养基含量)
Figure 2. Accumulation of Se in caps and stipes of H. marmoreus grown on JUNCAO No. 24 medium (relative to Se concentration in medium)
图 3 本地配方中真姬菇菌盖、菌柄含硒量与培养基中硒含量关系
Figure 3. Correlation between Se in caps and stipes of H.marmo-reus and Se addition in a locally formulated medium
图 4 本地配方中真姬菇菌盖、菌柄硒的富集倍数(相对培养基含量)
Figure 4. Accumulation of Se in caps and stipes of H. marmoreus grown on medium of a locally formulated medium (relative to Se concentration in medium)
图 5 不同用量硒处理组中真姬菇子实体鲜样水分含量
注：n=3，*为0.05显著水平，**为0.01极显著水平。下同。
Figure 5. Moisture content of fresh fruiting bodies of H.marmoreus cultivated by different Se treatments
图 6 不同用量硒处理组真姬菇的子实体灰分含量
Figure 6. Ash content in fruiting bodies of H.marmoreus cultivated by different Se treatments
图 7 不同用量硒处理组中真姬菇子实体总糖含量
Figure 7. Total sugar content in fruiting bodies of H. marmoreus cultivated by different Se treatments
图 8 不同用量硒处理组中真姬菇子实体多糖含量
Figure 8. Crude polysaccharide content in fruiting bodies of H. marmoreus cultivated by different Se treatments
图 9 不同用量硒处理组中真姬菇子实体粗脂肪含量
Figure 9. Crude fat content in fruiting bodies of H.marmoreus cultivated by different Se treatments
图 10 不同用量硒处理组中真姬菇子实体粗蛋白含量
Figure 10. Crude protein content in fruiting bodies of H. marmoreus cultivated by different Se treatments
表 1 本地配方中不同含量硒处理对真姬菇单产、生物学效率以及出菇周期的影响
Table 1. Yield of a bag, biological efficiency and cultivation duration of H. marmoreus grown on JUNCAO No. 24 medium with varied Se additions
表 2 本地配方中不同含量硒处理对真姬菇单产、生物学效率以及出菇周期的影响
Table 2. Yield of a bag, biological efficiency and cultivation duration of H. marmoreus grown on a locally formulated medium with varied Se additions
表 3 不同含量硒处理对真姬菇数量性状的影响
Table 3. Effect of Se concentrations on quantitative traits of H. marmoreus
表 4 菌草24号配方真姬菇子实体含硒量
Table 4. The Se content of fruiting bodies of H. marmoreus grown on JUNCAO No. 24 medium
表 5 本地配方真姬菇子实体含硒量
Table 5. The Se content of fruiting bodies of H. marmoreus grown on a locally formulated medium
表 6 培养料中硒含量与真姬菇子实体中含量关系的数学模型
Table 6. Mathematic model on correlation between Se contents in culture medium and in fruit bodies of H. marmoreus grown on the medium
表 7 不同用量硒处理组中真姬菇子实体的氨基酸组成含量
Table 7. Composition of amino acids in fruiting bodies of H. marmoreus cultivated by different Se treatments
表 8 真姬菇子实体的氨基酸组成解析
Table 8. Amino acids in fruiting bodies of H. marmoreus
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