Pathogens of Root Rot Disease on Chili Peppers in Fuzhou
-
摘要: 为明确福州地区设施辣椒根腐病病原菌种类，于2015-2016年从长乐、福清、闽侯等3地主要设施辣椒栽培区采集辣椒根腐病样本90份，分离到327株病原真菌。根据来源、培养性状以及形态学特征可将这些病原菌分为7种（来源于长乐2种、福清2种、闽侯3种）。通过菌株的致病性测定以及rDNA-ITS序列分析，结果表明来源于长乐的B1、福清的B2和闽侯的B3均具有致病性，并且在系统发育上与茄腐镰孢菌（KT271766.1）同源的置信度达99%。Abstract: Ninety chili pepper samplesinfectedbyroot rot disease were collected from Changle, Fuqing and Minhou counties in Fuzhou during 2015 to 2016 to identify the causitive fungal pathogens.Three hundred and twenty-seven strains were isolated. Based upon their origins, cultural characteristics and morphologies, these isolates were classified into 7 groups including 2 from Changle, 2 from Fuqing, and 3 from Minhou. Through pathogenic and rDNA-ITS sequence analyses, B1, B2 and B3 were determined to be pathogenic and highly homologous with Fusarium solani (KT271766.1) in phylogeny at 99% degree of confidence.
-
Key words:
- chili pepper /
- root rot /
- Fusarium solani /
- pathogens /
- identification
-
图 4 病原菌的形态特征
注：a为大型分生孢子，b为小型分生孢子和产孢瓶梗。
Figure 4. Morphological characteristics of pathogenic fungus
表 1 辣椒根腐病病原菌分离结果
Table 1. Isolation of root rot pathogens in chili peppers
采集地 分离总块数 长菌块数 菌类编号 分离频率/% 长乐 150 10 A1 6.7 96 B1 64 福清 150 15 A2 10 89 B2 59.3 闽侯 150 9 A3 6 94 B3 62.7 14 C3 9.3 
下载: 导出CSV
表 2 分离物接种辣椒结果
Table 2. Chili peppers infected by separated substance
菌种编号 接种方法 接种株数 发病株数 发病率/% A1 无创伤 75 0 0 创伤 75 0 0 B1 无创伤 75 2 2.7 创伤 75 70 93.3 A2 无创伤 75 0 0 创伤 75 0 0 B2 无创伤 75 1 1.3 创伤 75 63 84 A3 无创伤 75 0 0 创伤 75 0 0 B3 无创伤 75 4 5.3 创伤 75 68 90.7 C3 无创伤 75 0 0 创伤 75 0 0 CK 无创伤 75 0 0 创伤 75 0 0
下载: 导出CSV
-
[1] LOMAS-CANO TOMÁS, BOIX-RUIZ AMALIA, CARA-GARCÍA MIGUEL, et al. Etiological and epidemiological concerns about Pepper root and lower stem rot caused by Fusarium oxysporum f. sp. radicis-capsici f. sp. Nova[J]. Phytoparasitica, 2016, 44(3): 283-293. doi: 10.1007/s12600-016-0522-5 [2] RAJUJ, ADIVAPPAR NAGARAJAPPA, JAYALAKSHMI K, et al. aluation of Chemicals and Bioagents for Managing Phytophthora Root Rot and Root-knot Nematode Disease Complex in Capsicum under Naturally Ventilated Polyhouse[J]. Journal of Pure & Applied Microbiology, 2016, 10(2):1641-1644. [3] ESFAHANI MEHDI NASR, NASEHI ABBAS, RAHMANSHIRAZI PEGAH, et al. Susceptibility assessment of bell pepper genotypes to crown and root rot disease[J]. Archives of Phytopathology & Plant Protection, 2014, 47(8):944-953. [4] EDWARD EDKONA JENANG, WONG SING KING, TECK STEPHEN LEONG CHAN, et al. Antagonistic Activities of Endophytic Bacteria against Fusarium Wilt of Black Pepper (Piper nigrum)[J]. International Journal of Agriculture & Biology, 2013, 15(2):291-296. [5] 俞大绂.中国镰刀菌属Fusarium菌种的初步名录[J].植物病理学报, 1955, (1):1-17. [6] 曹炎瑕.棚栽辣椒根腐病发生与综合防控措施[J].西北园艺, 2013, 05:36-37. doi: 10.3969/j.issn.1004-4183.2013.03.024 [7] 郝蓉蓉, 郭海东, 杨成德, 等.西藏设施辣椒根腐病病原的分离及鉴定[J].西北农业学报, 2015, 24(12):139-143. doi: 10.7606/j.issn.1004-1389.2015.12.020 [8] 刘洋, 刘长远, 赵奎华, 等.辣椒根腐病菌-尖镰孢菌生物学特性研究[J].沈阳农业大学学报, 2009, 40(3):291-295. http://www.cnki.com.cn/Article/CJFDTOTAL-SYNY200903010.htm [9] 朱辉. 设施辣椒根腐病病原菌及检测技术研究[D]. 北京: 中国农业科学院蔬菜花卉研究所, 2007. [10] 方中达.植物研究方法:第三版[M].北京:中国农业出版社, 1998. http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm [11] 刘淑娟, 陈秀蓉, 袁宏波.孜然根腐病病原分离及鉴定[J].浙江农业学报, 2012, 24(3):481-485. http://cdmd.cnki.com.cn/Article/CDMD-10733-2006125825.htm [12] BOOTH C. The genus Fusarium[M]. Beijing:Agricultural Press, 1988. [13] 魏景超.真菌鉴定手册[M].上海:上海科学出版社, 1979. http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm [14] 王拱辰, 郑重, 叶琪明, 等.常见镰刀菌鉴定指南[M].北京:中国农业科技出版社, 1996:33-42. http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm [15] 张义红, 马占清.辣椒定植后死棵防治措施[J].农村科技, 2011, 5:29. doi: 10.3969/j.issn.1674-5329.2011.01.027 [16] 李毅毅, 李波, 高宁, 等.辣椒根部病害综合防控[J].西北园艺, 2014, 01:41-42. doi: 10.3969/j.issn.1004-4183.2014.02.029 [17] 吕佩珂, 苏慧兰, 高振江, 等.中国现代蔬菜病虫原色图鉴[M].呼和浩特:远方出版社, 2008. http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm [18] 张亭亭, 刘长远, 赵奎华, 等.辽宁省辣椒土传病害镰刀菌鉴定及rDNA-ITS序列分析[J].植物保护, 2011, 37(5):101-105. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBH201105023.htm [19] 刘丽云, 刘晓林, 刘志恒, 等.辣椒根腐病菌生物学特性研究[J].沈阳农业大学学报, 2007, 38(1):54-58. http://www.cnki.com.cn/Article/CJFDTOTAL-SYNY200701011.htm [20] IKEDA K. Role of perithecia as an inoculum source for stem rot type of pepper root rot caused by Fusarium solani f.sp. piperis (teleomorph:Nectria haematococca f.sp.piperis)[J]. Journal of General Plant Pathology, 2010, 76(4):241-246. doi: 10.1007/s10327-010-0237-2 [21] PÉREZ-HERNÁNDEZ A, SERRANO-ALONSO Y, AGUILAR-PÉREZ M I, et al. Damping-off and Root Rot of Pepper Caused by Fusarium oxysporum in Almería Province, Spain[J]. Plant Disease, 2014, 98(8):1159-1160. [22] ABOELNAGA H I G, AHMED N G. Pathogenicity, Toxicity and Gibberellic Acid Content of Fusariummoniliforme Causing Root Rot and Damping Off of Pepper[J]. Plant Pathology Journal, 2007, 6(4):318-323. doi: 10.3923/ppj.2007.318.323 [23] 刘丹. 甘肃省设施辣椒镰刀菌根腐病病原鉴定及抗病种质资源筛选[D]. 兰州: 甘肃农业大学, 2016. [24] 梁力哲.辣椒种生镰刀菌及其病理学意义的研究[J].植物病理学报, 1990, 20(2):117-121. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBL199002014.htm [25] O'DONNELL K, KISTLER H C, CIGELNIK E, et al. Multiple evolutionary origins of the fugus causing panama disease of banana:concordant evidence from nuclear and mitochondrial gene genealogies[J]. Proceedings of the the National Academy of Sciences of the United States of America, 1998, 95(5):2044-2049. doi: 10.1073/pnas.95.5.2044 -
点击查看大图
图(6) / 表(2)
计量
- 文章访问数: 1535
- HTML全文浏览量: 176
- PDF下载量: 156
- 被引次数: 0
