Physiological Responses of Garcinia celebica L. Leaves Under Low-temperature Stress
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摘要: 以3年生苏拉威西凤果Garcinia celebica L.幼苗为试材，经15℃光照培养箱炼苗72 h后，分别在3、6、9℃进行低温胁迫36 h，测定不同低温下叶片的叶绿素、可溶性蛋白、可溶性糖含量及SOD（超氧化物歧化酶）、POD（过氧化物酶）的活性。结果表明:经过低温胁迫的所有幼苗均能恢复正常生长，3℃低温的小苗出现轻微脱水现象。不同温度下的生理指标有明显变化:总叶绿素、叶绿素a、叶绿素b的含量均比对照有明显下降；可溶性蛋白、可溶性糖含量在6~9℃内随温度降低而上升，在6℃时达到最高值，但二者的含量在3℃时急剧下降，并显著低于对照；SOD、POD活性均在6~9℃内随温度降低而上升。推测苏拉威西凤果在6~9℃内出现应激反应，通过可溶性蛋白、可溶性糖的积累及SOD、POD活性的提高来适应低温胁迫。Abstract: After firstly trained at 15℃ for 72 h in an illuminated incubator, the 3-year-old Garcinia celebica seedlings were placed under 3, 6, or 9℃ for 36h for the experiment to observe the physiological responses of the plants to low temperatures. The contents of chlorophyll, soluble proteins and soluble sugars, as well as the activities of SOD and POD in the leaves of the seedlings were determined. It was found that the seedlings could restore their normal growth after an exposure to 6 or 9℃.Nevertheless, there were significant physiological alternations occurred to the plants due to the treatments. The contents of total chlorophyll, chlorophyll a, and chlorophyll b in the leaves were significantly lowered as compare to control. The soluble protein or soluble sugar content increased at 6℃ and 9℃, and peaked at 6℃. On the other hand, both of thesedecreased sharply at 3℃, and became lower than control.Meanwhile, the activities of SOD and POD in the leaves increased under 6℃ and 9℃ stresses. It was concluded that G.celebica responded to the temperatureat 6℃ or 9℃ to tolerate the cold stressby accumulating soluble proteins and sugars regulating osmosisin the tissues, and at the same time, enhancing the activities of SOD and PODfor an improved adaptability to the environmental change.
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Key words:
- G.celebica /
- cold stress /
- osmoregulation substance /
- antioxidant enzyme /
- chlorophyll /
- cold resistance
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表 1 不同低温胁迫下苏拉威西凤果叶片的电导率和叶绿素含量
Table 1. Electrical conductivity and chlorophyll content in G. celebica leaves under cold stress at low temperatures
生理指标 处理低温/℃ CK 9 6 3 相对电导率/% 9.88±0.11a 13.12±0.35b 14.38±0.61c 20.44±0.81d 总叶绿素含量/(mg·g-1) 4.76±0.10a 4.68±0.08b 4.66±0.20bc 4.63±0.10c 叶绿素a含量/(mg·g-1) 3.45±0.10a 3.34±0.10b 3.27±0.08c 3.21±0.06d 叶绿素b含量/(mg·g-1) 0.97±0.02a 0.92±0.01b 0.85±0.01c 0.63±0.00d 注：同行数据后不同小写字母表示差异达显著 (P < 0.05) 水平。表 2同。 
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表 2 不同低温胁迫下苏拉威西凤果叶片渗透调节物质、丙二醛的含量及抗氧化酶活性
Table 2. Osmotic substances, malonaldehyde content and antioxidase activities in G.celebica leaves under low-temperature stresses
生理指标 处理低温/℃ CK 9 6 3 SOD/(U·g-1) 6159±51a 6674±34b 6829±66c 5792±62d POD/(U·g-1·min-1) 225±3a 300±23b 450±42c 675±28d 可溶性蛋白质含量/(mg·g-1) 142.00±0.03a 171.90±1.00b 223.00±0.07c 126.00±0.00d 可溶性糖含量/% 15.57±7.90a 22.64±5.19b 26.89±10.21c 12.74±3.13d 丙二醛含量/(mmol·g-1) 18.78±0.88a 20.03±0.00b 21.96±0.08c 23.10±0.00d
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