新型鸭呼肠孤病毒病浓缩灭活疫苗的制备及免疫原性分析

陈仕龙; 肖世峰; 程晓霞; 林锋强; 王劭; 王锦祥; 朱小丽; 陈少莺

doi:10.19303/j.issn.1008-0384.2017.011.004

新型鸭呼肠孤病毒病浓缩灭活疫苗的制备及免疫原性分析

doi: 10.19303/j.issn.1008-0384.2017.011.004

福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心, 福建福州 350013

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2014R1023-9

福建省农业科学院青年英才项目 YC2016-1

详细信息

作者简介:
陈仕龙(1979-), 男, 博士, 副研究员, 主要从事动物病毒病研究

通讯作者:
陈少莺(1962-), 女, 硕士, 研究员, 主要从事动物传染病病原与防治研究(E-mail:chensy58@163.com)

中图分类号: S858
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- 被引次数: 0
出版历程
- 收稿日期: 2017-07-15
- 修回日期: 2017-09-20
- 刊出日期: 2017-11-28

Efficacy Evaluation of a Concentrated Inactivated Vaccine against Novel Duckling Reovirus Infection

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

摘要

摘要: 为研制高效的新型鸭呼肠孤病毒病（NDRV）灭活疫苗，分别采用病毒尿囊液和浓缩后病毒尿囊液为抗原，经甲醛灭活处理后与常规油佐剂制成灭活疫苗，并进行种鸭免疫后血清学检测、雏鸭免疫保护及安全试验。结果表明，浓缩疫苗安全性好，能明显提高疫苗的免疫效果，高峰期抗体效价的免疫持续期比常规疫苗长；浓缩疫苗免疫雏鸭后的免疫保护性达100%。以上研究表明，NDRV浓缩疫苗安全、高效，在雏鸭上具有良好的免疫保护效果，具有良好的市场前景。
- 新型鸭呼肠孤病毒感染 /
- 浓缩疫苗 /
- 常规疫苗 /
- 免疫原性
Abstract: In this paper, an experimental concentrated inactivated vaccine and a conventional inactivated vaccine against Novel Duckling Reovirus (NDRV) infection were prepared. After one single-dose immunization in adult Cherry Valley ducks, the serum samples collected in a period of 13 weeks were tested for antibodies by serum neutralisation (SN) method. The protective effects against NDRV infection were observed in 3-day-old Cherry Valley ducklings and mule duckling after a single-dose immunization. The serological results showed that concentrated inactivated vaccine was much more immunogenic than the conventional inactivated vaccine, with a much higher SN antibody titers at all test time points. The concentrated inactivated vaccine was highly protective against NDRV challenge at 7-day post-vaccination with a 100% protective efficacy, as compared to 40%-60% protective efficacy for conventional inactivated vaccine. Taken together, this experimental concentrated inactivated vaccine exhibited immune efficiency much superior to conventional inactivated vaccine and might be a useful vaccine against NDRV infection.
- Novel Duck Reovirus infection /
- concentrated inactivated vaccine /
- conventional inactivated vaccine /
- efficacy evaluation

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图 1 成年樱桃谷鸭1次免疫后血清中和抗体效价

Figure 1. Serum neutralizing antibody titers of adult Cherry Valley ducks immunized with one single-dose of inactived vaccines

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图 2 雏鸭免疫后攻毒肝脏的剖检病变

注：A为对照鸭肝脏，B为浓缩疫苗免疫攻毒鸭肝脏，C为常规疫苗免疫攻毒鸭肝脏，D为攻毒对照鸭肝脏。

Figure 2. Necropsy lesions in livers of ducklings inoculated with NDRV at 7 days post-vaccination

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表 1 雏鸭免疫后的攻毒保护试验

Table 1. Protective efficacy test on ducklings immunized with inactivated vaccines

组别	雏半番鸭				雏樱桃谷鸭
组别	肝、脾出现病变的羽数	发病率/%	死亡率/%	平均体重/g	肝、脾出现病变的羽数	发病率/%	死亡率/%	平均体重/g
浓缩疫苗免疫攻毒组	0	0	0	413.33±9.85	0	0	0	497.5±9.2
常规疫苗免疫攻毒组	3	60	0	379.0±23.9^*	2	40	0	455.33±11.3^**
攻毒对照组	5	100	0	349.5±10.7^**	5	100	0	426.17±11.0^**
空白对照组	0	0	0	411.5±9.4	0	0	0	501.67±14.1
注：表示与同日龄的对照组相比差异显著(P < 0.05)，*表示与同日龄的对照组相比差异极显著(P < 0.01)。

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参考文献(7)

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