A Vasicinol-producing Bacillus-like Bacterium
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摘要: 采用液相-四级杆飞行时间质谱的方法结合Metlin数据库检索分析14株芽胞杆菌菌株发酵液的胞外成分。筛选出一株产鸭嘴花酚碱的芽胞杆菌菌株为甲基营养型芽胞杆菌Bacillus methylotrophicus FJAT-16022，其中该成分含量占其发酵液总代谢物含量的1.39%，匹配得分为95.1。表明甲基营养型芽胞杆菌芽胞杆菌Bacillus methylotrophicus FJAT-16022为鸭嘴花酚碱高产菌株，该研究为芽胞杆菌来源鸭嘴花酚碱活性物质的开发与利用提供理论依据。
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关键词:
- 液相色谱/四极杆飞行时间质谱 /
- 甲基营养型芽胞杆菌 /
- 鸭嘴花酚碱
Abstract: A vasicinol-producing strain of Bacillus methylotrophicus FJAT-16022 was screened from 14 Bacillus-like bacteria with an aid of liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF MS) combined with retrieval comparison against the mass spectrometry database, Metlin. The obtained vasicinol had a matching score of 95.1 with the database, and its relative content was up to 1.73% of the total metabolites in the cultural supernatants. It appeared that B. methylotrophicus FJAT-16022 was a high-yield strain in producing vasicinol. The result obtained paved the way for the development and utilization of vasicinol from the identified bacterium. -
图 2 甲基营养型芽胞杆菌FJAT-16022鸭嘴花酚碱离子提取色谱
Figure 2. Extracted ion chromatogram (EIC) of vasicinol in FJAT-16022
图 3 甲基营养型芽胞杆菌FJAT-16022中鸭嘴花酚碱同位素峰结果
Figure 3. Isotopic peak of vasicinol from FJAT-16022
表 1 供试芽胞杆菌菌株
Table 1. Bacillus strains undergone screening and testing
序号 菌株编号 菌株名称 1 FJAT-10012 绳索状芽胞杆菌Bacillus funiculus 2 FJAT-10013 黏琼脂芽胞杆菌Bacillus agaradhaerens 3 FJAT-10022 盐敏芽胞杆菌Bacillus halmapalus 4 FJAT-14248 马氏芽胞杆菌Bacillus macyae 5 FJAT-14249 甲醇芽胞杆菌Bacillus methanolicus 6 FJAT-16022 甲基营养型芽胞杆菌Bacillus methylotrophicus 7 FJAT-10018 土壤短芽胞杆菌Brevibacillus agri 8 FJAT-10007 软骨素类芽胞杆菌Paenibacillus chondroitinus 9 FJAT-10020 解葡聚糖类芽胞杆菌Paenibacillus glucanolyticus 10 FJAT-10026 沙地绿芽胞杆菌Viridibacillus arenosi 11 FJAT-10028 田地绿芽胞杆菌Viridibacillus arvi 12 FJAT-14266 蜥蜴纤细芽胞杆菌Gracilibacillus dipsosauri 13 FJAT-14255 耐冷嗜冷芽胞杆菌Psychrobacillus psychrotolerans 14 FJAT-2278 副短短芽胞杆菌Brevibacillus parabrevis 
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表 2 产鸭嘴花酚碱芽胞杆菌
Table 2. Vasicinol-producingBacillus strains
序号菌株名称 菌株编号 保留时间
/min匹配得分 相对含量
/%精确分子量 质核比 CAS号 1 甲基营养型芽胞杆菌 FJAT-16022 13.6141 95.1 1.39 204.0889 203.0818 5081-51-6 2 绳索状芽胞杆菌 FJAT-10012 - - 0 - - - 3 黏琼脂芽胞杆菌 FJAT-10013 - - 0 - - - 4 盐敏芽胞杆菌 FJAT-10022 - - 0 - - - 5 马氏芽胞杆菌 FJAT-14248 - - 0 - - - 6 甲醇芽胞杆菌 FJAT-14249 - - 0 - - - 7 土壤短芽胞杆菌 FJAT-10018 - - 0 - - - 8 软骨素类芽胞杆菌 FJAT-10007 - - 0 - - - 9 解葡聚糖类芽胞杆菌 FJAT-10020 - - 0 - - - 10 沙地绿芽胞杆菌 FJAT-10026 - - 0 - - - 11 田地绿芽胞杆菌 FJAT-10028 - - 0 - - - 12 蜥蜴纤细芽胞杆菌 FJAT-14266 - - 0 - - - 13 耐冷嗜冷芽胞杆菌 FJAT-14255 - - 0 - - - 14 副短短芽胞杆菌 FJAT-2278 - - 0 - - -
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