Effect of Various Treatments on Quality of Fermented Peanut Vine/Corn Stalk Silage
-
摘要: 为探讨青贮方式、混合比例及添加剂对花生秸秆和甜玉米秸秆混合青贮发酵品质的影响,设计花生秸秆和甜玉米秸秆10:0、7:3、5:5和3:7等4种混合比例青贮,采用花生秸秆表面分离的乳酸菌和3%蔗糖混合作为添加剂,调制裹包青贮,以3:7混合比例的地上式窖贮为对照进行比较。结果表明:10:0、7:3和5:5混合比例经添加剂处理后,乳酸含量显著(P < 0.05)升高,pH值、丁酸含量和氨态氮含量显著(P < 0.05)降低;添加剂处理后,10:0混合比例青贮料的干物质含量显著(P < 0.05)提高,丙酸含量显著(P < 0.05)降低,5:5混合比例的乙酸含量显著(P < 0.05)降低;无添加剂处理时青贮料采用3:7混合比例发酵品质较好,有添加剂处理时10:0比例发酵品质较好;裹包青贮方式较优于窖贮。Abstract: Varied mixing ratios of discarded peanut vines (PV) and corn stalks (CS) and/or addition of lactic acid bacteria (LAB) with 3% sucrose were studied in 2015 to determine their effects on the quality and storage of the resulting silage. The PV:CS ratios of 10:0, 7:3, 5:5 and 3:7 (on fresh weight basis) were applied. Three replicates for each treatment were used. In addition, samples of 3PV:7CS silage were stored in an above-ground cellar for comparison. After 60 d of fermentation, silage quality was analyzed. The results showed that (1) in the 10:0, 7:3 or 5:5 PV/CS silage, the lactic acid content increased significantly (P < 0.05), while the pH, butyric acid and ammonia nitrogen decreased significantly (P < 0.05) when LAB and sucrose were added; (2) in the 10:0 PV/CS silage with the added LAB and sucrose, the dry matters increased significantly (P < 0.05), and the propionic acid content significantly decreased (P < 0.05); (3) in the 5:5 PV/CS silage with added LAB and sucrose, the acetic acid significantly decreased (P < 0.05); (4) the 3:7 PV/CS silage could deliver acceptable quality without the addition of LAB and sucrose; (5) the 10:0 PV silage could be acceptable for applicationsby adding LAB and sucrose; (6) the baled silage appeared to be better than cellar storage for the fermentation.
-
Key words:
- peanut vine /
- corn stalk /
- lactic acid bacteria /
- silage
-
表 1 添加剂和混合比例对花生秸秆与甜玉米秸秆混合青贮品质的影响
Table 1. Effect of added LAB with sucrose and PV/CS mixing ratio on quality of fermented silage
混合
比例组别 干物质
/%pH值 乳酸
/%乙酸
/%丙酸
/%丁酸
/%氨态氮
/%10:0 CK 28.54±0.33b 4.64±0.23a 1.70±0.67e 2.21±0.12b 1.18±0.13c 1.25±0.24a 3.71±0.94a A 28.96±0.82a 3.98±0.11d 6.44±0.31a 2.42±0.16b 0.22±0.21d 0.25±0.01d 2.60±0.57b 7:3 CK 25.54±0.33c 4.44±0.03b 2.70±0.67d 2.17±0.23b 1.12±0.05c 0.95±0.32b 2.52±0.94b A 25.65±0.52c 4.22±0.18c 4.61±0.68c 2.25±0.19b 1.04±0.24c 0.32±0.07d 1.17±0.60d 5:5 CK 25.41±1.25c 4.38±0.14b 2.94±1.02d 2.29±0.47b 1.72±1.85b 0.56±0.09c 2.12±0.74c A 25.48±1.54c 4.17±0.05c 4.55±0.14c 2.91±0.21a 1.08±0.13c 0.27±0.14d 1.30±0.15d 3:7 CK 21.23±0.99d 4.08±0.08d 5.20±0.21b 2.75±0.12a 2.09±0.05a 0.40±0.08d 1.11±0.14d A 21.12±0.99d 4.03±0.11d 5.23±0.16b 2.92±0.05a 2.14±0.21a 0.35±0.13d 1.26±0.08d 注:CK为对照组,A为添加剂组。同列数据后不同小写字母表示差异显著 (P < 0.05) 水平。表 2同。 表 2 贮藏方式对花生秸秆与甜玉米秸秆混合青贮品质的影响
Table 2. Effect of storage methods on quality of fermented PV/CS silage
组别 干物质
/%pH值 乳酸
/%乙酸
/%丙酸
/%丁酸
/%氨态氮
/%3:7裹包组 21.12±0.99a 4.03±0.11b 5.23±0.16a 2.92±0.05 2.14±0.21b 0.35±0.13b 1.26±0.08b 3:7窖贮组 20.79±0.99b 4.32±0.04a 4.39±0.95b 2.22±0.05 4.42±0.14a 0.66±0.22a 2.76±0.11a -
[1] 武琳霞, 都晓慧, 丁小霞, 等.花生黄曲霉毒素污染预警技术研究进展[J].中国油料作物学报, 2016, 67(4):423-425. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGYW201601019.htm [2] 谢爱娟.龙岩花生高产优质栽培技术[J].福建农业科技, 2009, 5(15):35-37. http://www.cnki.com.cn/Article/CJFDTOTAL-FJNK200905026.htm [3] 刘太字, 郭孝.花生秧饲料化利用技术研究[J].中国农学通报, 2003, 19(5):17-19. http://www.cnki.com.cn/Article/CJFDTOTAL-ZNTB200305006.htm [4] 历磊.提高花生藤营养价值的调制技术研究[D].武汉:华中农业大学, 2012. http://cdmd.cnki.com.cn/Article/CDMD-10504-1012457558.htm [5] 陆小虹, 冯清泉.花生秧秸秆粉代替苜蓿草粉对兔饲养效果的初步观察[J].上海实验动物科学, 1995, 15(4):233-234. http://www.cnki.com.cn/Article/CJFDTOTAL-SHSY504.013.htm [6] 熊路, 段银庭, 陈传安, 等.花生综合利用研究进展[J].现代农业科技, 2016, (2):278-283. http://www.cnki.com.cn/Article/CJFDTOTAL-ANHE201602168.htm [7] 张浩, 张雅君, 赵黎平, 等.花生发芽过程中蛋白质功能特性变化的研究[J].南京农业大学学报, 2014, 37(4):155-163. http://www.cnki.com.cn/Article/CJFDTOTAL-NJNY201404024.htm [8] 余伯良.秸秆饲料生产技术简介[J].粮食与饲料工业, 1997, (7):24-26. http://www.cnki.com.cn/Article/CJFDTOTAL-LSYS707.013.htm [9] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业科学技术出版社, 2003:46-75. [10] VAN SOEST P J, ROBERTSOM J B, LEWIS B A. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science, 1991, 74:3583-3597. doi: 10.3168/jds.S0022-0302(91)78551-2 [11] 徐富军.饲草饲料青贮技术及使用方法[J].河南农业, 2014, (11);15-18. http://www.cnki.com.cn/Article/CJFDTOTAL-NYHN201411042.htm [12] 王成章, 王恬.饲料学[M].北京:中国农业大学出版社, 2003:122-140. [13] 吴进东.正交设计优化农作物秸秆混合青贮模式[J].中国饲料, 2008, (3):33-36. http://www.cnki.com.cn/Article/CJFDTOTAL-SLGZ200803012.htm [14] QIN M Z, SHEN Y X. Effect of Application of a Bacteria Inoculant and Wheat Bran on Fermentation Quality of Peanut Vine Ensiled Alone or with Corn Stover[J]. Journal of Integrative Agriculture, 2013, 12(3):556-560. doi: 10.1016/S2095-3119(13)60257-9 [15] OHSHIMA M, OHSHIMA Y, KIMURA E, et al.Fermentation quality of alfalfa and Italian ryegrass silages treated with previously fermented juices prepared from both the herbages[J].Animal Science Technol, 1997:68:41-44. [16] OHSHIMA M, KIMURA Y E, YOKOTA H. A method of making good quality silage from direct cut alfalfa by spraying previously fermented juice[J].Animal Feed Science Technol, 1997, 66:129-137. doi: 10.1016/S0377-8401(96)01111-X [17] OHSHIMA M, CAO L M, KIMURA E, et al. Influence of addition of fermented green juice to alfalfa ensiled at different moisture contents[J].Grass Science, 1997, 43:56-68. [18] 辛萍萍, 孙健, 王金君.裹包青贮的应用进展[J].中国奶牛, 2008, (6):24-26. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGNN200806014.htm [19] 肖克宁, 对机械化青贮裹包新技术的实践[J].草业机械化, 2004, (2):20-21. http://www.cnki.com.cn/Article/CJFDTOTAL-NCMJ200402011.htm