Effect of Laminarin on Growth and Immunity of Pearl Gentian Grouper
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摘要: 为了探究海带多糖对珍珠龙胆石斑鱼生长性能和免疫力的影响,在石斑鱼膨化颗粒配合饲料中添加0.6%海带多糖投喂平均体重(275.55±29.79)g·尾-1的珍珠龙胆石斑鱼,结合养殖场实际生产,试验组和对照组各设2口面积30 m2水泥池,每口放养约1 800尾,在封闭式循环水饲养系统饲养66 d。试验结果表明,添加海带多糖的试验组比对照组增重率提高29.03%(P < 0.05),特定生长率提高23.88%(P < 0.05),饲料系数降低15.20%(P < 0.05);试验组石斑鱼每增重1 kg的饲料成本比对照组减少1.96元,饲料成本降低了12.54%;试验组与对照组石斑鱼肌肉营养成分差异不显著(P>0.05);试验组珍珠龙胆石斑鱼免疫活性(碱性磷酸酶AKP、溶菌酶LZM、总超氧化物歧化酶T-SOD)比对照组显著提高(P < 0.05)。研究表明,海带多糖具有提高珍珠龙胆石斑鱼生长性能、增强机体免疫力的作用。Abstract: An experimentation was conducted to determine the effect of the addition of laminarin in feed on the growth, body composition and immunity of pearl gentian groupers at an aquaculture farm.Four separate 30 m2 concrete pools were used as a closed circulating water aquaculture system for the experiment. Simultaneously, approximately 1 800 groupers were placed in each of two pools for the treatment or control. The initial weight of the individual fish was (275.55±29.79) g. They were fed with either a control diet or one containing 0.6% laminarin in the pools for 66 d. At end of the feeding period, the weight gain of the groupers in the treatment group increased 29.03% (P < 0.05), the specific growth ratio increased 23.88% (P < 0.05), and the feed conversion rate decreased 15.20% (P < 0.05) over those of control, while the nutrient content in flesh of the treatment showed no significant difference as compared with control (P>0.05). For each kg weight gain on the fish, the cost of the treatment diet was 1.96 yuan less than that of control, i.e., a reduction of 12.54%. On the other hand, the activities of alkaline phosphatase, lysozyme and total superoxide dismutase enzymes of the groupers in the treatment group were significantly higher than those of control (P < 0.05). It was, thus, concluded that addition of 0.6% laminarin in the regular feed would improve the growth and enhance the immunity of pearl gentian groupers in aquaculture.
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Key words:
- laminarin /
- pearl gentian grouper /
- growth performance /
- immunity
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表 1 石斑鱼饲料营养成分
Table 1. Nutrient content of basal diet for pearl gentian groupers
(单位/%) 组别 水分 粗蛋白 粗脂肪 粗灰分 钙 总磷 试验饲料 8.01 48.41 9.66 12.37 1.89 1.64 对照饲料 7.83 47.87 9.91 11.88 1.86 1.58 表 2 海带多糖对珍珠龙胆石斑鱼生长的影响
Table 2. Effect of laminarin on growth of pearl gentian groupers
组别 放养
数量
/尾始重/
(g·尾-1)末重/
(g·尾-1)日增重
/g平均日
增重
/g增重率
/%平均增
重率
/%特定生
长率
/%饲料
系数平均
饲料
系数成活率
/%平均成
活率
/%饲料
单价/
(元·kg-1)增重饲料
成本/
(元·kg-1)试验组
1号1810 277.4 468.2 2.89 2.96±
0.08 a68.78 73.23±
4.45 a0.83±
0.04 a1.11 1.06±
0.04 a99.78 99.87±
0.09 a12.9 13.67 试验组
2号1805 258.4 459.1 3.04 77.67 1.02 99.95 对照组
1号1820 243.6 395.3 2.30 2.40±
0.10 b62.27 56.75±
5.52 b0.67±
0.05 b1.22 1.25±
0.03 b99.84 99.91±
0.07 a12.5 15.63 对照组
2号1790 322.8 488.2 2.50 51.24 1.28 99.98 注:同行数据后不同小写字母表示差异显著 (P < 0.05)。表 3、4同。 表 3 海带多糖对珍珠龙胆石斑鱼肌肉成分的影响
Table 3. Effect of laminarin on muscle nutrient content of pearl gentian groupers
组别 水分 粗蛋白 粗脂肪 粗灰分 试验组 74.12±0.82 a 21.09±0.15 a 1.27±0.12 a 1.29±0.08 a 对照组 74.47±1.02 a 20.76±0.27 a 1.13±0.09 a 1.36±0.07 a 表 4 海带多糖对珍珠龙胆石斑鱼免疫活性的影响
Table 4. Effect of laminarin on immunity of pearl gentian groupers
组别 每100mL碱性
磷酸酶AKP
/U溶菌酶LZM/
(μg·mL-1)总超氧化物歧
化酶T-SOD/
(U·mL-1)试验组 15.20±1.15 a 6.90±0.76 a 171.50±8.97 a 对照组 10.49±0.89 b 3.49±0.65 b 146.66±7.15 b -
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