蛋白质相互作用试验技术研究进展

丁晓春; 陈维信; 李雪萍

doi:10.19303/j.issn.1008-0384.2016.10.024

蛋白质相互作用试验技术研究进展

doi: 10.19303/j.issn.1008-0384.2016.10.024

丁晓春^{1, 2, 3,},
陈维信^{1, 2, 3},
李雪萍^{1, 2, 3, ,}

1.
华南农业大学园艺学院, 广东广州 510642
2.
亚热带农业生物资源保护与利用国家重点试验室, 广东广州 510642
3.
广东省果蔬保鲜重点试验室, 广东广州 510642

基金项目:

国家自然科学基金 31372112

广州市科技项目 201604020003

农业部公益性行业科研专项 201303077-1

现代农业产业技术体系建设专项资金项目 CARS-32-09A

详细信息

作者简介:
丁晓春(1991-), 女, 硕士研究生, 研究方向:果蔬贮藏与采后生理(E-mail:502583916@qq.com)

通讯作者:
李雪萍(1966-), 女, 博士, 教授, 研究方向:果蔬贮藏与采后生理(E-mail:lxp88@scau.edu.cn)

中图分类号: Q51
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- 被引次数: 0
出版历程
- 收稿日期: 2016-06-07
- 修回日期: 2016-09-16
- 刊出日期: 2016-10-01

Research Progress of Protein-protein Interaction Technology A Research Review of the Technology of Protein-protein Interaction

DING Xiao-chun^{1, 2, 3
,},
CHEN Wei-xin^{1, 2, 3},
LI Xue-ping^{1, 2, 3
, ,}

1.
College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China
2.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangzhou, Guangdong 510642, China
3.
Guangdong Provincial Key Laboratory for Postharvest Science and Technology of Fruits and Vegetables Guangzhou, Guangdong 510642, China

摘要

摘要: 大多数表型功能的形成与蛋白质和基因产生的相互作用有关。蛋白质相互作用（PPI，protein-protein interaction）是研究蛋白质功能的重要手段，近年来发展较快。该技术在预测目的蛋白功能和基因功能上发挥了重要作用。蛋白质相互作用的研究方法主要分为3类：体内试验、体外试验、模拟生物学试验。本文比较了这些试验方法各自的特点及局限性，综述了蛋白质相互作用试验技术的研究进展及其在生物学研究中的应用，并对该技术研究进行了展望。
- 蛋白质相互作用 /
- 体内试验 /
- 体外试验 /
- 模拟生物学试验
Abstract: Most phenotypic functions come from the interaction between genes and proteins. Protein-protein interaction (PPI) is an important technology that has developed rapidly in recent years, to study protein function and this technology plays a key role in predicting the function of target proteins and genes. The main research methods of PPI are divided into three categories:in vivo, in vitro and in silico. This review compares the characteristics and limitations of these methods, summarizes the progress and application of protein-protein interaction technology in biology research, and prospects this technology.
- protein-protein interaction /
- in vivo methods /
- in vitro methods /
- in silico methods

HTML全文

表 1 蛋白质相互作用的数据库及功能介绍

Table 1. The database and function of protein interaction

序号	数据库名字	功能	参考网址
1	Struct2Net	The Struct2Net server makes structure-based computational predictions of protein-protein interactions (PPIs)	http://groups.csail.mit.edu/cb/struct2net/webserver/
2	Coev2Net	Coev2Net is a general framework to predict, assess, and boost confidence in individual interactions inferred from a high-throughput experiment	http://groups.csail.mit.edu/cb/coev2net/
3	Prism Protocol	Prism Protocol is a collection of programs that can be used to predict protein-protein interactions using protein interfaces	http://groups.csail.mit.edu/cb/coev2net/
4	InterPreTS	InterPreTS uses tertiary structure to predict interactions	http://www.russell.embl.de/interprets
5	PrePPI	PrePPI predicts protein interactions using both structural and nonstructural information	http://www.russell.embl.de/interprets
6	Iwarp	Iwarp is a threading-based method to predict protein interaction from protein sequences	http://groups.csail.mit.edu/cb/iwrap/
7	PoiNet	PoiNet provides PPI filtering and network topology from different databases	http://poinet.bioinformatics.tw/
注：[55-64]

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