福州左海湖细菌群落的季节性变化特征及典范对应分析

刘兰英; 吕新; 陈丽华; 李玥仁

doi:10.19303/j.issn.1008-0384.2016.07.018

福州左海湖细菌群落的季节性变化特征及典范对应分析

doi: 10.19303/j.issn.1008-0384.2016.07.018

刘兰英^{1, 2},
吕新^{1, 2},
陈丽华^{1, 2},
李玥仁^{1, 2, ,}

1.
福建省农业科学院农业质量标准与检测技术研究所, 福建福州 350003
2.
福建省精密仪器农业测试重点实验室, 福建福州 350003

基金项目:

福建省自然科学基金项目 2011J01118

福建省科技计划项目——省属公益类科研院所基本科研专项 2015R1025-9

详细信息

作者简介:
刘兰英(1987-),女,研究实习员,研究方向:环境微生物多样性

通讯作者:
李玥仁(1966-),男,研究员,研究方向:微生物多样性与生物安全(E-mail:yuerenli@yeah.net)

中图分类号: Q938.8;X172
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- 文章访问数: 1436
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- 被引次数: 0
出版历程
- 收稿日期: 2016-03-22
- 修回日期: 2016-04-30
- 刊出日期: 2016-07-01

Seasonal Changes on and Environmental Factors Affecting Bacterial Community at Zuohai Lake in Fuzhou

LIU Lan-ying^{1, 2},
LÜ Xin^{1, 2},
CHEN Li-hua^{1, 2},
LI Yue-ren^{1, 2
, ,}

1.
Institute for Agricultural Standards and Testing Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350003, China
2.
Key Laboratory for Precision Instrument Tests in Agricultural Fields, Fuzhou, Fujian 350003, China

摘要

摘要: 为揭示福州左海湖细菌群落的季节变化及其与环境因子的关系，通过构建16S rRNA基因克隆文库对全湖水体细菌群落进行了调查，应用典范对应分析（Canonical correspondence analysis，CCA）探讨影响细菌群落季节性变化的环境因素。结果表明：左海湖细菌群落组成中β-变形菌门β-Proteobacteria和蓝细菌门Cyanobacteria为优势类群，α-变形菌门α-Proteobacteria、拟杆菌门Bacteroidetes和放线菌门Actinobacteria为亚优势类群。细菌群落结构具有明显的季节性变化特征，春、夏季Cyanobacteria为优势类群，秋、冬季β-Proteobacteria为优势类群。春季水体细菌群落结构主要受pH、叶绿素a（Chla）、透明度（SD）和总氮（TN）的影响，夏季水体受pH、Chla、SD、总磷（TP）影响较大，秋季水体受TP、Chla、TN影响较大，冬季水体则受水温（WT）、pH、SD影响较大。本研究发现，左海湖细菌群落结构的季节动态与水温及营养状况密切相关。
- 左海湖 /
- 细菌群落 /
- 16S rRNA基因克隆文库 /
- 季节变化 /
- 典范对应分析
Abstract: To determine the seasonal changes on and the environmental factors that affected the bacterial community at Zuohai Lake in Fuzhou, a survey was conducted at 4 sampling sites on the lake from February to August in 2012. A genetic library of their 16S rRNA gene clones was established. Canonical correspondence analysis (CCA) was used to examine the correlation between the bacterial diversity and the environmental factors. The results indicated that β-Proteobacteria and Cyanobacteria were the predominate lineages in the water, followed by α-Proteobacteria, Bacteroidetes, and Actinobacteria. A significant seasonal variation in composition of the bacterial community was detected. Among various categories of bacteria,Cyanobacteria appeared most dominant in the spring and summer; while β-Proteobacteria, in the autumn and winter. The results of CCA showed that the bacterial community in the water was mainly affected by pH, chlorophyll a (Chla), transparency (SD), and total nitrogen (TN) in the spring; pH, Chla, SD, and total phosphate(TP), in the summer; TP, Chla, and TN in the fall; and, water temperature, pH, and SD, in the winter. Thus, in general, the bacterial growth in the lake closely related to the temperature as well as the nutrients available in the water.
- Zuohai lake /
- bacterial community /
- 16S rRNA gene clone library /
- seasonal changes /
- canonical correspondence analysis

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图 1 左海湖不同季节细菌群落组成

Figure 1. Compositions of bacterial community in Zuohai Lake during different seasons

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图 2 左海湖四季细菌群落与环境因子间的RDA排序

①S1~S4代表左海湖4个不同的采样站位；②A代表春季，B代表夏季，C代表秋季，D代表冬季。

Figure 2. RDA biplot on bacterial community and environmental factors of Zuohai Lake in 4 seasons

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表 1 左海湖水体理化指标

Table 1. Physio-chemical properties of water at Zuohai Lake

	春季				夏季				秋季				冬季
	S1	S2	S3	S4	S1	S2	S3	S4	S1	S2	S3	S4	S1	S2	S3	S4
经度（E）	26°5.91′N	26°5.89′N	26°5.83′N	26°5.88′N	26°5.91′N	26°5.89′N	26°5.83′N	26°5.88′N	26°5.91′N	26°5.89′N	26°5.83′N	26°5.88′N	26°5.91′N	26°5.89′N	26°5.83′N	26°5.88′N
纬度（N）	119°17.18′E	119°17.01′E	119°16.95′E	119°16.91′E	119°17.18′E	119°17.01′E	119°16.95′E	119°16.91′E	119°17.18′E	119°17.01′E	119°16.95′E	119°16.91′E	119°17.18′E	119°17.01′E	119°16.95′E	119°16.91′E
pH	7.6±0.0	7.5±0.1	7.7±0.0	7.6±0.0	7.4±0.0	7.2±0.0	7.1±0.1	7.1±0.1	7.2±0.1	7.2±0.1	7.1±0.1	7.2±0.1	7.9±0.0	7.6±0.0	7.7±0.0	7.6±0.0
WT/℃	25.0±0.3	25.1±0.1	25.0±0.2	25.1±0.1	32.1±0.5	32.2±0.5	31.9±0.1	32.0±0.5	23.1±0.1	24.0±0.1	23.7±0.1	23.9±0.1	12.8±0.2	13.0±0.2	13.0±0.1	13.1±0.2
SD/cm	40±2	36±1	40±2	32±1	48±1	33±1	33±1	37±1	20±3	25±1	24±2	25±1	34±1	39±2	42±1	39±1
COD/(mg·L^-1）	8.81±0.17	10.51±0.44	8.67±0.15	8.14±0.15	8.75±0.17	10.44±0.14	8.75±0.11	8.08±0.13	8.10±0.15	9.55±0.01	8.09±0.13	7.63±0.09	8.69±0.16	10.37±0.23	8.56±0.42	8.02±0.32
TN/(mg·L^-1）	0.41±0.03	0.45±0.02	0.55±0.02	0.39±0.06	1.00±0.03	0.91±0.04	1.28±0.07	0.98±0.04	0.87±0.07	1.06±0.07	0.92±0.03	0.55±0.08	0.42±0.04	0.54±0.05	0.42±0.08	0.63±0.02
TP/(mg·L^-1）	0.037±0.004	0.036±0.001	0.043±0.005	0.034±0.001	0.052±0.001	0.039±0.000	0.049±0.005	0.036±0.004	0.036±0.004	0.031±0.007	0.027±0.002	0.036±0.003	0.023±0.001	0.028±0.005	0.024±0.002	0.038±0.002
Chla/(mg·L^-1）	0.041±0.002	0.041±0.008	0.037±0.002	0.028±0.000	0.031±0.008	0.030±0.002	0.035±0.003	0.012±0.001	0.037±0.005	0.042±0.008	0.048±0.000	0.017±0.004	0.029±0.001	0.024±0.006	0.031±0.008	0.012±0.000
注：①S1~S4代表左海湖4个不同的采样站位；②表格中的数值代表平均值±标准差（3组平行试验的标准差）。

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表 2 左海湖细菌16S rRNA基因克隆文库分析结果

Table 2. 16S rRNA gene clone library on bacteria from Zuohai Lake

样品	克隆子/个	OTUs /个	Coverage/%	H′	1/D	Evenness (E)
春季	115	34	79.1	2.85±0.08	11.4±0.01	0.56±0.02
夏季	105	46	69.5	3.30±0.06	15.6±0.00	0.60±0.01
秋季	101	43	74.3	3.29±0.06	16.7±0.00	0.61±0.01
冬季	91	46	71.4	3.52±0.05	23.2±0.00	0.64±0.01

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