Study on Total Flavonoid content in Different Parts from Different Kinds of Pigeonpea
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摘要: 合理开发利用木豆资源，研究木豆不同品种不同部位总黄酮含量。结果表明，23份木豆种质间、器官间总黄酮含量均存在极显著性差异。木豆不同品种间叶和花的总黄酮含量为2%~4%（11M010除外），荚的总黄酮含量大多分布在2%以内。以早熟、中熟、晚熟3个品种为研究材料，其叶片、荚皮、叶柄、籽粒的总黄酮含量分别为：3.25%、2.23%、0.84%、0.22%，且存在极显著差异；比较不同成熟度木豆叶的总黄酮含量，新叶（4.09%）的总黄酮含量极显著高于成熟叶（2.98%）和老叶（2.69%）。Abstract: To exploit the pigeonpea resources reasonably, the total flavonoids of the different parts were tested from kinds of pigeonpea. Results showed that, there was highly significant difference of flavonoid content in 23 kinds of pigeonpea, and in different parts of pigeonpea, respectively. The flavonoid contents in the leaves and flowers in different breeds were in the range of 2%-4% (except for 11M010), while the flavonoid contents in the pods were almost less than 2%. The total flavonoid content in leaves from 3 different maturity varieties of pigeonpea was 3.25%, 2.23% in pod skins, 0.84% in petiole and 0.22% in beans, these values were highly significant. Furtherly, the total flavonoid was highly significant higher in new leaves (4.21%) than in mature (2.98%) and old leaves (2.69%).
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Key words:
- pigeonpea /
- different breeds /
- different parts /
- total flavonoids
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图 1 3个品种叶片、叶柄、荚皮、籽粒总黄酮含量
Figure 1. Total flavonoid contents in leaves, petiole, pod skins and beans from 3 different kinds of pigeonpea
图 2 3个品种叶片不同成熟度总黄酮含量
Figure 2. The total flavonoid contents in leaves of different maturity from 3 different kinds of pigeonpea
表 1 23个木豆品种不同器官总黄酮含量及其多重比较
Table 1. Testing and Multiple comparison of total flavonoid contents in different parts of 23 kinds of the pigeonpea
品种编号 总黄酮含量/% 多重比较 花 叶（含叶柄） 荚 平均含量 5%显著水平 1%极显著水平 11M010 2.95 5.62 1.45 3.34 a A 11M040 2.43 3.74 1.85 2.81 b B 11M011 2.55 3.65 0.59 2.67 c C 11M009 2.88 3.29 0.33 2.63 cd C 11M007 3.06 3.14 0.86 2.56 d C 11M063 2.45 3.13 1.33 2.4 e D 11M033 3.29 3.03 1.58 2.36 ef DE 11M045 2.91 2.82 1.21 2.33 ef DEF 11M051 3.59 2.81 2.03 2.32 efg DEF 11M044 3.43 2.79 0.77 2.31 efgh DEF 11M002 3.56 2.68 0.41 2.3 efgh DEF 11M021 3.05 2.63 2 2.27 fghi DEFG 11M005 2.15 2.55 0.82 2.26 fghij EFG 11M023 2.96 2.55 1.1 2.23 ghij FG 11M003 2.53 2.54 1.18 2.22 ghij FG 11M024 3.24 2.52 0.75 2.21 hij FGH 11M035 2.75 2.51 1.56 2.2 ij FGH 11M026 2.53 2.43 1.7 2.17 jk GH 11M004 3.32 2.39 0.96 2.17 jk GH MM01 3.82 2.39 0.72 2.08 k H 11M058 3.43 2.29 1.48 1.86 l I 11M001 2.76 2.08 0.63 1.84 l I 11M048 2.88 2.06 0.64 1.83 l I 平均值 2.98A 2.85B 1.13C 
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表 2 木豆花叶荚总黄酮含量方差分析
Table 2. Variance analysis of total flavonoid contents in flowers,leaves and pods of pigeonpea
变异来源 平方和 自由度 均方 F 值 P值 器官间处理 147.566 2 73.783 6551.601 0 品种间处理 21.633 22 0.983 87.314 0 器官×品种 44.203 44 1.005 89.206 0 误 差 1.554 138 0.011 总变异 214.957 206
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表 3 不同部位总黄酮含量多重比较
Table 3. Multiple comparison of the total flavonoid contents between different parts of pigeonpea
不同部位 均值 5%显著水平 1%极显著水平 叶片 3.25 a A 夹皮 2.23 b B 叶柄 0.84 c C 籽粒 0.22 d D
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表 4 不同成熟度叶片总黄酮含量方差分析
Table 4. Variance analysis of total flavonoid contents in different maturity of pigeonpea leaves
变异来源 平方和 自由度 均方 F值 P值 不同成熟度处理 11.639 2 5.82 93.233 0 品种间处理 3.085 2 1.542 24.709 0 误 差 1.124 18 0.062 总变异 17.335 26
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