Analysis on Spatial Distribution of Microbes Population in the Fermentation Bed
-
摘要: 采用定点取样、平板分离的方法,分析养猪发酵床垫料微生物的结构特性。结果表明,在30个样点5次取样过程中,微生物含量总体为细菌 放线菌 真菌。细菌含量变化幅度最大[(33.72~197.23)105cfug-1],真菌含量变化相对较小[(7.87~48.64)103cfug-1],而放线菌含量变化稳定[(20.14~33.18)104cfug-1]。细菌含量类群Ⅰ(大于70.00105cfug-1)平均9.2个样点、占总样点的28.67%;类群Ⅲ(小于30.00105cfug-1)平均为8.6个样点,占总样点的30.67%;类群Ⅱ[(30.00~70.00)105cfug-1]平均为12.2个样点,所占比例最大,为40.67%。真菌类群Ⅰ(大于70.00103cfug-1)平均仅3.8个样点,所占比例最低,为12.67%;类群Ⅱ[(30.00~70.00)103cfug-1]平均为6.4个样点,所占比例为21.33%;类群Ⅲ(小于30.00103cfug-1)平均19.8个样点,所占比例高达66.00%。放线菌3种类群所占比例与真菌类似,类群Ⅰ(大于70.00104cfug-1)平均仅1.4个样点,所占比例最低,仅为4.67%;类群Ⅱ[(30.00~70.00)104cfug-1]平均8.4个样点,所占比例为28.00%;类群Ⅲ(小于30.00104cfug-1)平均20.2个样点,所占比例高达67.33%。Abstract: Spatial distribution of microbes in the fermentation bed was analyzed by sampling and culturing, the results indicated that the amount of bacterium was highest, the second was actinomycete, the amount of fungi was lowest. In all of the samples,amplitude of variation of bacterial content was the biggest with(33.72~197.23)105cfug-1, fungal content changes are relatively small with(7.87~48.64)103cfug-1, and Actinomyces content was stability with(20.14~33.18)104cfug-1. GroupⅠ(more than 70.00105cfug-1)of bacteria content was averaged 9.2 sample points, accounting for 28.67% of the total sample; group Ⅲ(less than 30.00105cfug-1)was averaged 8.6 points, accounting for 30.67% of the total sample. Group Ⅱ (30.00~70.00)105cfug-1 was averaged 12.2 points, accounted for the largest proportion of 40.67%. Fungal group I(more than 70.00103cfug-1)was averaged only 3.8 points, the lowest proportion for 12.67%, Group Ⅱ(30.00~70.00)103cfug-1 was averaged 6.4 points, the proportion was 21.33%, group Ⅲ (less than 30.00103 cfug-1) was averaged 19.8 points, a whopping 66.00%.The proportion of actinomyces was similar to type of fungi, group I (more than 70.00104cfug-1)was averaged just1.4 sample points, the lowest proportion, only 4.67%.Group Ⅱ(30.00~70.00)104cfug-1 was averaged 8.4 points and percentage 28%, Group Ⅲ(less than 30.00104cfug-1)was averaged 20.2 sample points, a whopping 67.33%.
-
Key words:
- litter in fermentation bed /
- microbes populationg /
- spatial distribution
-
[1] 毕泗伟,吴祖芳,虞耀土.16S rDNA基因文库技术分析发酵床细菌群落的多样性[J].宁波大学学报:理工版,2013,26(1):18-22. [2] 郭艳,张进良,邓昌彦,等.猪粪堆肥升温期细菌分子生态学研究[J].河南农业科学,2010,(9):74-82. [3] 李婧.发酵床不同深度垫料中微生物与温室气体的分布特征[D].南京:南京农业大学,2012. [4] 刘恩科,赵秉强,李秀英,等.不同施肥制度土壤微生物量碳氮变化及细菌群落16S rDNA V3片段PCR产物的DGGE分析[J].生态学报,2007,27(3):1079-1085. [5] ZELLES L.Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of micro-bialcommunities in soil:a review[J].Biol Fert Soils,1999,29:111-129.
点击查看大图
计量
- 文章访问数: 1261
- HTML全文浏览量: 46
- PDF下载量: 393
- 被引次数: 0