生防菌TB2对甘蔗叶片抗病相关酶活的诱导作用

梁艳琼; 唐文; 吴伟怀; 习金根; 郑肖兰; 李锐; 郑金龙; 贺春萍; 易克贤

doi:10.19303/j.issn.1008-0384.2016.06.013

生防菌TB2对甘蔗叶片抗病相关酶活的诱导作用

doi: 10.19303/j.issn.1008-0384.2016.06.013

梁艳琼¹,
唐文²,
吴伟怀^1,3,
习金根¹,
郑肖兰¹,
李锐¹,
郑金龙¹,
贺春萍^1, ,,
易克贤^1, ,

1.
中国热带农业科学院环境与植物保护研究所/农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室, 海南海口 571101;
2.
海南大学环境与植物保护学院, 海南海口 570228;
3.
农业部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地/海南省热带作物栽培生理学重点实验室/农业部儋州热带作物科学观测实验站, 海南儋州 571737

基金项目:

中央级公益性科研院所基本科研业务费专项（2015hzs1J014、2012hzs1J012、2014hzs1J012）;中国热带农业科学院橡胶研究所省部重点实验室、科学观测实验站开放课题（RRI-KLOF201506）

详细信息

作者简介:
梁艳琼(1985-),女,助理研究员,研究方向:植物病理(E-mail:yanqiongliang@126.com)

通讯作者:
贺春萍(1974-),女,硕士,研究员,研究方向:植物病理(E-mail:hechunppp@163.com);易克贤(1964-),男,博士,研究员,研究方向:分子抗性育种(E-mail:yikexian@126.com)

贺春萍(1974-),女,硕士,研究员,研究方向:植物病理(E-mail:hechunppp@163.com);易克贤(1964-),男,博士,研究员,研究方向:分子抗性育种(E-mail:yikexian@126.com)

中图分类号: S476.1
计量
- 文章访问数: 1492
- HTML全文浏览量: 49
- PDF下载量: 422
- 被引次数: 0
出版历程
- 收稿日期: 2016-03-07
- 修回日期: 2016-04-25
- 刊出日期: 2016-06-28

Induction of Disease-resisting Enzymesin Sugarcane by Biocontrol Bacterium, TB2

1.
Environment and Plant Protection Institute, CATAS/Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China;
2.
College of Environment and Plant Protection, Hainan University, Haikou, Hainan 5702283, China;
3.
Opening Project Fund of Key Laboratory of Rubber Biology and Genetic Resource Utilization, Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation & Physiology for Tropical Crops/Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Danzhou, Hainan 571737, China

摘要

摘要: 以多酚氧化酶（PPO）、过氧化物酶（POD）、超氧化物歧化酶（SOD）、苯丙氨酸解氨酶（PAL）和过氧化氢酶（CAT）等5种防御酶作为植物抗病性反应指标，研究生防菌TB2和甘蔗赤腐病病菌对甘蔗植株抗病相关酶的影响。结果表明，先接病原菌后喷施生防菌TB2、先喷施生防菌后接病原菌、病原菌处理、生防菌处理等处理后，与植物防御抗病相关的PPO、POD、SOD、PAL、CAT防御酶活性均比对照组高，表明TB2和赤腐病菌均能诱导甘蔗叶片中的防御酶活性增强；先喷施生防菌后接病原菌的防御酶活性比同期先接病原菌后喷施生防菌的高，说明TB2诱导能力强于赤腐病菌。两者共同处理的甘蔗叶片中5种防御酶活性高于单独处理。可见TB2和赤腐病菌共同诱导具有协同增效作用。
- 甘蔗 /
- 生防菌 /
- 防御酶系 /
- 诱导抗性
Abstract: Activities of polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), phenylalanine ammonia lyase (PAL), and catalase (CAT) in sugarcane leaves were determined to evaluate the efficiency of a biocontrol bacterium, TB2, in inducing these disease-resisting enzymes, as well as the correlation between the enzymes and the disease resistance of sugarcane toward red rot pathogens. Treatments, including pathogen inoculation,TB2 spraying, and TB2 sprayings before and after pathogen inoculation, were applied to examine the variations on the enzymatic activities in the leaves of the treated sugarcane plants.It was found that the enzymatic activities in the treated plants were significantly higher than those in control, indicating an induction effect byeither TB2 or the red rot pathogens. The effect was greater when TB2 was applied along with the pathogenic inoculation, especiallyif the leaves were pre-sprayed with TB2.Although the biocontrol bacterium showed a stronger induction ability than the pathogens, a synergism seemed evidentwhen both were present on sugarcane.
- sugarcane /
- biocontrol bacteria /
- defensive enzymes /
- induced resistance

HTML全文

参考文献(5)

[1]	吴伟怀,王彩霞,梁艳琼,等.11种杀菌剂对甘蔗赤腐病菌的毒力测定[J].热带农业科学,2015,35(6):37-41.
[2]	周林,程萍,喻国辉,等.枯草芽孢杆菌TR21对香蕉抗病相关酶活的诱导作用[J].中国农学通报,2011,27(2):185-190.
[3]	李世贵,吕天晓,顾金刚,等.施用木霉菌诱导黄瓜抗病性及对土壤酶活性的影响[J].中国土壤与肥料,2010,(2):75-78.
[4]	谷医林,王远宏,常若葵,等.解淀粉芽孢杆菌Lj1诱导黄瓜抗白粉病的研究[J].农药学学报,2013,15(3):293-298.
[5]	刘淑宇,于新,陈发河,等.绿色木霉菌发酵液对芒果炭疽菌胞内抗性酶活性的影响[J].果树学报,2013,30(2):285-290.