摘要:
探究春化时间对豌豆生理生化特性的影响,为人工春化豌豆选择最佳处理时间提供理论基础。选取4种基因型豌豆品种,在豌豆长出幼芽后在4℃低温下采用0、6、12、18 d的春化时间处理,采用盆栽的方式,取种植10 d的豌豆叶片测定保护酶活性及丙二醛和可溶性蛋白含量。结果表明,与对照组相比,随着春化时间的增加,豌豆叶片中保护酶(过氧化物酶、超氧化物歧化酶、过氧化氢酶)的活性均呈现先逐步上升,在春化时间12 d时达到最大,随着春化时间的继续增加,保护酶的活性下降;丙二醛含量先缓慢升高,在春化12 d后迅速升高;可溶性蛋白含量先升高,在春化12 d后变化趋势不同。在P0.05的水平上,试验结果呈现不同的显著性差异。
关键词:
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春化 /
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豌豆 /
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保护酶 /
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可溶性蛋白含量
Abstract:
Effect of the time length of vernalization on the physiological and biochemical properties of various pea plants was investigated. Four different pea varieties were vernalizedat 4℃ for 0,6,12, and 18 ds after sprouting.The pea leaves were sampled 10 ds after growth for measurements on theprotective enzyme activities, MDA, and soluble protein content. The results showed that, as compared with control, the longer the vernalization was, the higher the activities of protective enzymes (i.e., peroxidase, superoxide dismutase, and catalase) in the leaves of the plant. The enzymatic acitivities peaked with a 12 d vernalization, and began to decline afterward. During the same period of time, the MDA content in the leaves increased slowly initially followed by a rapid rise on the 12th day.The soluble proteins in leaves increased at first, but varied after 12 d vernalization. Statistically at P0.05, these results were significantly different.
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