Biodegradation of Lignite by Trichoderma pleuroticola T9
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摘要: 采用微生物降解方法, 以煤样粒度、菌液用量、煤浆浓度和生物降解时间为影响因子, 测定侧耳木霉T9对山西褐煤的生物降解作用。结果表明:各因素对褐煤生物降解率的影响大小依次为:煤样粒度 菌液用量 煤浆浓度 生物降解时间。采用正交试验, 筛选出最优组合为:煤粒度0.1mm、菌液用量10mL、煤浆浓度2%和生物降解时间14d, 对褐煤的生物降解率最高达60.00%。Abstract: Biodegradation by Trichoderma pleuroticola T9 on lignite from Shanxi province was studied aiming to optimize the process in regard to the particle size and slurry concentration of the coal, as well as the inoculum quantity and fermentation time. The results indicated that the biodegradation was affected in the order of particle size inoculum quantity concentration of coal slurry duration of fermentation. The optimized conditions to achieve the maximum degrading rate of 60% were determined to be (a) a use of lignite with a particle size of 0.1 mm, (b) a coal slurry concentration of 2%, (c) the inoculation of 10 mL of T. pleuroticola T9 fermentation broth, and (d) incubation for 14 d.
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Key words:
- Trichoderma pleuroticola /
- lignite /
- particle size /
- biodegradation rate
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[1] 徐敬尧, 张明旭. 黄孢原毛平革菌原生质体的紫外诱变育种及对义马褐煤的生物降解转化[J]. 安全与环境学报, 2015, 15(4):227-230. [2] 徐敬尧. 球红假单胞菌生物降解褐煤的条件研究[J]. 安徽理工大学学报, 2008, 28(3):46-51. [3] SILVA-STENICO M E, VENGADAJELLUM C J, Janjua H A, et al. Biodegradation of low rank coal by Trichoderma atroviride ES11[J]. Journal of Industrial Microbiology Biotechnology, 2007, (34):625-631.
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