单丛茶树内生拮抗真菌的筛选与鉴定

蔡丽; 陈荷霞; 李胜智; 傅力

doi:10.19303/j.issn.1008-0384.2016.04.011

单丛茶树内生拮抗真菌的筛选与鉴定

doi: 10.19303/j.issn.1008-0384.2016.04.011

蔡丽¹,
陈荷霞¹,
李胜智²,
傅力^3, ,

1.
新疆农业大学食品科学与药学学院, 新疆乌鲁木齐 830052;
2.
中兴大学食品暨应用生物科技系, 台湾台中 402;
3.
韩山师范学院生命科学与食品科技学院, 广东潮州 521041

基金项目:

广东省农业领域科技计划项目(2013B020419011);广东省潮州市科技计划项目(2015N05);广东省科技计划项目(2014A02020984)

详细信息

作者简介:
蔡丽(1989-),女,硕士生,研究方向:食品生物技术

通讯作者:
傅力(1964-),女,硕士,教授,研究方向:食品生物技术(E-mail:fl1990@163.com)

中图分类号: TS201.3
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- 被引次数: 0
出版历程
- 收稿日期: 2016-01-27
- 修回日期: 2016-04-05
- 刊出日期: 2016-04-28

Screening and Identifying Endophytic Fungi from Single-clump Tea Plants

CAI Li¹,
CHEN He-xia¹,
LI Sheng-zhi²,
FU Li^{3
, ,}

1.
College of Food Science and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
2.
Department of Food Science and Biotechnology, Chung Hsing Univeresity, Taizhong, Taiwan 402, China;
3.
College of Life Sciences and Food Technology, Hanshan Normal University, Chaozhou, Guangdong 521041, China

摘要

摘要: 采用双层平板法和平板对峙法分别筛选对试验细菌指示菌和植物病原菌具有抑菌效果的内生拮抗真菌, 并通过形态特征观察和18SrDNA序列测定对拮抗真菌进行鉴定。结果表明:从单丛茶树中筛选出F15GEN、F15YE和LB35JING这3株内生拮抗真菌, 其中F15GEN对金黄色葡萄球菌抑菌圈直径为13.36mm, F15YE对枯草芽孢杆菌的抑菌圈直径为14.92mm, LB35JING和F15GEN对立枯丝核病原菌和梨腐烂病原菌的生长有抑制作用, F15YE对核桃叶枯病原菌的生长有抑制作用, LB35JING对茄腐镰刀病原菌的生长有抑制作用。经形态学和18S rDNA序列鉴定:F15GEN为斜卧青霉Penicillium decumbens, F15YE和LB35JING均属于青霉属Penicillium sp.。
- 单丛茶 /
- 内生真菌 /
- 植物病原菌 /
- 抑菌 /
- 鉴定
Abstract: Endophytic fungi antagonistic against the selected bacteria and phytopathogens were first screened from the microbial samples collected from the single-clump tea plants using the double-plate and the flat-stand methods. Then, they were identified by their morphologies and 18SrDNA sequences. Three strains of the fungi, i.e., F15GEN, F15Ye, and LB35JING, were isolated. The diameter of the inhibition zone of F15GEN against Staphylococcus aureus was 13.36 mm, and that of F15YE against Bacillus subtilis 14.92 mm. In addition, LB35JING and F15GEN showed inhibition activities against Rhizoctonia solani and Valsa ambiens Pers, F15YE against Sphaerulina juglandis, and LB35JING against Fusarium. The 18S rDNA sequence of F15GEN identified it as Penicillium decumbens, while those of F15YE and LB35JING linked them to Penicillium sp.
- single-clump tea /
- endophytic fungi /
- phytopathogens /
- inhibition activity /
- identification

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参考文献(4)

[1]	张敏, 黄亚辉, 阙伟. 做青工艺对单丛茶品质的影响[J]. 中国茶叶加工, 2012, (2):15-19.
[2]	陈汉林. 国家茶树良种岭头单丛适应性和产量品质的表现[J]. 广东茶业, 2003, (1):16-17.
[3]	RODRIGUEZ R J, REDMAN R S, HENSON J M. The role of fungal symbioses in the adaptation of plants to high stress environments[J]. Mitigation and Adaptation Strategies for Global Changes, 2004, (9):261-272.
[4]	杨冠英.苍山山系茶树内生真菌的分离及其抑菌作用研究[D]. 大理:大理学院, 2012.