Effect of Organic Solvents on Activity of N-Acetyl-β-D-glucosaminidase from Integument of Fenneropenaeus chinensis
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摘要: 以中国明对虾体壁N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)为研究对象,探讨10种有机溶剂对酶活力的影响。结果表明:甲醇、乙醇、正丙醇、乙二醇、丙三醇、二甲亚砜、二氧六环和丙酮对NAGase的作用均表现为低体积分数时有一定的激活作用,高体积分数时转化为抑制作用;而正丁醇在0%~55%范围对NAGase表现为激活作用;甲醛对NAGase的抑制作用则呈现明显的浓度效应。进一步研究甲醛和丙酮对NAGase的作用机理,结果显示:甲醛对NAGase的抑制作用是一个可逆过程,而低体积分数丙酮对NAGase的激活作用和高体积分数丙酮对NAGase的抑制作用均属于可逆过程。说明不同有机溶剂对NAGase活力有不同的影响,NAGase在不同体积分数有机溶剂中的催化作用存在差异性。
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关键词:
- 中国明对虾 /
- 有机溶剂 /
- N-乙酰-β-D-氨基葡萄糖苷酶 /
- 酶活力 /
- 体壁
Abstract: Effects of 10 organic solvents on the activity of N-Acetyl-β-D-glucosaminidase(NAGase)from the integument of Fenneropenaeus chinensis were investigated.The results indicated that the enzyme was activated in methanol,ethanol,propanol,glycol,glycerol,dimethyl sulfoxide,dioxane,and acetone in low concentrations,but inhibited in high concentrations.It was also activated in 0%to 55% butanol.The enzymatic activity was apparently formaldehyde-concentration dependent.A further study on formaldehyde and acetone showed that the inhibition by either solvent was reversible in high concentrations,and the activation,in acetone in low concentrations.The results demonstrated the varying effects of the organic solvents in different concentrations on the activity of NAGase.-
Key words:
- Fenneropenaeus chinensis /
- organic solvent /
- N-Acetyl-β-D-glucosaminidase /
- enzyme activity /
- integument
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