Crossbreeding of Different Geographical Populations of Stichopus japonicus
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摘要: 为探索刺参群体间遗传差异及其差异性与遗传距离间的关系及杂交育种的实际可行性,对不同地理群体刺参进行杂交育种试验,观察比较其育种效果,同时研究了杂交组合的生长性状。结果表明,各杂交组合均能获得正常的杂交后代,虽然相较自交组合有较低的杂交受精率,但与其他组合受精率之间并没有表现出显著性的差异;此外,研究发现不同杂交组合有不同的杂交生长优势,并且不同遗传距离的杂交组合呈现出了一定的生长规律,遗传距离在0.285 3~0.423 8范围内的6个杂交组合,总体呈现出两端大,中间小的杂交生长特性,遗传距离在0.4以上的F组合的生长速度最快,而距离靠近F组合的E组合虽然开始时生长最慢,但随着时间的延长,其生长速度表现出逐渐增大的趋势。各杂交组合遗传距离与生长相关性没有表现出十分显著的差异性。Abstract: For exploring the genetic differences between groups and the relationship between diversity and genetic distance of Stichopus japonicas,crossbreeding among different populations of Stichopus japonicas were conducted to determine the crossbreeding feasibility.The growing situation of hybrid progeny were observed.The results showed that all the hybrid combinations could get normal hybrid offspring.The fertilization rate was lower compared with combination of inbred with no significant difference;In addition,different hybrid growth advantages were detected in different crossing populations which varied with genetic distance.The crossing offspring showed bigger at both ends and smaller in the middle in six parent combination the genetic distance of which were in the range of 0.285 3~0.423 8.The combination F with genetic distance greater than 0.4 grew fastest.The crossbreeding offspring of combination E which had near genetic distance with combination F,grew slowest at the beginning,and grew faster with times.There were no significant correlation between the genetic distance of hybrid combination and growth characteristics.
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Key words:
- Stichopus japonicas /
- crossbreeding /
- fertilization rate /
- growth
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