Effects of Different Nitrogen Regimes on Soil Physico-chemical Properties and Metabolites in Rice Rhizosphere
-
摘要: 以水稻‘金恢809’为材料,探讨不同氮肥运筹方式对水稻产量、土壤酶活性、土壤理化指标以及土壤代谢物质的影响。结果表明:适当地前氮后移能显著增加水稻产量,提高土壤脲酶和蔗糖酶的活性,增加水稻生育后期土壤速效养分,降低土壤pH值。进一步采用GC-MS分析不同氮肥运筹下水稻根际土壤代谢物质的变化,发现水稻根际土壤响应氮肥调控出现变化的代谢物质主要有7大类(20种物质),主要分为醇类、酚酸类、酚类、醚类、酸酐、脂类、酸类以及酮类。综上可见,适当前氮后移有利于维护土壤碳氮代谢协调发展,促进水稻根系分泌有机酸,提高根际土壤脲酶、蔗糖酶等营养循环相关酶活性,增强土壤养分有效性,促进水稻对养分的吸收,实现水稻高产高效的目的。Abstract: The goal of this study is to understand how nitrogen(N)influences the yield,physico-chemical properties and soil enzyme activities and metabolites in rice rhizosphere by keeping the total N supply constant and varying the ratio of early to late growth stage fertilizer application.The results showed that soil urease,soil sucrose,available nutrients and yield were significantly higher in NP(efficient nitrogen application)than in NT(traditional nitrogen application).However,pH value showed the opposite trend.Furthermore,metabolomics analysis by GC-MS identified 7kinds of differentiallyregulated metabolites in soils,including alcohols,phenolic acids,phenols,ethers,anhydride and ketones.In conclusion,efficient nitrogen application can improve soil enzymes,soil available nutrients and yield.
-
Key words:
- nitrogen /
- rice /
- soil nutrient /
- soil enzyme activity /
- metabolomics
-
[1] 程式华,李建.现代中国水稻[M].北京:金盾出版社,2007. [2] 彭少兵,黄见良,钟旭华,等.提高中国稻田氮肥利用率的研究策略[J].中国农业科学,2002,35(9):1095-1103. [3] 魏海燕,张洪程,张胜飞,等.不同氮利用效率水稻基因型的根系形态与生理指标的研究[J].作物学报,2008,34(3):429-436. [4] 张志兴,陈军,李忠,等.水稻籽粒灌浆过程中蛋白质表达特性及其对氮肥运筹的响应[J].生态学报,2012,32(10):3209-3224. [5] 张福锁,王激清,张卫峰,等.中国主要粮食作物肥料利用率现状与提高途径[J].土壤学报,2008(05):915-924. [6] 刘建军,陈海滨,田呈明,等.秦岭火地塘林区主要树种根际微生态系统土壤性状研究[J].土壤侵蚀与水土保持学报,1998(03):53-57. [7] 伍俊艺,王鸿斌,姜亦梅.不同耕作对黑土酶活性变化特征的影响研究[J].中国农学通报,2012(36):124-127. [8] WECKWERTH W,WENZEL K,FIEHN O.Process for the integrated extraction,identification and quantification of metabolites,proteins and RNA to reveal their co-regulation in biochemical networks[J].Proteomics.2004,4(1):78-83. [9] 李忠,陈军,林世圣,等.氮肥运筹比例对水稻生长及产量的影响[J].福建农业学报,2011(04):557-561. [10] 陈林,张佳宝,赵炳梓,等.不同施氮水平下土壤的生化性质对干湿交替的响应[J].土壤学报,2013(04):675-683. [11] 蒋朝晖,曾清如,皮荷杰,等.不同品种尿素施入土壤后pH值的变化和氨气释放差异[J].环境化学,2009(02):177-180. [12] ALLISON S D,CZIMCZIK C I,TRESEDER K K.Microbial Activity And Soil Respiration Under Nitrogen Addition In Alaskan Boreal Forest[J].Global Change Biology.2008,14(5):1156-1168. [13] 陈林,张佳宝,赵炳梓,等.不同施氮水平下土壤的生化性质对干湿交替的响应[J].土壤学报,2013(04):675-683. [14] STARK C H,CONDRON L M,O CALLAGHAN M,et al.Differences in soil enzyme activities,microbial community structure and short-term nitrogen mineralisation resulting from farm management history and organic matter amendments[J].Soil Biology&Biochemistry.2008,40(6):1352-1363. [15] JANGID K,WILLIAMS M A,FRANZLUEBBERS A J,et al.Relative impacts of land-use,management intensity and fertilization upon soil microbial community structure in agricultural systems[J].Soil Biology&Biochemistry,2008,40(11):2843-2853. [16] WANG Q K,WANG S L,LIU Y X.Responses to N and P fertilization in a young Eucalyptus dunnii plantation:Microbial properties,enzyme activities and dissolved organic matter[J].Applied Soil Ecology,2008,40(3):484-490. [17] 吕卫光,沈其荣,余廷园,等.酚酸化合物对土壤酶活性和土壤养分的影响[J].植物营养与肥料学报,2006(06):845-849. [18] 马云华,王秀峰,魏珉,等.黄瓜连作土壤酚酸类物质积累对土壤微生物和酶活性的影响[J].应用生态学报,2005(11):145-149. [19] 袁光林,马瑞霞,刘秀芬,等.化感物质对土壤脲酶活性的影响[J].环境科学,1998(02):57-59. [20] 朱静平,程凯.3种水培植物根系分泌的有机酸对氮循环菌的影响[J].环境工程学报,2011(09):2139-2143.
点击查看大图
计量
- 文章访问数: 177
- HTML全文浏览量: 44
- PDF下载量: 2
- 被引次数: 0