美丽短芽胞杆菌Brevibacillus formosus FJAT-10011抑菌效应及其产蛋白酶条件优化
doi: 10.19303/j.issn.1008-0384.2015.10.008
Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011
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摘要: 对筛选到产蛋白酶且抑菌的美丽短芽胞杆菌Brevibacillus formosus FJAT-10011通过单因素和正交试验对该菌株发酵条件进行优化。优化培养基组成:可溶性淀粉1.25%、蛋白胨1.25%、牛肉浸粉0.3%、NaCl0.5%、硫酸镁0.05%、磷酸氢二钾0.05%。优化发酵条件为:发酵初始pH 5.5、发酵温度25℃、发酵培养基装液量30mL/250mL、种子液接种量1%、发酵时间24h。在优化条件下,上清液酶活力达到8.41U·mL-1,是初始培养条件的141倍。抑菌直径为15.71mm,比未优化的提高35.31%。Abstract: Antibacterial efficacy andprotease-producing capability of Brevibacillus formosus FJAT-10011 were studied.Screening and optimizing the culture conditions were conducted with a single-factor test and an orthogonal experiment.The optimal medium was determined to contain 1.25% starch,1.25% peptone,0.3% beef extract powder,0.5% NaCl,0.05% MgSO4,and 0.05% K2 HPO4.The optimized fermentation process included the following conditions:an initial pH of 5.5,a temperature at 25℃,the filling of the liquid medium at 30 mL/250 mL,a bacterial inoculum rate at 1%,and a fermentation duration of 24 h.Under these conditions,the protease activity of the supernatant of the liquid medium reached 8.41U·mL-1,which was 141 times greater than that in the beginning of the fermentation.The diameter of the antibacterial zone shown on a petri dish test was 15.71 mm,a 35.31%increase as compared to the non-optimized control.
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Key words:
- protease /
- antibacterial efficacy /
- fermentation optimization /
- single factor /
- orthogonal test
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[1] 董秋月,陈檬燕,辛杨,等.益生菌的筛选及安全性研究进展[J].食品研究与开发,2012,33(10):228-231. [2] 张娟,杨彩梅,曹广添,等.解淀粉芽胞杆菌及其作为益生菌的应用[J].动物营养学报2014,26(4):863-886. [3] IBRAHIM A N,JONATHAN P O,BEN C M,et al.Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs[J].Animal Feed Science and Technology,2015,201:57-65. [4] ROMERO L F,PARSONS C M,UTTERBACK P L,et al.Comparative effects of dietary carbohydrases without or with protease on the ileal digestibility of energy and amino acids and AMEn in young broilers[J].Animal Feed Science and Technology,2013,181(1):35-44. [5] SHEA C J O,MC ALPINE P O,SOLAN P,et al.The effect of protease and xylanase enzymes on growth performance,nutrient digestibility,and manure odour in grower-finisher pigs[J].Animal Feed Science and Technology,2014,189:88-97. [6] 刘燕.猪益生性芽袍杆菌的筛选与特性研究[D].北京:中国农业科学院,2006. [7] 孟小亮,陈昌福,高宇,等.1株黄颡鱼肠道益生菌的筛选与鉴定[J].华中农业大学学报,2010,29(2):208-212. [8] 胥海东,王永泽,王金华.枯草芽胞杆菌产蛋白酶的发酵培养基优化[J].食品与发酵科技,2011,47(4):77-81. [9] 文凤云,王国永,李晓丽,等.多粘芽胞杆菌Dw-6菌株培养条件的优化及其发酵液抑菌活性的初步研究[J].西北农业学报,2011,20(4):30-34. [10] 奚家勤,冯云利,薛超群,等.烤烟品种K326内生细菌分离、抗黑胫病菌株筛选及种群组成分析[J].烟草科技,2013,(3):77-82. [11] 车建美,郭慧慧,刘波,等.产挥发性物质芽胞杆菌对枇杷炭疽病菌的抑制作用及其鉴定[J].福建农业学报,2014,29(5):469-474. [12] 付博,李炎琪,李忠玲,等.一株拮抗猕猴桃叶枯病的美丽短芽胞杆菌NF2的He?Ne激光诱变育种[J].西北大学学报,2014,44(1):66-70. [13] 程仕伟,王梦龙,冯志彬,等.碱性蛋白酶产生菌的筛选鉴定及培养优化[J].食品与生物技术,2012,31(12):1314-1319. [14] SELMIHAN S,ISMAIL O,ESENGUL K.Purication,immobilization,and characterization of protease from local Bacillus subtilis M-11[J].Asia-Pacic Journal of Chemical Engineering,2015,10:241-247. [15] BIZANI D,BRANDELLI A.Characterization of a bacteriocin produced by a newly isolated Bacillus sp.Strain 8 A[J].Journal of the Korean Society for Applied Biological Chemistry,2014,57(5):605-612. [16] 郝晓娟,刘波,葛慈斌,等.短短芽胞杆菌JK-2菌株抑菌活性物质产生条件的优化[J].河北农业科学,2009,13(6):46-49. [17] 刘文涛,刘柏宏,张娟,等.枯草芽胞杆菌产角蛋白酶的发酵过程优化[J].食品与生物技术学报,2014,33(5):466-471. [18] 王霁宁,严孝强,杜宗军.琼胶酶高产海洋细菌QM42的发酵条件优化[J].中国酿造,2012,31(7):86-90. [19] 赵东洋.解淀粉芽胞杆菌SWB16脂肽类代谢产物对球孢白僵菌的拮抗作用及发酵条件的初步优化[D].重庆:西南大学,2014. [20] 杨永青,谢远红,张红星,等.枯草芽胞杆菌C3产抗菌物质发酵条件优化[J].中国酿造,2014,33(3):28-31. [21] 安文静.侧孢芽胞杆菌BL-1产抗菌物质发酵条件的研究以及抗菌物质的分离纯化[D].哈尔滨:黑龙江大学,2008. [22] 朱泓,王一明,林先贵.一株高温蛋白酶高产菌株产酶条件的优化[J].南京林业大学学报,2014,38(1):31-35. [23] SEYED G S,BASERISALEHI M,BAHADOR N.Study on Bioactive Compounds Produced by Soil Origin Brevibacillus spp.[J].Nature Environment and Pollution Technology,2013,12(2):209-214. [24] SAVITA M,GOTHWAL R K,MOHAN M K,et al.Purnendu Ghosh Production and purification of a hyperthermostable chitinase from Brevi Brevibacillus formosusBISR-1isolated from the Great Indian Desert soils[J].Extremophiles,2014,18:451-462.
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