Aseptic Sowing and Rapid Propagation of Cymbidium ensifolium var.susin
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摘要: 以素心建兰种子为外植体,通过基本培养基(1/2MS、花宝1号、改良MS)、植物激素(6-BA、TDZ、NAA)等关键因子对素心建兰种子萌发、根状茎增殖、分化等各培养阶段影响的试验,探讨了素心建兰无菌播种快繁关键技术。结果表明:种子无菌萌发较适宜的培养基配方为1/2MS+6-BA3.0 mg·L-1+NAA 0.5mg·L-1+水解乳蛋白2.0g·L-1+活性碳1.0g·L-1+蔗糖30g·L-1;根状茎较适宜的增殖培养基配方为改良1号+TDZ 1.0mg·L-1+NAA 0.5mg·L-1+活性碳0.5g·L-1+白糖30g·L-1,45d增殖系数高达7.3;根状茎接种于改良1号+6-BA 2.0mg·L-1+NAA 0.1mg·L-1+白糖30g·L-1培养基上分化培养45d后,再转入1/2MS+IBA 0.5mg·L-1+活性碳0.5g·L-1+白糖20g·L-1培养基上培养60d,可诱导形成完整植株,芽分化率为86.3%,生根率为100.0%。Abstract: Seeds of Cymbidium ensifolium var.susin were used as the explant tissue to study the aseptic sowing and rapid propagation in situcultivation for the plant.Effects of the medium(MS,MS,Hyponex No.1or improved MS)and phytohormone(6-BA,TDZ,and/or NAA)on the mass breeding during seed germination,rhizome multiplication,and rhizome differentiation stages.The results showed that the 1/2MS medium with 6-BA 3.0mg·L-1,NAA 0.5mg·L-1,LH 2.0g·L-1,AC 1.0g·L-1 and sugar 30g·L-1 was conducive to the seed germination;the improved medium No.1with TDZ 1.0mg·L-1,NAA 0.5mg·L-1,AC 0.5g·L-1 and sugar30g·L-1 was favorable for the rhizome multiplication(the propagation coefficient reached 8.1in 45days);and,the improved medium No.1with 6-BA2.0mg·L-1,NAA 0.1mg·L-1and sugar 30g·L-1for 45 days followed by transferring the subculture onto the MS with IBA 0.5mg·L-1,AC 0.5g·L-1and sugar 20g·L-1for 60 days was bestfor rhizome differentiation.At the end,young plantlets emerged and developed into mature plants with a rhizome differentiation rate of 86.3% and a rooting rate of 100.0%.
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Key words:
- Cymbidiumensifolium /
- aseptic sowing /
- rhizome /
- rapid propagation
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