Enhancing Effect of Transferred Ferredoxin Proteins in Oncidiumon Disease-resistance of Longan
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摘要: 以含5mg·L-1潮霉素MS培养基进行抗性筛选培养,以EHA105农杆菌介异,将龙眼2个非光合铁氧还蛋白基因导入文心兰,结果表明,所获Fd3转化植株表现更高的移栽成活率和对抗病害的能力。与野生型植株相比,Fd3转化植株抵抗软腐病能力提高,由高感软腐病(Di=100)提升为中抗软腐病(Di=27.5),移栽成活率由79.3%提高至95.7%,均达极显著差异水平。Abstract: To study the enhancing effect on the disease-resistance of longan by genetic modification,twononphotosynthetic ferredoxins from the plant were transferred to Oncdiumthrough Agrobacterium tumefaciens strain EHA105 for cultivation on a culture medium containing 5mg·L-1hygromycin.As compared to the plants in the wild,the Fd3 transgenic plants showed a significantly higher resistance to soft rot disease improving the resistance grading from highly sensitive(Di=100)to moderate(Di=27.5).In addition,the survival rate of the transplants also increased significantly from 79.3%to 95.7%.
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Key words:
- cut flowers /
- Oncidium /
- transgene /
- ferredoxin /
- disease-resistance
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