Effect of Root Extracts on Growth,Pathogenicity and Efficacy of FJAT-1458 for Plant Vaccination
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摘要: 通过对番茄、辣椒、粉蕉和韭菜进行根系液浸提,研究其对青枯病疫苗菌株FJAT-1458菌体生长、致病和防效能力的影响。结果表明,不同植物根系浸提液对植物疫苗菌株FJAT-1458生长能力的影响不同。番茄、辣椒、粉蕉的根系浸提液对菌株FJAT-1458菌体生长有促进作用,番茄和辣椒对致病性青枯雷尔氏菌FJAT-91菌体生长也有促进作用,其中番茄根系浸提液对菌株FJAT-1458和FJAT-91的促进作用最明显,处理32h,FJAT-1458和FJAT-91的菌体浓度分别比对照增长了28和30倍。而韭菜对菌株FJAT-1458和FJAT-91生长具有抑制作用,处理8h,菌体浓度为0。比较不同植物根系的浸提液对FJAT-1458和FJAT-91致病性的影响,结果表明FJAT-1458和FJAT-91的菌落弱化指数随处理时间差异不显著(P>0.05),此外,不同植物根系浸提液处理的FJAT-1458对番茄青枯病防效(除韭菜的根系浸提液处理)影响也不明显。Abstract: Water extracts from the roots of tomato,chili pepper,plantain banana and chive were tested for their effects on the growth,pathogenicity and efficacy of the vaccination bacterium,FJAT-1458,in controlling Ralstonia solanacearum(FJAT-91).It was found that the root extracts exhibited varied effects on the growth characteristics of microbes.The extracts of tomato,chili pepper and plantain banana roots promoted the growth of FJAT-1458,while those of tomato and chili pepper roots enhanced the growth of both FJAT-1458 and FJAT-91.Among the extracts,the tomato extract showed a most significant growth effect on both strains,as the bacterial counts of FJAT-1458 and FJAT-91 increased 28and 30 times,respectively,as compared with control after a 32 hincubation.In contrast,the chive extract killed them in 8hours.Further study showed that the attenuation indices on FJAT-1458 and FJAT-91 were not significantly different among the various extracts.And,these root extracts,except chive,showed no significant effect on the control efficacy of FJAT-1458 toward R.solanacearum.
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Key words:
- plant root extract /
- Ralstonia solanacearum /
- plant vaccine /
- growth characteristics /
- pathogenicity /
- control efficacy
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