A Preliminary Study on Regeneration of PleurotuseryngiiProtoplast
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摘要: 为探索杏鲍菇原生质体的再生条件,探讨了再生方式、稳渗剂、培养基、菌龄及其他因素对杏鲍菇原生质体再生的影响。结果表明,5~7d菌丝制备的原生质体在以NaCl为稳渗剂的酵母粉蛋白胨再生培养基上再生率最高,可达0.9%。在制备原生质体时,低酶解温度、短酶解时间、低酶含量有利于原生质体的再生。Abstract: Effect of regenerating method,osmotic pressure stabilizer,culture medium,age of mushroom,etc.on the regeneration of Pleurotus eryngii protoplast was investigated.The results showed that the protoplasts from the 5-7days old mycelia yielded the most desirable results when cultured in a yeast peptone regeneration medium with up to 0.9% of NaCl as the osmotic stabilizer.Lowered temperature,shortened time and reduced enzyme concentration could help minimize enzymatic hydrolysis in the preparation which would benefit the protoplast regeneration.
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Key words:
- Pleurotus eryngii /
- protoplast /
- regeneration rate
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