摘要:
副溶血弧菌是一种嗜盐的革兰氏阴性菌,它广泛分布于温暖海域的海产品中,如鱼、虾、贝类等,食用被其感染的海鲜可导致食用者出现腹泻、肠痉挛、恶心、呕吐等典型胃肠炎症状,严重时可引起败血症,肾功能衰竭等临床反应。近年来,由副溶血弧菌引发的食物中毒事件的发生比例呈猛烈的上升趋势,并且领先于其他食源性致病菌,居于首位。鉴于其污染范围广,致病力强,对副溶血弧菌实现快速、灵敏、经济、有效的检测是减少其污染海洋产品,保障人体健康的关键,也是当代研究的热门。目前,基于其毒力致病机理的检测方法得到了很大的发展,主要包括分子生物学范畴的PCR法、环介导等温扩增技术、核酸探针技术、基因芯片技术等与免疫学范畴的ELISA,胶体金技术等。本文综述了在免疫学、分子生物学等方面的检测方法的研究进展及其优缺点,以期为同类研究提供参考和理论依据。
Abstract:
Vibrio parahaemolyticus(Vp)is a halophilic gram-negative bacterium.It widely exists in warm water fishes,shrimps and shellfishes.The seafood carriers,when ingested,can cause the typical gastroenteritis symptoms including diarrhea,intestinal cramps,nausea and vomiting.When a patient is seriously infected,septicemia and kidney failure may result.The rate of the food poisoning incidents caused by Vp has increased drastically in recent years,reaching the top of the list among all foodborne illnesses.With the wide-spread nature and seriousness of the diseases in humans,the need for a rapid,sensitive,low-cost and effective detection method has become apparent.Methodologies based on the virulence factors that apply the molecular biology techniques including PCR,Loopmediated isothermal amplification,nucleic acid probe and gene chip,as well as the immunology tools,such as ELISA and the immune colloidal gold,are being explored.This article evaluates the relevant methods to provide a comprehensive reference for the related studies.
点击查看大图
下载:
