模拟空间环境对朱砂根愈伤组织岩白菜素合成的影响

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基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1017-4)

福建省种业创新与产业化工程项目(2014S1477-2)

福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT-I-0305)

福建省农业科学院青年人才创新基金(2014CX-18)

Clinostat and UV Treatments for Bergenin Synthesis in Callus of Ardisia crenata Sims.

1. Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2. Fuzhou Scientific Observing and Experimental Station of Agro-Environment, MOA, Fuzhou, Fujian 350013, China

摘要: 利用自行研制的旋转装置和紫外灯管辐射培养朱砂根的愈伤组织,研究模拟空间环境对愈伤组织岩白菜素合成的影响,以期寻找到有利于岩白菜素合成和积累的环境因子。研究结果表明:经旋转处理的朱砂根愈伤组织岩白菜素含量明显提高,且峰值出现的时间比对照提前,峰值是对照的1.85倍;波长为308nm的紫外线辐射对愈伤组织培养前期的岩白菜素积累有利。本研究明确了2种模拟空间环境对朱砂根愈伤组织岩白菜素合成有不同的促进作用,但对愈伤组织的生长都有抑制作用,将为朱砂根愈伤组织岩白菜素的进一步利用奠定基础。
- 模拟微重力 /
- 紫外辐射 /
- 朱砂根 /
- 岩白菜素
Abstract: A lab-designed clinostat and a UV lamp were applied to enhance the synthesis and accumulation of bergenin in the culture of Ardisia crenata Sims.calli.The simulated microgravity significantly increased the bergenin content with a peak amount 1.85 times of that of control.It also hastened the bergenin formation shortening the time to reach its peak in the calli.The exposure of calli to UV at 308 nm during the early stage of the culture benefited the bergenin accumulation.Both treatments appeared to induce varied effects in promoting the bergenin synthesis and accumulation.However,at the same time,they also retarded the callus growth.
- simulated microgravity /
- UV /
- Ardisia crenata Sims. /
- bergenin