Identification of Pathogen Responsible for White-spots Disease in Larimichthys crocea
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摘要: 从福建三都澳网箱养殖患病的大黄鱼脾、肾分离到2株优势菌(H2013032002和H2013032003),通过人工感染试验证实其为大黄鱼内脏白点病病原,应用API-20E生理生化和16SrRNA分子系统鉴定,药物敏感试验筛选敏感药物。生化鉴定结果显示2株菌与恶臭假单胞菌和荧光假单胞菌的符合度为75.2%;16SrRNA序列分析结果表明:该2株菌与恶臭假单胞菌同源率高达99.0%,确定这2株菌为恶臭假单胞菌;药物敏感试验表明:病原菌对诺氟沙星、环丙沙星、恶喹酸、卡那霉素等4种药物高度敏感。Abstract: Two strains of the suspected bacteria,H2013032002 and H2013032003,were isolated from croakers,Larimichthys crocea,in Sanduao,Fujian.An artificial infection with the isolates caused the similar white-spots disease in the fish.Subsequently,morphological and biochemical tests identified the isolated strains as Pseudomonas putida or P.fluorescent.Then,the 16S-rDNA sequence analysis showed that the bacteria had a 99.0%homologous rate with P.putida.In addition,a drug sensitivity test revealed that the two strains of pathogen were highly sensitive to norfloxacin,ciprofloxacin,oxolinic acid and kanamycin neomycin.It was,therefore,determined that P.putid was likely the culprit that caused the white-spots disease in croakers.
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Key words:
- Larimichthys crocea /
- white-spots disease /
- Pseudomonas putida /
- isolation and identification /
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