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生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化

曾庆才, 肖荣凤, 刘波, 陈燕萍, 史怀

曾庆才, 肖荣凤, 刘波, 陈燕萍, 史怀. 生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化[J]. 福建农业学报, 2015, 30(2): 192-197. DOI: 10.19303/j.issn.1008-0384.2015.02.018
引用本文: 曾庆才, 肖荣凤, 刘波, 陈燕萍, 史怀. 生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化[J]. 福建农业学报, 2015, 30(2): 192-197. DOI: 10.19303/j.issn.1008-0384.2015.02.018
CENG Qing-cai, XIAO Rong-feng, LIU Bo, CHEN Yan-ping, SHI Huai. Optimization of Solid-state Fermentation Condition for the Biocontrol Strain of Trichoderma harzianum FJAT-9040 by Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 192-197. DOI: 10.19303/j.issn.1008-0384.2015.02.018
Citation: CENG Qing-cai, XIAO Rong-feng, LIU Bo, CHEN Yan-ping, SHI Huai. Optimization of Solid-state Fermentation Condition for the Biocontrol Strain of Trichoderma harzianum FJAT-9040 by Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 192-197. DOI: 10.19303/j.issn.1008-0384.2015.02.018

生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化

基金项目: 

国家农业成果转化项目(闽财指[2014]969号)

福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1018-5)

福建省发改委项目——闽发改高技[2013]577号

详细信息
    作者简介:

    曾庆才(1987-),男,硕士,研究方向:应用微生物学;刘波(1957-),男,博士,研究员,研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

  • 中图分类号: S476

Optimization of Solid-state Fermentation Condition for the Biocontrol Strain of Trichoderma harzianum FJAT-9040 by Response Surface Methodology

Funds: 

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福建省农业科学院农业生物资源研究所,福建 福州,350003

  • 摘要: 为优化生防菌哈茨木霉FJAT-9040固体发酵条件,结合初始含水量、初始接种量及培养温度3个单因素,Box-Behnken设计和响应面分析法进行研究。以产孢量为指标,单因素试验确定初始含水量、初始接种量及培养温度分别为50%、5%、28℃。响应面分析结果表明:初始含水量和温度与产孢量有显著相关性(P<0.05),通过求解回归方程可得,当初始含水量为53%,初始接种量为7%,培养温度29℃时,产孢量达到4.04×109个·g-1,与实际值3.86×109个·g-1相近。
    Abstract: Optimization of solid-state fermentation condition for the biocontrol strain of Trichoderma harzianum FJAT-9040 using single factor analysis,Box-Behnken design.and response surface methodology(RSM).The constitute of fermentation condition initial moisture,initial inoculation amount,culture temperature were determined by single factor experiment,then the optimal condition was optimized using Box-Behnken design and RSM.The result of single factor showed that the initial moisture,initial inoculation amount,culture temperature were 50%,5%,28℃,respectively.The results showed that initial moisture and culture temperature were significant for the biomass of strain of T.harzianum based on the response surface methodology analysis(P <0.05).The optimum fermentation conditions were as followed:initial moisture 53%,initial inoculation amount 7%,culture temperature 29℃,the average sporulation yield reached to 4.04×109cfu·g-1,which was closed to the actual 3.86×109 cfu·g-1.
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出版历程
  • 收稿日期:  2014-11-07
  • 刊出日期:  2015-02-17

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