摘要:
为优化生防菌哈茨木霉FJAT-9040固体发酵条件,结合初始含水量、初始接种量及培养温度3个单因素,Box-Behnken设计和响应面分析法进行研究。以产孢量为指标,单因素试验确定初始含水量、初始接种量及培养温度分别为50%、5%、28℃。响应面分析结果表明:初始含水量和温度与产孢量有显著相关性(P<0.05),通过求解回归方程可得,当初始含水量为53%,初始接种量为7%,培养温度29℃时,产孢量达到4.04×109个·g-1,与实际值3.86×109个·g-1相近。
Abstract:
Optimization of solid-state fermentation condition for the biocontrol strain of Trichoderma harzianum FJAT-9040 using single factor analysis,Box-Behnken design.and response surface methodology(RSM).The constitute of fermentation condition initial moisture,initial inoculation amount,culture temperature were determined by single factor experiment,then the optimal condition was optimized using Box-Behnken design and RSM.The result of single factor showed that the initial moisture,initial inoculation amount,culture temperature were 50%,5%,28℃,respectively.The results showed that initial moisture and culture temperature were significant for the biomass of strain of T.harzianum based on the response surface methodology analysis(P <0.05).The optimum fermentation conditions were as followed:initial moisture 53%,initial inoculation amount 7%,culture temperature 29℃,the average sporulation yield reached to 4.04×109cfu·g-1,which was closed to the actual 3.86×109 cfu·g-1.
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