‘农科180’番茄种苗组织培养规模化繁育技术研究

赖呈纯; 黄贤贵; 范丽华; 谢鸿根; 郑回勇; 陈华; 刘波

doi:10.19303/j.issn.1008-0384.2015.02.010

‘农科180’番茄种苗组织培养规模化繁育技术研究

doi: 10.19303/j.issn.1008-0384.2015.02.010

1. 福建省农业科学院农业工程技术研究所;
2. 福建省农业科学院数字农业研究所;
3. 福建省农业科学院

基金项目:

福建省农业科学院设施农业专项(FQ1405)

福建省科技重大专项(2014NZ0002-2)

福建省种业创新与产业化工程项目(2014S1477-1)

详细信息

作者简介:
赖呈纯(1975-),男,博士,副研究员,主要从事园艺植物生物技术与植物细胞代谢工程研究(E-mail:lccisland@163.com);刘波(1957-),男,博士,研究员,主要从事生物资源方面的研究(E-mail:liubofaas@163.com)

中图分类号: S641.2
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出版历程
- 收稿日期: 2014-11-14
- 刊出日期: 2015-02-18

Tissue Culture of ‘Nongke180' Tomato

1. Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences;
2. Institute of Digital Agriculture,Fujian Academy of Agricultural Sciences;
3. Fujian Academy of Agricultural Sciences

Funds:

3. 福建省农业科学院,福建福州,350003

2. 福建省农业科学院数字农业研究所,福建福州,350003

1. 福建省农业科学院农业工程技术研究所,福建福州,350003

摘要

摘要: 以‘农科180’番茄种子为材料,研究其无菌萌发的条件与方法,并以其无菌苗的茎尖、子叶和下胚轴为外植体,研究不同培养基对其无菌株系建立的影响,同时探讨适合其芽苗增殖的培养基。结果表明,种子浸泡10~15h后采用10%(v/v)的次氯酸钠消毒20min,可以获得整齐的无菌苗;不同的外植体诱导产生不定芽所用的培养基不同,其中适合子叶诱导不定芽的培养基为MS+2.0mg·L-1 BA+0.5mg·L-1 IAA,适合茎尖诱导不定芽的培养基为MS+0.1mg·L-1 BA+0.1mg·L-1 IBA;番茄芽苗增殖以MS+0.1mg·L-1BA+0.1mg·L-1 IBA增殖的效果较好。
- 番茄 /
- 组织培养 /
- 外植体 /
- 快速繁殖 /
- 试管苗
Abstract: The condition and method for seed germination of ‘Nongke180'tomato was investigated in this experiment.The effects of different media on establishment for Nongke180'tomato plantlets were studied by using shoot tip,cotyledon and hypocotyl as explant.Meanwhile,the suitable medium for the proliferation of‘Nongke180'tomato fascicular gemma was selected by screening in different media.The results showed that the uniformly-growing aseptic seedlings were obtained by disinfecting Nongke180'tomato seed with 10%(v/v)sodium hypochlorite for 20 minutes after accelerating germination.The different media were used for inducing adventitious buds of different ‘Nongke180'tomato explant.Among the media,the medium MS+2.0 mg·L-1 BA+0.5mg·L-1 IAA was suitable for inducing adventitious buds of its cotyledon,the medium of MS+0.1mg·L-1 BA+0.1mg·L-1 IBA was suitable for inducing adventitious buds of its shoot tip,while MS+0.1mg·L-1 BA+0.1mg·L-1 IBA performed better effect on the proliferation of Nongke180'tomato fascicular gemma.
- tomato(Lycopersicum escnlentum Mill.) /
- tissue culture /
- explant /
- micropropagation /
- plantlet