The Effect of Plant Hormones on Tillering of Chewing Cane Somaclone
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摘要: 以未组织培养的果蔗种苗为对照, 对组培苗后代4种内源激素进行测定分析, 以及通过外源激素处理研究果蔗分蘖机理和调控技术。结果显示:组培苗后代中IAA和CTK含量比对照高, 它们可能在分蘖过程中起作用, ABA和GA3含量与对照无明显差异;外源激素处理显示, 6-BA处理后可以显著提高果蔗的分蘖率, 而NAA对抑制果蔗分蘖有一定的效果。综合内源激素测定和外源激素喷施的结果, 推测影响果蔗组培苗分蘖的因素可能是组培过程中残留的6-BA在起作用。Abstract: With no tissue culture chewing cane seedling was used as control, 4kinds of endogenous hormones of the somaclone offspring were tested, and the mechanism of tillering of chewing cane and control technology was studied by exogenous hormone treatment.Resultsshowed that IAA and CTK content of somaclone offspring were higher than control, which suggested that they might play a role in the process of tillering, but ABA and GA3 content was not significantly different from control.The result of exogenous hormone treatment showed that 6-BA treatment could significantly improve tillering rate of chewing cane, and NAA had the certain effect on the inhibition of chewing cane tillering.Comprehensive the result above, we speculated that the effect factors of chewing cane somaclone tillering might be the 6-BA which was remained in the process of tissue culture.
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Key words:
- chewing cane /
- somaclone /
- endogenous hormones /
- exogenous hormones /
- tillering
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[1] IRVINE JE, BENDA GTA.Sugarcane mosaic virus in plantlets regenerated from detached leaf tissue[J].Plant Cell, Tissue and Organ Culture, 1985, 5 (2) :101-106. [2] 贤武, 王伦旺, 王天算, 等.甘蔗茎尖脱毒培养研究初报[J].广西蔗糖, 2000, (4) :1-13. [3] 许莉萍, 陈如凯, 李跃平.利用愈伤组织培养和茎尖培养去除甘蔗花叶病毒[J].福建农业大学学报, 1994, 23 (3) :253-256. [4] LAUDE H M.Tiller senescence and grain development in barley[J].Crop Science, 1976, 7:261-263. [5] MICHAEL G, BERINGER H.The role of hormones in yield formation, physiological aspects of group productivity//Proceeding of the Colloquium of the International Potash Institute held in Wageninien[J].The NetherLands, 1980, 28:252-279. [6] 刘杨, 丁艳锋, 王强盛, 等.植物生长调节剂对水稻分蘖芽生长和内源激素变化的调控效应[J].作物学报, 2011, 37 (4) :670-676. [7] 敬成俊, 李昌庭, 邓亚平, 等.秋水仙素间断式诱导植物多倍体研究[J].西南大学学报:自然科学版, 2013, 35 (7) :45-51. [8] 李春喜, 赵广才, 代西梅, 等.小麦分蘖变化动态与内源激素关系的研究[J].作物学报, 2000, 26 (6) :963-968. [9] 王纪华, 王树安, 蒋钟怀.小麦子粒建成过程的光合特性及内源激素的研究[J].北京农业大学学报, 1992, 18 (S1) :167-172. [10] 张军, 韩碧文, 何钟佩, 等.植物激素酶联免疫测定基础[J].北京农业大学学报, 1991, 17 (S1) :139-148. [11] 智利婷, 洪培培, 陈洪伟, 等.外源植物激素对一串红侧芽生长的影响[J].北方园艺, 2013, (7) :52-59.
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