Effects of Colchicine on Mutagenesis of OncidiumIn Vitro
-
摘要: 以秋水仙素为诱变剂, 结合组织培养技术, 对文心兰丛生芽和原球茎进行诱变试验, 并应用分子标记技术检测诱变后代的变异情况。结果表明:培养基添加秋水仙素混合培养法是文心兰丛生芽诱变的最佳途径, 以添加500mg·L-1的秋水仙素诱导10d效果最佳, 植株变异率高达26.7%, 变异植株类型包括植株粗壮、多叶、叶片变宽、变厚、矮化、叶片线艺等;经56次继代培养, 部分变异株系仍保持稳定遗传性状, 其中筛选出的稳定绿叶金边、金叶绿边变异株系RAPD标记表明其在DNA水平上发生了变异, 为文心兰育种提供了新的方法和思路。Abstract: With colchicine was used as the mutagenic agent, combined with tissue culture techniques, the mutagenesis experiments for bud clump and PLB of Oncidium and the molecular marker technology to detect mutagenesis progeny were studied.Resultsshowed that method of mixed medium supplemented with colchicine was the best way to mutage the Oncidium, and the best condition was 10 dinduction with 500 mg·L-1of colchicine.Plant mutation rate was as high as 26.7%, and there were different mutation forms, such as hairchested, polyphyllous, dwarfed plantlets and widened, thickened leaves, and linear, patterned leaves.Mutant strain went through 56successive transfer culture, and part of them remained properties well.Among them, the variation with golden margin leaves and variation with green margin golden leaves had apparent variation compared with the original species on DNA level by RAPD markers.It suggested a new breeding methods and ideas for Oncidium.
-
Key words:
- Oncidium /
- culture in vitro /
- protocorm-like body /
- colchicines /
- chemical mutagenesis
-
[1] 何松林, 十鸟三和子, 王献, 等.不同基本培养基及培养方式对文心兰原球茎增殖的影响[J].华北农学报, 2001, 16 (1) :88-91. [2] 何松林, 十鸟三和子.基本培养基及凝固剂对文心兰试管苗生长发育的影响[J].北京林业大学学报, 2001, 23 (1) :29-31. [3] 丁兰, 付庭治.兰花生物工程研究进展[J].西北师范大学学报, 2000, 36 (3) :111-115. [4] 叶炜, 尚伟, 夏潮水.文心兰育种研究进展[J].三明农业科技, 2008, (3) :17-19. [5] 邱金.文心兰的杂交育种[J].花卉, 2010, (4) :18-19. [6] 范成明, 李枝林, 何月秋.兰花组织培养及分子生物学研究进展[J].园艺学报, 2003, 30 (4) :487-491. [7] 刘玉进, 郑成木.诱变结合植物组织培养在植物育种中的应用[J].上海农业学报, 2004, (1) :19-22. [8] 崔广荣, 上官凌飞, 张子学, 等.文心兰类原球茎液体增殖过程中秋水仙素化学诱变[J].园艺学报, 2009, 16 (9) :1385-1389. [9] 崔广荣, 张子学, 张从宇, 等.文心兰多倍体诱导及其鉴定[J].草业学报, 2010, 19 (1) :184-190. [10] 叶秀仙, 黄敏玲, 吴建设等.文心兰茎尖诱导丛生芽高频率植株再生[J].福建农业学报, 2009, 24 (2) :126-137. [11] 叶秀仙, 黄敏玲, 钟淮钦, 等.文心兰离体再生体系建立及试管苗种质保存研究.中国观赏园艺研究进展2009[M].北京:中国林业出版社, 2009:193-197. [12] 肖军, 杨立国, 杨涛, 等.两种提取菊花总DNA的方法比较[J].辽宁农业科学, 2005, (1) :40-41. [13] 刘进平, 郑成木.诱变结合植物组织培养在植物育种中的应用[J].上海农业学报, 2004, 20 (1) :19-22. [14] 董颖苹, 连勇, 何庆才, 等.植物化学诱变技术在育种中的运用及其进展[J].种子, 2005, 24 (7) :54-58. [15] 瞿素萍, 熊丽, 莫锡君, 等.香石竹的多倍体诱导及其变异研究[J].西南农业大学学报, 2004, (5) :609-612. [16] 何林, 张洁, 郭启高, 等.东方百合Tiber多倍体诱导及其快繁研究[J].西南农业大学学报, 2006, (6) :945-949. [17] 敬成俊, 李昌庭, 邓亚平, 等.秋水仙素间断式诱导植物多倍体研究[J].西南大学学报:自然科学版, 2013, 35 (7) :45-51. [18] 张志胜, 谢利, 萧爱兴, 等.秋水仙素处理兰花原球茎对其生长和诱变效应的影响[J].核农学报, 2005, 19 (1) :19-23. [19] 敬成俊, 李昌庭, 邓亚平, 等.秋水仙素间断式诱导植物多倍体研究[J].西南大学学报:自然科学版, 2013, 25 (7) :45-51. [20] FELIX L P, GUERRA M.Basic chromosome numbers of terrestrial orchids[J].Plant Systematics and Evolution, 2005, 254 (3) :131-148. -
点击查看大图
计量
- 文章访问数: 151
- HTML全文浏览量: 33
- PDF下载量: 0
- 被引次数: 0
下载:
