洗涤厂排污口可培养中度嗜热菌的多样性

叶学军; 邱宏端; 林新坚; 贾宪波; 陈济琛

doi:10.19303/j.issn.1008-0384.2014.08.011

洗涤厂排污口可培养中度嗜热菌的多样性

doi: 10.19303/j.issn.1008-0384.2014.08.011

1. 福州大学生物科学与工程学院，福建福州 350108;
2. 福建省农业科学院土壤肥料研究所，福建福州 350013

基金项目:

福建省星火计划项目 (2012S0061)

国家公益性行业 (农业) 科研专项 (201303094-05)

福建省财政厅社会公益研究项目 (2060302)

福建省科技计划项目——省属公益类科研院所基本科研专项 (2009R10002-2)

详细信息

作者简介:
叶学军 (1987-), 男, 在读硕士研究生, 研究方向:应用微生物学 (E-mail:yexuejun01@163.com);邱宏端 (1955-), 女, 教授, 研究方向:应用微生物学 (E-mail:hongduanlq@163.com);陈济琛 (1964-), 男, 研究员, 研究方向:农业微生物和食用菌 (E-mail:chenjichen2001@163.com)

中图分类号: Q938.1
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出版历程
- 收稿日期: 2014-03-31
- 刊出日期: 2014-08-18

Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory

1. College of Biological Science and Engineering,Fuzhou University;
2. Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences

摘要

摘要: 为考查洗涤厂热废水排放口土壤在常年人为热环境下的可培养嗜热菌的多样性, 采集洗涤厂热废水排放口附近的湿热泥土, 应用不同培养基分离纯化泥土中的可培养嗜热菌, 并对获得的纯培养细菌进行16SrDNA鉴定和系统发育相关分析。结果表明:分离纯化得到的29株不同表型的纯培养菌株均属于坚壁菌门芽孢杆菌纲, 归属于5个属11个种, 分别是地芽孢杆菌属Geobacillus 3个种, 芽孢杆菌属Bacillus 3个种, 无氧芽孢杆菌属Anoxybacillus 3个种, 解脲芽孢杆菌属Ureibacillus 1个种和高温放线菌属Thermoactinomyces 1个种, 属种多样性丰富, 菌株资源具有潜在开发应用价值。
- 洗涤厂 /
- 可培养中度嗜热菌 /
- 分离 /
- 鉴定 /
- 多样性
Abstract: To investigate the diversity of mesophilic bacteria resulting from constant thermal conditions, the soil sample was collected at outfall of thermal wastewater from laundry factory.The culturable mesophilic bacteria were isolated with different medium to obtain pure culture, identified by 16 SrDNA and the data were analyzed in phylogenetic tree.The results showed that the 29 strains in pure culture were obtained from the thermal wastewater outfall soil with different phenotype.The strains were classified into Bacilli, attributed to 5genus and 11 species, including three species of Geobacillus, three species of Bacillus, three species of Anoxybacillus, one species of Ureibacillus, and one species of Thermoactinomyces.The diversity of species was rich, and the resources of species would have potential in practical application.
- laundry factory /
- culturable mesophilic bacteria /
- isolation /
- identification /
- diversity

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