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鹤望兰二氢黄酮醇-4-还原酶基因SrDFR的克隆及表达分析

樊荣辉 黄敏玲 钟淮钦 吴建设

樊荣辉, 黄敏玲, 钟淮钦, 吴建设. 鹤望兰二氢黄酮醇-4-还原酶基因SrDFR的克隆及表达分析[J]. 福建农业学报, 2014, 29(8): 748-751. doi: 10.19303/j.issn.1008-0384.2014.08.008
引用本文: 樊荣辉, 黄敏玲, 钟淮钦, 吴建设. 鹤望兰二氢黄酮醇-4-还原酶基因SrDFR的克隆及表达分析[J]. 福建农业学报, 2014, 29(8): 748-751. doi: 10.19303/j.issn.1008-0384.2014.08.008
FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she. Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae[J]. Fujian Journal of Agricultural Sciences, 2014, 29(8): 748-751. doi: 10.19303/j.issn.1008-0384.2014.08.008
Citation: FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she. Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae[J]. Fujian Journal of Agricultural Sciences, 2014, 29(8): 748-751. doi: 10.19303/j.issn.1008-0384.2014.08.008

鹤望兰二氢黄酮醇-4-还原酶基因SrDFR的克隆及表达分析

doi: 10.19303/j.issn.1008-0384.2014.08.008
基金项目: 

福建省科技重大专项 (2010NZ0003、2012NZ0002)

福建省财政专项——福建省农业科学院科技创新团队建设项目 (CXTD-2-17、CXTD-2-1317)

详细信息
    作者简介:

    樊荣辉 (1983-), 女, 助理研究员, 研究方向:花卉分子育种;黄敏玲 (1960-), 女, 研究员, 研究方向;花卉分子育种 (E-mail:huangml618@163.com)

  • 中图分类号: S682.19

Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae

  • 摘要: 采用RT-PCR方法从鹤望兰蓝色花瓣中克隆到类黄酮合成途径关键基因SrDFR, 该片段长246bp, 编码82个氨基酸。氨基酸同源性分析表明, SrDFR推导的氨基酸序列与已报道的其他植物的DFR蛋白具有很高的同源性。系统进化树分析显示, 鹤望兰SrDFR与姜荷花聚为一类, 亲缘关系较近。应用半定量PCR分析表明, SrDFR在蓝色花瓣中高表达, 在黄色花萼和叶片中低表达, 且在花发育的始花期表达量最高, 表明该基因在蓝色花瓣发育过程中起着重要作用。
  • [1] MOL J, COMISH E, MASON J, et al.Novel coloured flowers[J].Curr Opin Biotechnol, 1999, (10) :198-201.
    [2] 黄金霞, 王亮生, 李晓梅, 等.花色变异的分子基础与进化模式研究进展[J].植物学通报, 2006, 23 (4) :321-333.
    [3] KRISTIANSEN K N, ROHDE W.Structure of the Hordeum vulgare gene encoding dihydroflavonol-4-reductase and molecular analysis of ant18 mutants blocked in flavonoid synthesis[J].Mol Gen Genet, 1991, 230 (1-2) :49-59.
    [4] MARTENS S, TEERI T, FORKMANN G.Heterologous expression of dihydroflavonol 4-reductases from various plants[J].FEBS Lett, 2002, 531 (3) :453-458.
    [5] PETERS D J, CONSTABEL C P.Molecular analysis of herbivore-induced condensed tannin synthesis:cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides) [J].Plant J, 2002, 32 (5) :701-712.
    [6] NAKATSUKA A, IZUMI Y, YAMAGISHI M.Spatial and temporal expression of chalcone synthase and dihydroflavonol 4-reductase genes in the Asiatic hybrid lily[J].Plant Science, 2003, 165:759-767.
    [7] MEYER P, HEIDMANN I, FORKMANN G, et al.A new petunia flower colour generated by transformation of amutant with a maize gene[J].Nature, 1987, 330:677-678.
    [8] AIDA R, SANAE K, YOSHIKAZU T, et al.Modification of flow er color in torenia by genetic transformation[J].Plant Sci, 2000, 153:32-42.
    [9] AIDA R, YOSHIDA K, KONDO T, et al.Copigmentation gives bluer flowers on transgenic torenia plants with the antisense dihydroflavonol-4-reductase gene[J].Plant Sci, 2000, 160:49-56.
    [10] BONGUE-BARTELSMAN M, O′NEILL S D, TONG Y, et al.Characterization of the gene encoding dihydroflavonol 4-reductase in tomato[J].Gene, 1994, 138 (1-2) :153-157.
    [11] HELARIUTTA Y, ELOMMAI P, KOTILAINEN M, et al.Cloning of cDNA coding for dihydroflavonol 4-reductase (DFR) and characterization of dfr expression in the corollas of gerbera hybrida var.Regina (Compositeae) [J].Plant Mol Biol, 1993, 22 (2) :183-193.
    [12] XIE D Y, JACKSON L A, COOPER J D, et al.Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from medicagotruncatula[J].Plant Physiol, 2004, 134 (3) :979-994.
    [13] SPARVOLI F, MARTIN C, SCIENZA A, et al.Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.) [J].Plant Mol Biol, 1994, 24 (5) :743-755.
    [14] BELD M, MARTIN C, HUITS H, et al.Flavonoid synthesis in Petunia hybrida:partial characterization of dihydroflavonol-4-reductase genes[J].Plant Mol Biol, 1989, 13 (5) :491-502.
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出版历程
  • 收稿日期:  2014-05-12
  • 刊出日期:  2014-08-18

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