禽坦布苏病毒包膜蛋白E的基因特征

万春和; 施少华; 黄瑜

doi:10.19303/j.issn.1008-0384.2014.08.001

禽坦布苏病毒包膜蛋白E的基因特征

doi: 10.19303/j.issn.1008-0384.2014.08.001

福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心,福建福州,350013

基金项目:

现代农业产业技术体系建设专项 (CARS-43)

福建省自然科学基金项目 (2012J01112)

详细信息

作者简介:
万春和 (1982-), 男, 博士在读, 助理研究员, 主要从事水禽病毒分子生物学研究;黄瑜 (1965-), 男, 博士, 研究员, 主要从事动物传染病研究 (E-mail:huangyu_815@163.com)

中图分类号: S852.65
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出版历程
- 收稿日期: 2014-04-02
- 刊出日期: 2014-08-18

Genetic Feature of the Envelop Potein of Avian Tembusu Virus

Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Science/Fujian Animal Disease Control Technology Development Center

摘要

摘要: 为明确禽坦布苏病毒包膜蛋白E的基因特征, 运用RT-PCR从已经分离鉴定的禽坦布苏病毒中扩增出其包膜蛋白E全基因, 并进行克隆测序和分析。结果表明, 禽坦布苏病毒包膜蛋白E全长为1 503bp, 编码501个氨基酸。所编码的包膜蛋白E大小为54.217ku, 理论等电点为6.89, 不稳定系数为25.48, 属于稳定蛋白质类。利用TMHMM2.0进行跨膜区分析发现, 该蛋白的拓扑结构为o442-464i471-489o。本试验株包膜蛋白E和北京鸭源坦布苏病毒 (GenBank号:JF459991) 同源性最高, 达99.8%, 和雌麻鸭源坦布苏病毒 (GenBank号:JQ928189) 核苷酸同源性最低, 为98.2%, 不同来源 (鸡源、鸭源、鹅源、鸽源和蚊子源) 坦布苏病毒包膜蛋白E同源性均较高, 没有表现出宿主特异性。
- 禽坦布苏病毒 /
- 包膜蛋白 /
- 基因特征
Abstract: The envelop protein fragments of avian Tembusu virus were amplified by RT-PCR from the identified avian Tembusu virus isolated in Fujian, and cloned to the T-vector for sequenceing and bioinformatics analysis.The results revealed that the avian Tembusu virus envelop gene contained 1 053 nucleotides, coding an open reading frame (ORF) with 501 amino acids.The molecular weights, theoretical isoelectric point and the instability index of avian Tembusu virus envelop protein were 54.217 ku, 6.89 and 25.48, respectively.The topology predicted by Nbest using the TMHMM2.0is o442-464i471-489 o.The sequenced envelop protein shared the highest homogeneity with Peking duck-origin avian Tembusu virus strain (GenBank accession number is JF459991) , which was 99.8%, while shared with common sheldduck-origin avian Tembusu virus strain (GenBank accession number is JQ928189) 98.2% homogeneity.The results demonstrated that avian Tembusu virus had no host specificity
- avian Tembusu virus /
- envelop protein /
- molecular analysis

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