睾丸酮降解菌的分离鉴定及降解特性

白泽方; 潘大仁; 霍煜璟; 李清

doi:10.19303/j.issn.1008-0384.2014.07.019

睾丸酮降解菌的分离鉴定及降解特性

doi: 10.19303/j.issn.1008-0384.2014.07.019

1. 福建农林大学作物科学学院,福建福州,350108;
2. 福建农林大学生命科学学院,福建福州,350108

基金项目:

福建省自然科学基金项目 (2008J0041)

详细信息

作者简介:
白泽方 (1987-), 男, 在读硕士生, 研究方向:农业生物技术 (E-mail:xiaobai2006jay@126.com);潘大仁 (1949-), 男, 博士, 教授, 研究方向:农业生物技术 (E-mail:pdr8598@163.com)

中图分类号: X172
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- 文章访问数: 154
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- 被引次数: 0
出版历程
- 收稿日期: 2014-05-03
- 刊出日期: 2014-07-18

Isolation and Characterisation of a Testosterone-Degrading Bacterium

1. College of Crop Science,Fujian Agriculture and Forestry University;
2. College of Life Sciences,Fujian Agriculture and Forestry University

摘要

摘要: 为筛选获得具有降解环境雄激素的菌株, 从福建莆田的土壤中分离得到1株能够高效降解睾丸酮的菌株, 命名为C53-20。该菌经过常规的形态和16SrDNA序列同源性分析, 鉴定为克雷伯氏肺炎菌 (Klebsiella pneumonia) 。该菌在35⁴0℃、pH6.0⁸.0具有较高的降解活性, 其最适值分别为温度37℃和pH6.5。该菌能够以睾丸酮为唯一碳源生长, 12h内对1mmol·L-1睾丸酮的降解率为45%。该菌的筛选获得为进一步研究与睾丸酮降解相关的微生物基因提供资源。
- 降解菌 /
- 睾丸酮 /
- 16SrDNA
Abstract: One bacterium, which has testosterone degradation efficiently, was isolated from the sludge, Fujian and named C53-20.The results of morphological characteristics and the similarity analysis of the 16 SrDNA sequence suggest that it is a Klebsiella pneumonia.In addition, testosterone degradation activity in 35-40℃and pH6.0-8.0range was assayed, and our results suggest that the optimum activity is at 37℃and pH6.5, respectively.Testosterone can be utilized as sole carbon and energy sources and the degradation rate to cholesterol was 45%, with1mmol·L-1 concentrations within 12 hours.Our results suggest that the strain has potential application prospects in environmental remediation of androgen contaminated areas.
- degrading bacteria /
- testosterone /
- 16S rDNA

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