A New Zoophthora Occidentalis Pathogen of Myzus Persicae in China
-
摘要: 从福建省邵武市的桃蚜上分离出1株病原真菌, 通过形态学鉴定为西虫瘟霉Zoophthora occidentalis。其初生分生孢子长椭圆形, 有明显的孢领, 大小为 (23.431.2) μm× (7.810.9) μm, L/D=2.43.8。次生分生孢子与初生分生孢子同形, 大小为 (15.625.4) μm× (7.810.4) μm, L/D=2.464.08。毛管孢子大小为 (20.829.9) μm× (6.010.4) μm, L/D=2.43.6, 柄长36.4104.0μm。菌丝直径6.58.8μm。休眠孢子表面光滑, 无色透明, 直径23.929.9μm。用“孢子浴”方法将该菌对桃蚜接种, 结果显示接种后第4d为死亡高峰期, 最终累计死亡率5.4%95.7%, 因剂量而异。经时间-剂量-死亡率模型分析, 测得接种4d后LD50低于10个孢子·mm-2, 表明该菌株对桃蚜具有较强的毒力。Abstract: A newly discovered fungal pathogen isolated from the green peach aphids, Myzus persicae, in Fujian was identified as Zoophthora occidentalis.Its elongate ovoid primary conidia sized (23.4-31.2) μm× (7.8-10.9) μm (L/D =2.4-3.8) and had a clear spore collar.Secondary conidia liked primary (15.6-25.4) μm× (7.8-10.4) μm (L/D =2.46-4.08) was also oblong in shape (15.6-25.4) μm× (7.8-10.4) μm with an L/D ratio of 2.46-4.08.The size of its capilliconidium was (20.8-29.9) μm× (6.0-10.4) μm (L/D =2.4-3.6) , and the stipe was 36.4-104.0μm long.The diameter of its mycelium was 6.5-8.8μm.Its resting spores were smooth, colorless and transparent with a diameter ranging from 23.9μm to 29.9μm.Most of the infected aphids were killed in the 4th day, and green peach aphids to induce a peaked death rate in 4days and cumulative mortality ranging from 5.4%to 95.7%, which increased with the spore concentration.The resulting data fitted well on a time-dose-mortality model.The LD50 after 4days estimated by the model was less than 10conidias·mm-2, indicating the entomophthoralean fungus to be a potent pathogen for M.persicae.
-
Key words:
- Zoophthora occidentalis /
- Myzus persicae /
- bioassay /
- entomophthorales
-
[1] BARTA M, CAGNL′.Aphid-pathogenic entomophthorales (their taxonomy, biology and ecology) [J].Biologia, 2006, 61 (21) :543-616. [2] AVERY M.Record of a Zoophthora sp. (Entomophthoromycota:Entomophthorales) pathogen of the irruptive noctuid moth Eurois occulta (Lepidoptera) in West Greenland[J].Journal of Invertebrate Pathology, 2013, 114 (3) :292-294. [3] BALAZY S.Flora of Poland, Fungi (Mycota) Vol.24, Entomophthorales[M].Polish Academy of Science, 1993:1-356. [4] 李增智.中国真菌志虫霉目[M].北京:科学出版社, 2000:1-168. [5] 张广学, 钟铁森.中国经济昆虫志, 第二十五册, 同翅目:蚜虫类 (一) [M].北京:科学出版社, 1983:1-387. [6] 黄耀坚, 郑本暖.福建虫霉的种类、分布、流行及应用策略[J].福建林学院学报, 1990, 10 (1) :49-56. [7] KELLER S, PETRINI O.Keys to the identification of the arthropod pathogenic genera of the families Entomophthoraceae and Neozygitaceae (Zygomycetes) , with descriptions of three new subfamilies and a new genus[J].Sydowia, 2005, 57 (1) :23-53. [8] MILNER R J, TEAKLE R E, LUTTON G G, et al.Pathogens (Phycomycetes:Entomophthoraceae) of the bluegreen aphid Acyrthosiphon kondoi Shinji and other aphids in Australia[J].Australian Journal of Botany, 1980, 28:601-619. [9] KELLER S.Fungal structures and biology[C]//KELLER S Arthropod-Pathogenic Entomophthorales:Biology, Ecology, Identification.Luxembourg:Office for Official Publications of the European Communities, 2007:66-77. [10] 刘彩玲, 冯明光.安徽虫瘟霉对桃蚜的生物测定与时间-剂量效应分析[J].菌物系统, 1998, 17 (4) :361-366. [11] 许谦, 冯明光.生物测定根虫瘟霉对桃蚜的毒力[J].浙江农业大学学报, 1999, 25 (2) :151-154. [12] FENG M G, JOHNSON J B.Bioassay of four Entomophthorales fungi (Entomophthorales) against Diuraphis noxia and Metopolophium dirhodum (Homoptera:Aphididae) [J].Environmental Entomology, 1991, 20:1699-1710. [13] 唐启义, 冯明光.DPS数据处理系统[M].北京:中国农业出版社, 1997:1-648. [14] KELLER S.Entomophthorales attacking aphids with a description of two new species[J].Sydowia, 2006, 58:38-74. -
点击查看大图
计量
- 文章访问数: 173
- HTML全文浏览量: 46
- PDF下载量: 1
- 被引次数: 0
下载:
