Effects on Immune Organ Index and Humoral Immunity in Duck Infected With Duck Tembusu Virus
-
摘要: 鸭坦布苏病毒是近年来在我国发现的可引起种 (蛋) 鸭高热、采食量和产蛋量骤降的新病毒。本研究以麻鸭为动物模型, 测定麻鸭感染鸭坦布苏病毒后, 其免疫器官指数、重组H5亚型禽流感病毒灭活疫苗和鸡新城疫病毒弱毒活疫苗诱导产生的抗体水平。结果表明, 鸭坦布苏病毒感染会侵害麻鸭的免疫器官, 第13d脾脏明显肿大, 第710d试验组胸腺指数极显著低于对照组, 第114d法氏囊出现萎缩;与仅免疫疫苗的对照组鸭相比, 感染鸭坦布苏病毒后再注射疫苗的试验组鸭产生抗体的时间延迟、抗体的峰值降低, 抗体下降加快, 且在整个试验期内产生的抗体水平均低于对照组鸭的抗体水平, 表明鸭坦布苏病毒感染可抑制鸭对灭活疫苗和弱毒疫苗的体液免疫应答能力。Abstract: Duck Tembusu virus is a newly discovered virus in recent years, which caused duck high fever, egg-laying and feed intake decreased.In this study, we used Sheldrake as animal model, and infected with duck Tembusu virus.Then we determinated the immune organ indices and the antibody levels in ducks induced by recombinant H5 avian influenza inactivated vaccine and Newcastle disease virus attenuated live vaccine, respectively.The results showed that the duck immune organs were violated after infected with duck Tembusu virus, resulting in spleen enlargement (1dpi to 3dpi) , bursa atrophy (1dpi to 14dpi) , and the test group thymus index was significantly lower than the control group from 7dpi to 10 dpi.Compared with immunized-group, the antibodies level of the infected-immunized-group were delayed and low, and reduction in peaks and declined accelerate throughout the test period.The above results indicated that duck Tembusu virus can affect the humor immune response to inactivated vaccine and attenuated vaccine in duck.
-
Key words:
- duck Tembusu virus /
- immune organ index /
- antibody /
- humoral immunity
-
[1] 曹贞贞, 张存, 黄瑜, 等.鸭出血性卵巢炎的初步研究[J].中国兽医杂志, 2010, 46 (12) :3-6. [2] 万春和, 施少华, 程龙飞, 等.一种引起种 (蛋) 鸭产蛋骤降新病毒的分离与初步鉴定[J].福建农业学报, 2010, 25 (6) :663-666. [3] SU J, LI S, HU X, et al.Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus[J].PLoS One, 2011, 6 (3) :e18106. [4] 万春和, 黄瑜.坦布苏病毒致病特性及其流行病学[J].动物医学进展, 2011, 32 (12) :116-118. [5] 刘友生, 彭春香, 黄瑜, 等.2010-2011年中国部分地区禽坦布苏病毒感染调查及分子变异分析[J].中国动物传染病学报, 2012, 20 (1) :47-53. [6] 施少华, 傅光华, 万春和, 等.鹅源坦布苏病毒的分离及初步鉴定[J].中国兽医杂志, 2012, (12) :37-40. [7] 袁生, 李金平, 张浩吉, 等.鹅源黄病毒的分离和初步鉴定[J].中国畜牧兽医, 2012, 39 (9) :47-49. [8] 傅光华, 黄瑜, 施少华, 等.鸡黄病毒的分离与初步鉴定[J].福建畜牧兽医, 2011, 33 (3) :1. [9] 赵圆圆, 李传峰, 王桂军, 等.鸭产蛋下降-死亡综合征的临床诊断与病原的初步鉴定[J].扬州大学学报:农业与生命科学版, 2011, 32 (3) :11-14. [10] 张大丙.鸭出血性卵巢炎的研究进展[J].中国家禽, 2011, 33 (14) :37-38. [11] 刘志刚, 孙青松, 姚蓉, 等.鸭坦布苏病毒研究进展[J].中国动物传染病学报, 2013, 21 (1) :81-86. [12] 郭鑫.动物免疫学实验教程[M], 北京:中国农业大学出版社, 2007:30-50. [13] 崔治中, 崔保安.兽医免疫学[M], 北京:中国农业出版社, 2009:13-19. [14] 陈雪锋.REV感染SPF鸡中IL-6、IL-18和IFNγ的定量检测[D].泰安:山东农业大学, 2012. [15] 朱海侠.鸭出血症弱毒活疫苗免疫功能研究[D].福州:福建农林大学, 2012. [16] 王建新.J亚群禽白血病病毒感染对肉鸡生长和免疫功能的影响及其env基因的克隆[D].泰安:山东农业大学, 2003. [17] 李爽, 谷长勤, 白家媛, 等.鸭源呼肠孤病毒感染引起雏鸭免疫损伤的观察[J].中国农业科学, 2010, 43 (21) :4514-4520. [18] 商营利.鸡混合感染J亚群白血病病毒与网状内皮增殖病病毒免疫抑制机理研究[D].泰安:山东农业大学, 2003, 25-30. [19] 刘应鹏, 刘兴友, 王艳林, 等.鸡病毒性免疫抑制病的研究进展[J].中国畜牧兽医.2005, 32 (5) :57-60. [20] 曹宗喜, 潘全会, 黄伟胜, 等.鸡免疫抑制性疾病的免疫抑制机理研究[J].中国家禽.2008, 30 (18) :34-37. [21] 王学理, 李向阳, 龚团莲.鸡传染性贫血病研究进展[J].甘肃畜牧兽医, 2005, 4 (183) :40-44. [22] SOIKE D, ALBRECHT K, HATTERMANN K, et al.Novel circovirus in Mulard ducks with developmental and feathering disorders[J], Vet Rec, 2004, 154:792-793. -
点击查看大图
计量
- 文章访问数: 200
- HTML全文浏览量: 44
- PDF下载量: 3
- 被引次数: 0
下载:
