莲雾黑腐病病原菌的鉴定及其生物学特性研究

车建美; 刘波; 阮传清; 王国芬; 黄丹丹; 杨忠来; 郭慧慧

doi:10.19303/j.issn.1008-0384.2014.06.014

莲雾黑腐病病原菌的鉴定及其生物学特性研究

doi: 10.19303/j.issn.1008-0384.2014.06.014

1. 福建省农业科学院农业生物资源研究所,福建福州,350003;
2. 中国热带农业科学院环境与植物保护研究所,海南海口,571737;
3. 福建农林大学生命科学学院,福建福州,350002

基金项目:

中国热带农业科学院中央级公益性科研院所基本科研专项 (1630042012006)

福建省科技计划区域重大项目 (2011N3007)

农业科技成果转化项目 (2012GB2C400218)

国家自然科学基金项目 (31370059)

详细信息

作者简介:
车建美 (1977-), 女, 副研究员, 博士, 研究方向:微生物生物技术、微生物保鲜 (E-mail:chejm2002@163.com)刘波 (1957-), 男, 博士, 研究员, 研究方向:微生物生物技术及农业生物药物 (E-mail:fzliubo@163.corn);车建美 (1977-), 女, 副研究员, 博士, 研究方向:微生物生物技术、微生物保鲜 (E-mail:chejm2002@163.com)刘波 (1957-), 男, 博士, 研究员, 研究方向:微生物生物技术及农业生物药物 (E-mail:fzliubo@163.corn)

中图分类号: S436.67
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- 文章访问数: 217
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- PDF下载量: 3
- 被引次数: 0
出版历程
- 收稿日期: 2013-09-28
- 刊出日期: 2014-06-18

Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics

1. Agricultural Bio-Resource Research Institute,Fujian Academy of Agricultural Sciences;
2. Environment and Plant Protection Research Institute,CATAS;
3. College of Life Science,Fujian Agriculture and Forestry University

摘要

摘要: 通过病害症状观察、分离菌致病性测定, 以及分离菌株的形态和培养性状观察、rDNA-ITS序列分析将从莲雾果实上分离到的菌株FJAT-9860鉴定为可可毛色二孢菌Lasiodiplodia theobromae。菌株FJAT-9860在PDA培养基上菌落最初为白色, 后逐渐变为灰色到黑色。分生孢子为单细胞, 无色透明, 椭圆形至圆形。ITS序列分析表明, 菌株FJAT-9860与Lasiodiplodia theobromae strain亲缘关系最近, 同源性为99%。该菌株最适生长温度为25℃, 最适生长pH值为8.0, 不同碳源对菌株FJAT-9860的生长影响不同, 最佳碳源为甘露醇。
- 莲雾 /
- 黑腐病 /
- 鉴定 /
- 生物学特性
Abstract: In order to identify the causal agent of black spot disease on wax apple (Syzygium samarangense Merr.et Perry) in its taxonomic status, the causal fungi FJAT-9860 was isolated and characterized with pathogenicity test, morphology and culture feature observation, rDNA-ITS sequences assay and biological characteristics test.The results indicated that the causal agent belong to Lasiodiplodia theobromae.The colony of strain FJAT-9860 on PDA was white at first, then gradually became as grey to black.The monadic conidia were elliptic to round, and colourless.The rDNA-ITS sequence of the strain shared 99%identity with that of Lasiodiplodia theobromae strain.The results showed that the optimum temperature for mycelium growth of this pathogen was 25℃and the optimum pH was 8.0.The mycelial growth was affected by different carbon sources and the best one was mannitol.
- wax apple (Syzygium samarangense Merr.et Perry) /
- black rot /
- identification /
- biological characteristics

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参考文献(1)

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