摘要:
采用生态化学计量与形态计量方法, 评价紫云英种质不同品种的种子计量学特征, 以期作为紫云英品种 (系) 鉴别分类指标。11个紫云英品种的千粒重马溪种最大, 平均达3.40g, 光泽种和闽紫3号最小, 分别平均为3.05g和3.11g, 表征了紫云英马溪种的特性, 但不能作为早熟品种的特征;11个品种种子表面积闽紫5号最小为3.46mm2, 马溪种面积最大, 均值4.12mm2;其种子形态, 肾形和近肾型种子所占比例范围为83%96.7%, 闽紫7号所占的肾形比率最高为96.7%, 闽紫4号和闽紫5号种子为最低, 只占83%, 差异达到了显著水平。从生态化学计量看, 早熟品种C/N和C/P比值居高, 如光泽种、闽紫3号。通过聚类分析与主成分分析, 闽紫2号、闽紫5号在种质特性方面明显区别于其余9个品种。总之, 紫云英种子的千粒重、面积与外观形状特征不易区别不同基因型的品种, 但可作为个体区别于其他品种的参考指标。种子C/N和C/P比值可以作为紫云英早、晚熟的参考指标之一。
Abstract:
The seed metrology characteristics in different Milk vetch (MV) germplasm were evaluated with morphology and ecological stoichiometry methods and were used in classification for MV germplasm.Comparing the thousand grain weight among 11 Milk vetch varieties, the heaviest one was found in‘Maxi'with average 3.40 g and the lighter were ‘Guangze'and ‘Minzi 3'with 3.05 g and 3.11 g, respectively.The thousand grain weight indicated the‘Maxi'variety in yield but not for its early ripe character.The total surface area of seed among 11 varieties, the smallest one was‘Minzi 5'with 3.46mm2 and the biggest was‘Maxi'with 4.12mm2.The seed form was reniform and subreniform with the proportion for 83% 96.7% of total seeds.The highest ratio of reniform seed was found in‘Minzi 7'with 96.7%, while it in‘Minzi 4'and‘Minzi 5'was similar as the lowest with 83%.The difference between the highest and the lowest ratios was significant.With correlation analysis, the data showed that the surface area of seed was correlated positively with thousand grain weight (r=0.698*) .The ratios in C/N and C/P were higher for early ripe varieties, such as‘Guangze', than for other varieties.Although the indexes in the thousand grain weight, surface area and morphology of MV seed could not be used in differentiation for the genotype of the varieties, the indexes might be referred in use for identifying of the varieties.Ratios in C/N and C/P could be used in identification for Milk vetch character in early or late ripe varieties.
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