A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe
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摘要: 为解决细叶石仙桃人工栽培种苗短缺问题, 以细叶石仙桃蒴果为材料, 应用植物组织培养技术, 筛选不同阶段所需的最适培养基。结果表明其种子可在MS+20g·L-1蔗糖+8g·L-1琼脂培养基上萌发, 发芽率可达70%以上;MS+50g·L-1土豆+20g·L-1蔗糖+8g·L-1琼脂培养基有利于原球茎分化小苗, 低浓度6-BA和NAA组合适合原球茎增殖与芽的分化;在1/2MS+0.5mg·L-1 NAA+20g·L-1蔗糖+8g·L-1琼脂培养基上生根率达100%, 通过以上系列培养基的培养可获得完整植株。Abstract: To alleviate a seed shortage on Pholidota cantonensis Rolfe, artificial culturing with medium optimization was investigated by using the plant tissue culture technology.The results showed that MS+20g·L-1 sucrose+8g·L-1 agar was an appropriate medium that allowed a germination rate above 70%.MS+50g·L-1 potato+20g·L-1 sucrose+8g·L-1 agar was desirable for the plantlet′s protocorm differentiation.Low concentrations of 6-BA and NAA in medium was better for bud protocorm proliferation and differentiation.The medium formulated with 1/2MS+0.5mg·L-1 NAA+20g·L-1 sucrose+8g·L-1 agar was optimal for rooting, as a rate of 100%was achieved.By using the combination of the above-mentioned media at respective stages, the plantlets of P.cantonensis Rolfe could be rapidly obtained from seeds.
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Key words:
- Pholidota cantonensis Rolfe /
- seed /
- aseptic seeding /
- rapid propagation
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