摘要:
采用红曲霉S为供试菌株,研究几种维生素对其产色素能力及发酵液色调的影响,试验结果表明:烟酸和叶酸对红曲霉S产色素的促进作用最为明显,产品色价可达到80U·g-1左右。添加0.03%烟酸时可以得到高色价偏红色调的红曲色素产品,其色调值为1.40;在维生素单因素试验基础上采用二次通用旋转试验设计,对红曲霉的培养基配比进行优化,并建立二次回归方程。选择大米粉、味精、Mn2+、烟酸为试验因素,以色价为指标建立回归数学模型,通过试验结果进行方差分析及对数学模型进行优化得到红曲霉培养基的最佳配比。试验结果表明:当添加3.97%大米粉、3.37%味精、0.024%Mn2+、0.04%烟酸时,产品色价的理论值为107.3U·g-1,实际值为106.7U·g-1。
关键词:
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红曲霉 /
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维生素 /
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红曲色素 /
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色价
Abstract:
Monascus purpureus S was used to research the effects of different vitamins on pigment production and color hue of fermentation liquor.The result indicated that niacin and folic acid were obvious promoted pigment production,the color value was up to about 80U·g-1.The color tone value of Monascus pigment production was 1.40when adding 0.03% niacin,which would lead to obtain high color value with red tone.Based on vitamin single-factor experiment,the culture medium of Monascus was optimized by quadratic general rotary design and a quadratic regression equation was established.The optimum culture medium of Monascus was obtained by variance analysis and the optimization of mathematical model,which were 3.97% rice powder,3.37% monosodium glutamate,0.024% Mn2+and 0.04% vitamin PP.Under those conditions,the highest color value reached with estimated value and cerified value being of 107.3U·g-1 and 106.7U·g-1.
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